Analysis of gene expression in two human‐derived cell lines exposed <b><i>in vitro</i></b> to a 1.9 GHz pulse‐modulated radiofrequency field

Chauhan, Vinita; Qutob, Sami S.; Lui, Stephanie; Mariampillai, Anusiyanthan Isaac; Bellier, Pascale V.; Yauk, Carole L.; Douglas, George R.; Williams, Andrew; McNamee, James P.

doi:10.1002/pmic.200700215

Cited by 25 publications

(20 citation statements)

References 36 publications

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“…In addition, the data are very heterogeneous, making any comparison difficult. Several publications showed no significant difference between sham or exposed cells [Gurisik et al, 2006;Qutob et al, 2006;Whitehead et al, 2006a;Chauhan et al, 2007;Huang et al, 2008], or described weak changes that were not confirmed by RT-PCR validation [Zeng et al, 2006;Paparini et al, 2008;Roux et al, 2011;Sakurai et al, 2011], while other microarray studies reported modified gene expression after radiofrequency exposure [Belyaev et al, 2006;Nylund and Leszczynski, 2006;Remondini et al, 2006;Zhao et al, 2007;Huang et al, 2008]. However, these positive studies reported a small number of responsive genes (9-34) with a FC generally close to, or lower than 2.…”

Section: Discussionmentioning

confidence: 80%

Whole‐genome expression analysis in primary human keratinocyte cell cultures exposed to 60 GHz radiation

Quément

Nicolaz

Desmots

et al. 2011

Bioelectromagnetics

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The main purpose of this study is to investigate potential responses of skin cells to millimeter wave (MMW) radiation increasingly used in the wireless technologies. Primary human skin cells were exposed for 1, 6, or 24 h to 60.4 GHz with an average incident power density of 1.8 mW/cm(2) and an average specific absorption rate of 42.4 W/kg. A large-scale analysis was performed to determine whether these exposures could affect the gene expression. Gene expression microarrays containing over 41,000 unique human transcript probe sets were used, and data obtained for sham and exposed cells were compared. No significant difference in gene expression was observed when gene expression values were subjected to a stringent statistical analysis such as the Benjamini-Hochberg procedure. However, when a t-test was employed to analyze microarray data, 130 transcripts were found to be potentially modulated after exposure. To further quantitatively analyze these preselected transcripts, real-time PCR was performed on 24 genes with the best combination of high fold change and low P-value. Five of them, namely CRIP2, PLXND1, PTX3, SERPINF1, and TRPV2, were confirmed as differentially expressed after 6 h of exposure. To the best of our knowledge, this is the first large-scale study reporting on potential gene expression modification associated with MMW radiation used in wireless communication applications.

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Section: Discussionmentioning

confidence: 80%

Whole‐genome expression analysis in primary human keratinocyte cell cultures exposed to 60 GHz radiation

Quément

Nicolaz

Desmots

et al. 2011

Bioelectromagnetics

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“…The expression of hsp70 mRNA in radiofrequency exposed groups was due to the effect of non thermal, non ionizing radiation fields as no expression of such gene was observed in unexposed groups which was incubated at 37 O C. The expression of hsp70 gene was well the stress gene hsp70 upon in vitro culture of lymphocytes of both the breeds as compared to higher RF documented in human glioblastoma derived celllines (U87MG) in response to exposure of radiofrequency field (Chauhan et al, 2007). In the present study, lower radiofrequency exposure (850 MHz) caused early expression of exposure (1200 MHz) indicating lower RF exposure was more stressful to immune effector cells especially on peripheral lymphocytes.…”

Section: Discussionmentioning

confidence: 93%

In vitro Effect of Radiofrequency on hsp70 Gene Expression and Immune-effector Cells of Birds

Pk¹,

Jana²,

Thulasiraman³

et al. 2014

Adv. Anim. Vet. Sci.

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Poultry birds under free range system of management are a good biological indicator for lowintensity Electro-Magnetic Frequency radiations (EMF-r) for their vulnerability to EMF-r. The effect of EMF-r on different biological systems was extensively studied but no consistent effects had been inferred in this regard. With the aim of assessing the in vitro effect of radio frequency (RF) exposure on cellular stress and on functional activities of immune-effectors cells of poultry, this study was conducted. Two rural breeds [one best adapted exotic breed-Rhode Island Red (RIR) and one indigenous breed-Haringhata Black (HB)] were used. Blood samples, collected from six healthy birds of each species, were used for neutrophil functional assay by nitro blue terazolium (NBT) reduction test. Peripheral blood mononuclear cells (PBMC), obtained from blood were used for lymphoproliferation assay by 3 -[4, 5 -dimethlythiazol-2yl] -2, 5 -di phenyltetrazolium bromide (MTT dye) and expression of hsp70 gene by reverse transcription-polymerase chain reaction (RT-PCR) analysis. Prior to the experiment, PBMC were cultured into tissue culture plates and blood for NBT assay was taken in separate micro-centrifuge tubes from the sample of each bird in duplicates under bio-safety cabinet for radiofrequency exposure. The plates and tubes were exposed to 850 and 1200 MHz radiofrequency field from a standard mobile phone separately along with a power analyzer (SAR 1.7 W/Kg) for 5 minutes, 15 minutes, 25 minutes, 40 minutes and 60 minutes. The common trend of the hsp70 gene expression in RIR and HB breed of birds were observed upon 850 RF exposures from 25 minutes onward. The results showed that upon 1200 MHz RF post exposure, hsp70 gene expression occurred at 25 minutes onward in HB breed birds and 60 minutes post exposure in case of RIR bird. A typical trend of initial significant (P ≤ 0.05) decrease (5 minutes through 15 minutes post exposure) followed by significant (P ≤ 0.05) increase in lymphoproliferation from 25 minutes to 60 minutes, was observed in both the breeds and magnitude of exposures. All the test groups no significant variation in superoxide production by neutrophil. It may be concluded that, lower radiofrequency (850 MHz) emitted by mobile phone poses more stressful effect than the higher radiofrequency (1200 MHz) on in vitro exposed cells in both the breeds as indicated by expression of hsp70 gene and initial suppression of proliferation ability.All copyrights reserved to Nexus® academic publishers

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“…In contrast, Whitehead et al (2006aWhitehead et al ( , 2006b, Qutob et al (2006), Hirose et al (2006), Zeng et al (2006), and Chauhan et al (2007a) employed high-throughput DNA microarrays to assess global gene expression patterns in exposed cell lines and found no evidence that RF radiation at different frequencies could alter gene expression. Whitehead et al (2006b), Qutob et al (2006), and Chauhan et al (2007a) emphasized that there is a high chance of false positives using such highthroughput techniques because of the large number of genes tested.…”

Section: Gene Expressionmentioning

confidence: 88%

“…Whitehead et al (2006b), Qutob et al (2006), and Chauhan et al (2007a) emphasized that there is a high chance of false positives using such highthroughput techniques because of the large number of genes tested. It was pointed out that it is important to use repeated RF exposures with matched shams, a sham versus sham analysis, and a comparison to a matched control in order to properly account for biological variability where normal fluctuations in gene expression levels are known to occur.…”

Section: Gene Expressionmentioning

confidence: 97%

Recent Advances in Research on Radiofrequency Fields and Health: 2004–2007

Habash

Elwood

Krewski

et al. 2009

Journal of Toxicology and Environmental Health, Part B

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The widespread use of wireless telecommunications devices, particularly mobile phones and wireless networks, has resulted in increased human exposure to radiofrequency (RF) fields. Although national and international agencies have established safety guidelines for exposure to RF fields, concerns remain about the potential for adverse health outcomes to occur in relation to RF field exposure. The extensive literature on RF fields and health was reviewed by a number of authorities, including the Royal Society of Canada (1999). This report is the third in a series of updates to the original report of the Royal Society of Canada, covering the period 2004-2007. In particular, the present study examined new data on (1) dosimetry and exposure assessment, (2) biological effects of RF fields such as enzyme induction, and (3) toxicological effects, including genotoxicity and carcinogenicity. Epidemiological studies of the potential health effects of RF exposure, particularly from mobile phones, were determined, along with human and animal studies of neurological and behavioural effects. Within the last 4 yrs investigators concluded that there is no clear evidence of adverse health effects associated with RF fields, although continued research is recommended to address specific areas of concern, including exposure to RF fields among children using mobile phones. The results of the ongoing 13-country World Health Organization INTERPHONE study of mobile phones may provide important new information on the potential cancer risks associated with mobile phone use.

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Analysis of gene expression in two human‐derived cell lines exposed in vitro to a 1.9 GHz pulse‐modulated radiofrequency field

Cited by 25 publications

References 36 publications

Whole‐genome expression analysis in primary human keratinocyte cell cultures exposed to 60 GHz radiation

Whole‐genome expression analysis in primary human keratinocyte cell cultures exposed to 60 GHz radiation

In vitro Effect of Radiofrequency on hsp70 Gene Expression and Immune-effector Cells of Birds

Recent Advances in Research on Radiofrequency Fields and Health: 2004–2007

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