Analysis of fumonisins B1, B2 and their hydrolysed metabolites in pig liver by LC–MS/MS

Gazzotti, Teresa; Zironi, Elisa; Lugoboni, Barbara; Barbarossa, Andrea; Piva, Andrea; Pagliuca, Giampiero

doi:10.1016/j.foodchem.2010.10.009

Cited by 29 publications

(34 citation statements)

References 17 publications

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“…Recently, determination of fumonisins by LC/MS was significantly developed because of the high sensitivity and selectivity of the mass spectrometer (Gazzotti et al, 2011;Monbaliu et al, 2010;Ş enyuva & Gilbert, 2008). Fumonisins are detected by electrospray ionization (ESI) with such analyzers as singlequadrupole, triple-quadrupole, ion-trap and time-of-flight (Gazzotti et al, 2009;Li, Herrman, & Dai, 2010;Ş enyuva & Gilbert, 2008).…”

Section: Analytical Methods For Identification Of Fumonisinsmentioning

confidence: 99%

Occurrence of fumonisins in food – An interdisciplinary approach to the problem

2012

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Section: Analytical Methods For Identification Of Fumonisinsmentioning

confidence: 99%

Occurrence of fumonisins in food – An interdisciplinary approach to the problem

2012

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“…Also, in rat liver microsomes FB1 was scarcely metabolized (Merrill, Nikolova‐Karakashian, Schmelz, Morgan, & Stewart, ). As in the case of pigs (Gazzotti et al., ), there was a small conversion rate of FB1 to HFB1 (<1%), indicating that this process was not important during metabolism (Fodor et al., ) (Table ).…”

Section: Fumonisins (Fb)mentioning

confidence: 96%

“…FB incubation pinpointed a high stability in vitro in pigs and cows. In pig liver microsomes, only traces of HFB1 and HFB2 were observed (Gazzotti et al., ), while in bovine liver microsomes FB1 was not degraded or transformed to other compounds (Spotti, Pompa, & Caloni, ). Also, in rat liver microsomes FB1 was scarcely metabolized (Merrill, Nikolova‐Karakashian, Schmelz, Morgan, & Stewart, ).…”

Section: Fumonisins (Fb)mentioning

confidence: 99%

Mycotoxin Biomarkers of Exposure: A Comprehensive Review

Vidal

Mengelers

Yang³

et al. 2018

Comp Rev Food Sci Food Safe

160

137

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To date, the use of biomarkers has become generally accepted. Biomarker-driven research has been proposed as a successful method to assess the exposure to xenobiotics by using concentrations of the parent compounds and/or metabolites in biological matrices such as urine or blood. However, the identification and validation of biomarkers of exposure remain a challenge. Recent advances in high-resolution mass spectrometry along with new analytical (postacquisition data-mining) techniques will improve the quality and output of the biomarker identification process. Chronic or even acute exposure to mycotoxins remains a daily fact, and therefore it is crucial that the mycotoxins' metabolism is unravelled so more knowledge on biomarkers in humans and animals is acquired. This review aims to provide the scientific community with a comprehensive overview of reported in vitro and in vivo mycotoxin metabolism studies in relation to biomarkers of exposure for deoxynivalenol, nivalenol, fusarenon-X, T-2 toxin, diacetoxyscirpenol, ochratoxin A, citrinin, fumonisins, zearalenone, aflatoxins, and sterigmatocystin.

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“…Among the published methods for mycotoxin determination in biological samples, including HPLC [20,31,32], GC-MS/MS [33], LC-MS [34,35], LC-MS/MS [4,18,36,37], and LC-HRMS [38], LC-MS/MS provides remarkable selectivity, accuracy and sensitivity. Most of the methods were applied to detect common regulated mycotoxins [4,5,8,19,32,37,[39][40][41][42], employing various sample preparation strategies such as the "dilute and shoot" approach [4,5,37,41], QuEChERS [15], liquid-liquid extraction (LLE) [20], immunoaffinity (IAC) columns [41,42], solid phase extraction (SPE) [18], and various combinations of these techniques [13]. However, only a few methods targeted for Alternaria and emerging Fusarium mycotoxins, including ENNB [38,43], AOH [3], AOH and AME [15], TeA [44], ENNs, and BEA [7], for human biomonitoring.…”

Section: Introductionmentioning

confidence: 99%

Development of a Sensitive and Reliable UHPLC-MS/MS Method for the Determination of Multiple Urinary Biomarkers of Mycotoxin Exposure

Liu

Zhao

et al. 2020

Toxins

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A variety of mycotoxins from different sources frequently contaminate farm products, presenting a potential toxicological concern for animals and human. Mycotoxin exposure has been the focus of attention for governments around the world. To date, biomarkers are used to monitor mycotoxin exposure and promote new understanding of their role in chronic diseases. The goal of this research was to develop and validate a sensitive UHPLC-MS/MS method using isotopically-labeled internal standards suitable for accurate determination of 18 mycotoxin biomarkers, including fumonisins, ochratoxins, Alternaria and emerging Fusarium mycotoxins (fumonisin B1, B2, and B3, hydrolyzed fumonisin B1 and B2, ochratoxin A, B, and alpha, alternariol, alternariol monomethyl ether, altenuene, tentoxin, tenuazonic acid, beauvericin, enniatin A, A1, B, and B1) in human urine. After enzymatic digestion with β-glucuronidase, human urine samples were cleaned up using HLB solid phase extraction cartridges prior to instrument analysis. The multi-mycotoxin and analyte-specific method was validated in-house, providing satisfactory results. The method provided good linearity in the tested concentration range (from LOQ up to 25–500 ng/mL for different analytes), with R2 from 0.997 to 0.999. The limits of quantitation varied from 0.0002 to 0.5 ng/mL for all analytes in urine. The recoveries for spiked samples were between 74.0% and 133%, with intra-day precision of 0.5%–8.7% and inter-day precision of 2.4%–13.4%. This method was applied to 60 urine samples collected from healthy volunteers in Beijing, and 10 biomarkers were found. At least one biomarker was found in all but one of the samples. The high sensitivity and accuracy of this method make it practical for human biomonitoring and mycotoxin exposure assessment.

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Analysis of fumonisins B1, B2 and their hydrolysed metabolites in pig liver by LC–MS/MS

Cited by 29 publications

References 17 publications

Occurrence of fumonisins in food – An interdisciplinary approach to the problem

Occurrence of fumonisins in food – An interdisciplinary approach to the problem

Mycotoxin Biomarkers of Exposure: A Comprehensive Review

Development of a Sensitive and Reliable UHPLC-MS/MS Method for the Determination of Multiple Urinary Biomarkers of Mycotoxin Exposure

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