Analysis of Gamma Irradiated Fourth Generation Mutant of Rodent Tuber (Typhonium Flagelliforme Lodd.) Based on Morphology and Rapd Markers

Abstract

Rodent tuber (Typhonium flagelliforme Lodd.) is an herbal medicinal plant with anticancer activity. The genetic diversity of rodent tuber is low due to vegetative propagation. Somatic cell population of rodent tuber from Bogor had been irradiated with gamma ray to increase genetic diversity. There were 37 clones of first generation putative mutant (MV1) which had been analyzed based on morphological and RAPD markers. Out of those 37 MV1 clones, there were 17 clones which had undergone genetic mutation and had a diversified genetic profile. MV1 had been regenerated to fourth generation putative mutant clones (MV4). This research was aimed to analyze the mutation stability of MV4 based on morphological and RAPD markers. Clone 6-1-2 had the highest increase of the number of shoots and leaves than control and the other MV4 clones, with 4.7 and 19.7 shoots and leaves, respectively. Clone 6-1-1-6 obtained the highest increase in plant height than control and the other MV4 clones, i.e. 25.2 cm. Clone 6-9-5 had the weightiest fresh and dry weight, i.e. 41.67 gram and 12.01 gram respectively. RAPD molecular marker analysis of MV4 by using 15 primers had produced 64 polymorphic DNA bands out of 146 total bands. OPD-10 primer produced the highest number of polymorphic bands, i.e. 15 polymorphic bands out of 17 total bands with sizes 200-2000 bp. RAPD profile of MV4 had showed 5 main clusters at similiarity coefficient cut-off 0.91. Morphological characterization and RAPD analysis had proved the stability of genetic mutation of MV4.

Mutant Plant Tipobio Variety of Rodent Tuber (Typhonium Flagelliforme): Fatty Acids Compounds and in Vitro Anticancer Activity

“…The rodent tuber mutant plants have conducted research through in vitro biotechnology (somaclonal combination) with gamma ray irradiation to produce mutant plants. The results showed that the mutant plants have characteristics morphology changes in the number of leaves, plants height, and wide leaves [5]. The effect of gamma irradiation can also cause genetic changes or mutations.…”

Characterization and Investigation of Stigmasterol Isolated from Rodent Tuber Mutant Plant (<em>Typhonium flagelliforme</em>), Its Molecular Docking as Anticancer on MF-7 Cells

“…Gamma rays successfully irradiated T. flagelliforme plants through combined mutagenesis of somaclonal variations in vitro to increase the number of bioactive compounds contained therein. T. flagelliforme mutant plants have been detected to undergo genetic changes in the first generation (MV1) [5]. The second-generation (MV2) and third-generation (MV3) T. flagelliforme mutants have shown genetic changes through molecular RAPD by comparing wild-type [6].…”

“…T. flagelliforme mutant plants have been detected to undergo genetic changes in the first generation (MV1) [5]. The second-generation (MV2) and third-generation (MV3) T. flagelliforme mutants have shown genetic changes through molecular RAPD by comparing wild-type [6]. T. flagelliforme mutant plant received Plant Variety Protection by the Indonesian Ministry of Agriculture in 2020.…”

“…T. flagelliforme mutant plant received Plant Variety Protection by the Indonesian Ministry of Agriculture in 2020. T. flagelliforme mutant plant has been shown to have bioactive compounds, and its potential as an anticancer agent has increased [6][7][8]. The ethyl acetate fraction of the superior T. flagelliforme mutant has potential as an anticancer with an IC50 value of 1.09 µg/mL.…”

In Vitro Cytotoxic Activity of Rodent Tuber Mutant Plant (Typhonium Flagelliforme Lodd.) Against to McF-7 Breast Cancer Cell Line

“…In vitro mutagenesis was carried out using a combination of gamma-ray irradiation and somaclonal variation to increase the bioactive compounds in rodent tuber plants. The rodent tuber mutant plants have morphology changes [7], genetic changes [8,9], and protein expression changes [10]. Morphological, genetic, and protein expression changes can reinforce the bioactive compounds in mutant plants.…”