Analysis of genes isolated from lipopolysaccharide-stimulated rainbow trout (Oncorhynchus mykiss) macrophages

Goetz, Frederick W.; Iliev, Dimitar B.; McCauley, Linda; Liarte, C.; Tort, L.; Planas, Josep V.; Mackenzie, Simon

doi:10.1016/j.molimm.2004.06.005

Cited by 93 publications

(51 citation statements)

References 76 publications

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“…This could be due to more extensive inflammatory processes in the peritoneal cavity, where the spleen is situated and the bacterin was injected. terial DNA stimulate rainbow trout head-kidney macrophages to induce IL-1β expression and stimulate peripheral blood (PBL), spleen, and head-kidney lymphocytes to proliferate (Jørgensen et al 2001, Goetz et al 2004). This cytokine is a potent pro-inflammatory molecule in fish (Lindenstrøm et al 2004), and it increases leukocyte migration in rainbow trout (Hong et al 2003).…”

Section: Discussionmentioning

confidence: 99%

Temperature-dependent expression of immune-relevant genes in rainbow trout following Yersinia ruckeri vaccination

Raida¹,

Buchmann²

2007

Dis. Aquat. Org.

112

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The gene expression of immune-relevant genes in rainbow trout Oncorhynchus mykiss following vaccination with a bacterin of Yersinia ruckeri, a bacterial pathogen causing enteric red mouth disease (ERM), was investigated at 5, 15, and 25°C. Rainbow trout were immunized by i.p. injection of a water-based Y. ruckeri (serotype O1) bacterin, and gene expression profiles were compared to control groups injected with phosphate buffered saline (PBS). Blood and tissue samples (spleen and head kidney) were taken for subsequent analysis using solid phase enzyme-linked immunosorbent assay (ELISA) and real-time PCR, respectively. The up-regulation of cytokine genes was generally faster and higher at high water temperature, with major expression at 25°C. The proinflammatory cytokine interleukin (IL)-1β and interferon (IFN)-γ were significantly up-regulated in all immunized groups, whereas the cytokine IL-10 was only up-regulated in fish kept at 15 and 25°C. The gene encoding the C5a (anaphylatoxin) receptor was expressed at a significantly increased level in both head kidney and spleen of immunized fish. The secreted immunoglobulin M (IgM)-encoding gene was significantly up-regulated in the head kidney of immunized trout reared at 25°C, and a positive correlation (r = 0.663) was found between gene expression of secreted IgM in the head kidney and Y. ruckeri-specific antibodies in plasma measured by ELISA. However, no regulation of the teleost specific immunoglobulin T (IgT), which was generally expressed at a much lower level than IgM, could be detected. The study indicated that expression of both innate and specific adaptive immune-response genes are highly temperature-dependent in rainbow trout.

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Section: Discussionmentioning

confidence: 99%

Temperature-dependent expression of immune-relevant genes in rainbow trout following Yersinia ruckeri vaccination

Raida¹,

Buchmann²

2007

Dis. Aquat. Org.

112

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“…The percentages of the significant ESTs obtained were higher than for cDNA or subtracted libraries after experimental infection or immune stimulation of Japanese flounder (Paralichthys olivaceus) (30), carp (Cyprinus carpio) (31), salmon (S. salar) (32), and gilthead seabream (Sparus aurata) (33). Within the genes related to the immune system category, however, we identified genes that had already been described in those fish libraries (Table I) and are critical components in the mammalian immune response such as galectins (34,35), Cd209e (35), C-type lectins (31,32,34,36,37), pentraxins (32,38), MHC class II and heat-shock protein 90 (30,32,35), ␤ 2 -microglobulin, Ig H and L chains (30,32), and Tcomplex protein (32). This considerable number of common upregulated transcripts supports the notion that multiple components of both innate and adaptative immune responses of fish have been conserved in the vertebrate lineages leading to the mammals.…”

Section: Discussionmentioning

confidence: 91%

Nodavirus Infection of Sea Bass (Dicentrarchus labrax) Induces Up-Regulation of Galectin-1 Expression with Potential Anti-Inflammatory Activity

Poisa-Beiro

Dios

Ahmed

et al. 2009

The Journal of Immunology

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Sea bass nervous necrosis virus is the causative agent of viral nervous necrosis, a disease responsible of high economic losses in larval and juvenile stages of cultured sea bass (Dicentrarchus labrax). To identify genes potentially involved in antiviral immune defense, gene expression profiles in response to nodavirus infection were investigated in sea bass head kidney using the suppression subtractive hybridization (SSH) technique. A total of 8.7% of the expressed sequence tags found in the SSH library showed significant similarities with immune genes, of which a prototype galectin (Sbgalectin-1), two C-type lectins (SbCLA and SbCLB) from groups II and VII, respectively, and a short pentraxin (Sbpentraxin) were selected for further characterization. Results of SSH were validated by in vivo up-regulation of expression of Sbgalectin-1, SbCLA, and SbCLB in response to nodavirus infection. To examine the potential role(s) of Sbgalectin-1 in response to nodavirus infection in further detail, the recombinant protein (rSbgalectin-1) was produced, and selected functional assays were conducted. A dose-dependent decrease of respiratory burst was observed in sea bass head kidney leukocytes after incubation with increasing concentrations of rSbgalectin-1. A decrease in IL-1β, TNF-α, and Mx expression was observed in the brain of sea bass simultaneously injected with nodavirus and rSbgalectin-1 compared with those infected with nodavirus alone. Moreover, the protein was detected in the brain from infected fish, which is the main target of the virus. These results suggest a potential anti-inflammatory, protective role of Sbgalectin-1 during viral infection.

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“…Although acute phase proteins are synthesized predominantly in the liver (Talbot et al, 2009), our results show SAA expression profile to be similar in the spleen of fed charr in response to LPS stimulation. This is not surprising since recent studies have provided evidence of extrahepatic synthesis and cellular expression of APPs (Colten, 1992;Goetz et al, 2004;SaranyaRevathy et al, 2012). However, the faster SAA response to LPS stimulation in the spleen of fasted charr along with the cytokine response leads us to propose a paracrine and/or autocrine role for cytokines in APP response in this tissue.…”

Section: Innate Immune Modulationmentioning

confidence: 91%

Tissue-specific molecular immune response to lipopolysaccharide challenge in emaciated anadromous Arctic charr

Philip

Jørgensen

Maule³

et al. 2014

Developmental & Comparative Immunology

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Analysis of genes isolated from lipopolysaccharide-stimulated rainbow trout (Oncorhynchus mykiss) macrophages

Cited by 93 publications

References 76 publications

Temperature-dependent expression of immune-relevant genes in rainbow trout following Yersinia ruckeri vaccination

Temperature-dependent expression of immune-relevant genes in rainbow trout following Yersinia ruckeri vaccination

Nodavirus Infection of Sea Bass (Dicentrarchus labrax) Induces Up-Regulation of Galectin-1 Expression with Potential Anti-Inflammatory Activity

Tissue-specific molecular immune response to lipopolysaccharide challenge in emaciated anadromous Arctic charr

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