Analysis of Genetic Diversity amongIxoraCultivars (Rubiaceae) using Random Amplified Polymorphic DNA

Abstract

We have optimized the genomic DNA extraction method from fresh and dry laminas, as well as fresh and dry corolla lobes of Ixora cultivars. Some woody tropical species such as these contain excessive amounts of phenolic compounds that co-precipitate with DNA resulting in poor or no amplification during the polymerase chain reaction (PCR). Repeated precipitation with CsCl coupled with phenol : chloroform extraction yielded high quality DNA suitable for consistent PCR amplification. The DNA from fresh laminas of 22 cultivars of Ixora was subjected to random amplified polymorphic DNA (RAPD) analysis. Individual taxa could be identified using specific DNA markers from the RAPD profiles. Cluster analysis of data from six primers grouped all 22 cultivars distinctly under two cultivar groups, viz., Ixora Coccinea and I. Javanica. The percentage genetic similarity was calculated for all the cultivars based on the RAPD data. The two cultivar groups and the outgroup plants were also clearly distinguishable with polar ordination using a matrix of genetic dissimilarities (one minus similarity). Our data indicate that besides the use of RAPD markers for identification of particular Ixora cultivars within a germplasm collection, the phylogenetic relationships generated by RAPD analysis may be useful for future breeding programmes.# 1997 Annals of Botany Company

Assessment of genetic diversity in a highly valuable medicinal plant Catharanthus roseus using molecular markers

“…Thus, on the basis of RAPD, the findings of this study are similar to observations of Rajaseger et al (1997 and. Our Table 2.…”

Identification and genetic variation among eight varieties of ginger by using random amplified polymorphic DNA markers

“…'Nahfrey' had 64% similarity with 'Jugijan', 'Turia local', 'Nadia' and 'ZO-17'. The differences in number of individuals estimated by RAPD markers in this study are similar to the result obtained by Rajaseger et al (1997) in RAPD studies of the Ixora coccinea and I. javanica. They also found that the taxa-specific RAPD bands could be utilized to define the identification.…”

Phylogenic Study of Twelve Species of Phyllanthus Originated from India through Molecular Markers for Conservation

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