Analysis of functionalized polyethylene terephthalate with immobilized NTPDase and cysteine

Muthuvijayan, Vignesh; Gu, Jun; Lewis, Randy S.

doi:10.1016/j.actbio.2009.05.020

Cited by 28 publications

(36 citation statements)

References 57 publications

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“…Analysis of the FT‐IR spectra shown in Figure 1 for the surface modified PET mesh shows the ability of the carboxylation technique to introduce functional groups to the surface. The progression of the spectra is consistent with the theory presented by Muthuvijayan et al in that the hydroxylation step will add an O‐H group to the PET surface, followed by the transformation of that group to a COOH − during the carboxylation step 23. The FT‐IR spectra for the carboxylated PET also shows prominent peaks at around 1600 and 1400 cm −1 which could be attributed to NH bending, NO stretching, or NO bending and suggests presence of primary amine or nitro functional groups.…”

Section: Discussionsupporting

confidence: 90%

“…Initially, various methods of PET surface functionalization were considered for use in this study including hydrolysis, aminolysis, and plasma treatment, all of which have demonstrated the ability to functionalize polymer surfaces 24, 25. However, these treatments have also been shown to cause bulk material degradation, inducing stress cracking and pits, which can be observed with SEM 23, 26. The carboxylation technique was selected based on previous characterization studies showing carboxylated PET samples performing similarly to pristine PET samples in surface analysis studies and bulk tensile testing 23.…”

Section: Discussionmentioning

confidence: 99%

“…FT‐IR spectra were collected for each pristine PET sample for comparison to the modified PET. A carboxylation technique was modified from Muthuvijayan, et al and used to functionalize the PET surface 23. PET samples were first treated with a 50% v/v solution of acetone in deionized water for 24 h. Samples were then hydroxylated with an 18.5% formaldehyde solution in 1 M acetic acid for 4 h, followed by a carboxylation treatment of 1 M bromoacetic acid in 2 M NaOH for 18 h. All modification steps were carried out at room temperature on an orbital shaker table at 150 rpm.…”

Section: Methodsmentioning

confidence: 99%

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Development and in vitro studies of a polyethylene terephthalate‐gold nanoparticle scaffold for improved biocompatibility

et al. 2011

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Polyethylene terephthalate (PET) mesh is one of the most commonly used synthetic biomaterials for tension-free hernia repair. In an effort to improve the biocompatibility of PET mesh, gold nanoparticles (AuNP) in various concentrations were conjugated to the PET surface to develop PET-AuNP scaffolds. These novel scaffolds were characterized with Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), and differential scanning calorimetry (DSC) to assess the addition of functional groups, presence of AuNPs, and thermal stability of the modified PET mesh, respectively. The biocompatibility of the PET-AuNP scaffolds was evaluated through in vitro cell culture assays. The cellularity of cells exposed to the PET-AuNP scaffolds, as well as the scaffolds' ability to reduce reactive oxygen species, was assessed using L929 murine fibroblasts. Antimicrobial properties of AuNPs conjugated to PET mesh were tested against the bacteria Pseudomonas aeruginosa. Results from the FT-IR showed presence of COOH groups while SEM displayed bonding of AuNPs to the PET surface. DSC results indicated that the PET more than likely did not undergo any detrimental degradation due to the surface modification. Results from the in vitro studies showed that AuNPs, in optimal concentrations (1× concentrations), enhanced cellularity, reduced ROS, and reduced bacteria adhesion to PET. These studies demonstrated enhanced biocompatibility of the AuNP conjugated PET mesh over pristine PET mesh.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Development and in vitro studies of a polyethylene terephthalate‐gold nanoparticle scaffold for improved biocompatibility

et al. 2011

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“…In regard of PCL/keratin mats, the NO release was very low during the whole time. It maybe because the residual cysteine within keratin catalyzed the GSNO to release NO . The cysteine tends to be nitrosylated with GSNO to produce S ‐nitrosocystein (CySNO), which is unstable and release NO.…”

Section: Resultsmentioning

confidence: 99%

Electrospun PCL/keratin/AuNPs mats with the catalytic generation of nitric oxide for potential of vascular tissue engineering

Wan

Liu

Jin

et al. 2018

J Biomedical Materials Res

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Nitric oxide (NO)‐generating materials are beneficial for vascular tissue engineering (VTE) scaffold because the produced NO would enhance endothelial cells viability while inhibit smooth muscle cell (SMC) proliferation and reduce platelet adhesion, resulting in ideal hemocompatibility and endothelialization. Herein, poly(ε‐caprolactone) (PCL)/keratin biocomposite mats were first fabricated, followed by in situ gold (Au) nanoparticles loading to afford PCL/keratin/AuNPs mats. These mats were characterized using field emission scanning electron microscopy (FE‐SEM), X‐ray photoelectron spectroscopy (XPS), X‐ray diffraction (XRD), and thermogravimetric analysis (TGA). The PCL/keratin/AuNPs mats were demonstrated to be capable of catalyzing NO release in the mimicked blood microenvironments. The generated NO could enhance human umbilical vein endothelial cell growth and inhibit human umbilical arterial SMC viability. In addition, these mats maintained the antibacterial activity of Au nanoparticles with good blood compatibility. Taken together, these keratin‐based composite mats have potential usage in the VTE. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 3239–3247, 2018.

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“…13 These parameters were slightly modified to see their effect on polypropylene. The samples were immersed in different sodium hydroxide concentrations varying of 1, 2, and 4N for 5 h in an oven maintained at 50 C. The parameters were selected based on processes that have induced hydrolysis in certain other polymers.…”

Section: Sodium Hydroxide Treatmentmentioning

confidence: 99%

Altering surface characteristics of polypropylene mesh via sodium hydroxide treatment

Regis

Jassal

Mukherjee³

et al. 2012

J Biomedical Materials Res

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Incisional hernias represent a serious and common complication following laparotomy. The use of synthetic (e.g. polypropylene) meshes to aid repair of these hernias has considerably reduced recurrence rates. While polypropylene is biocompatible and has a long successful clinical history in treating hernias and preventing reherniation, this material may suffer some limitations, particularly in challenging patients at risk of wound failure due to, for example, an exaggerated inflammation reaction, delayed wound healing, and infection. Surface modification of the polypropylene mesh without sacrificing its mechanical properties, critical for hernia repair, represents one way to begin to address these clinical complications. Our hypothesis is treatment of a proprietary polypropylene mesh with sodium hydroxide (NaOH) will increase in vitro NIH/3T3 cell attachment, predictive of earlier and improved cell colonization and tissue integration of polypropylene materials. Our goal is to achieve this altered surface functionality via enhanced removal of chemicals/oils used during material synthesis without compromising the mechanical properties of the mesh. We found that NaOH treatment does not appear to compromise the mechanical strength of the material, despite roughly a 10% decrease in fiber diameter. The treatment increases in vitro NIH/3T3 cell attachment within the first 72 h and this effect is sustained up to 7 days in vitro. This research demonstrates that sodium hydroxide treatment is an efficient way to modify the surface of polypropylene hernia meshes without losing the mechanical integrity of the material. This simple procedure could also allow the attachment of a variety of biomolecules to the polypropylene mesh that may aid in reducing the complications associated with polypropylene meshes today.

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Analysis of functionalized polyethylene terephthalate with immobilized NTPDase and cysteine

Cited by 28 publications

References 57 publications

Development and in vitro studies of a polyethylene terephthalate‐gold nanoparticle scaffold for improved biocompatibility

Development and in vitro studies of a polyethylene terephthalate‐gold nanoparticle scaffold for improved biocompatibility

Electrospun PCL/keratin/AuNPs mats with the catalytic generation of nitric oxide for potential of vascular tissue engineering

Altering surface characteristics of polypropylene mesh via sodium hydroxide treatment

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