Analysis of Genetic Diversity in Napier Grass (Pennisetum purpureum Schum) as Detected by RAPD and ISSR Markers

Babu, C.; Sundaramoorthi, J.; Vijayakumar, G.; Ram, S. Ganesh

doi:10.1007/bf03263317

Cited by 20 publications

(16 citation statements)

References 20 publications

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“…Smith and co-workers [40] were the first group to develop and use molecular restriction fragment length polymorphism (RFLP) and random amplification of polymorphic DNA (RAPD), markers in Napier grass studies and they were able to link quantitative trait loci to several plant traits. A diversity analysis, based on RAPD molecular markers, revealed a moderate level of diversity with clear differentiation of Napier grass accessions from pearl millet and its hybrids, and the accessions were clustered into groups according to their geographical origin [4,41]. However, the difficulty of differentiating some of the accessions based on their RAPD profile was also acknowledged [4].…”

Section: Genetic Resources Molecular Diversity and Breedingmentioning

confidence: 99%

“…Interestingly, these results also did not reveal the clustering of different accessions according to their geographical origin, which was demonstrated by the previous morphological, agronomic and RAPD marker studies. Other types of molecular markers, such as inter-sequential simple repeat (ISSR) markers [41,45] and sequence-related amplified polymorphisms (SRAPs) [46] have also been used for the characterization and identification of Napier grass clones.…”

Section: Genetic Resources Molecular Diversity and Breedingmentioning

confidence: 99%

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Opportunities for Napier Grass (Pennisetum purpureum) Improvement Using Molecular Genetics

Negawo¹,

Teshome²,

Kumar

et al. 2017

Agronomy

102

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Napier grass (Pennisetum purpureum Schumach.) is a fast-growing perennial grass native to Sub-Saharan Africa that is widely grown across the tropical and subtropical regions of the world. It is a multipurpose forage crop, primarily used to feed cattle in cut and carry feeding systems. Characterization and diversity studies on a small collection of Napier grasses have identified a moderate level of genetic variation and highlighted the availability of some good agronomic traits, particularly high biomass production, as a forage crop. However, very little information exists on precise phenotyping, genotyping and the application of molecular technologies to Napier grass improvement using modern genomic tools which have been applied in advancing the selection and breeding of important food crops. In this review paper, existing information on genetic resources, molecular diversity, yield and nutritional quality of Napier grass will be discussed. Recent findings on characterizing disease resistance and abiotic stress (drought) tolerance will also be highlighted. Finally, opportunities and future prospects for better conservation and use arising from the application of modern genomic tools in Napier grass phenotyping and genotyping will be discussed.

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Section: Genetic Resources Molecular Diversity and Breedingmentioning

confidence: 99%

Section: Genetic Resources Molecular Diversity and Breedingmentioning

confidence: 99%

Opportunities for Napier Grass (Pennisetum purpureum) Improvement Using Molecular Genetics

Negawo¹,

Teshome²,

Kumar

et al. 2017

Agronomy

102

View full text Add to dashboard Cite

show abstract

“…However, these hybrids are sterile and need to be propagated vegetative, which puts a major limitation on their easy distribution to farmers. Babu et al (2009) [2], Randomly Amplified Polymorphic DNA (RAPD) and Inter-Simple Sequence Repeat (ISSR) markers were used to detect the DNA polymorphism among thirty Napier grass collections of wide geographical distribution maintained at TNAU, Coimbatore.…”

Section: Related Workmentioning

confidence: 99%

“…Naive Bayes algorithm is used for specifying all the correctly and incorrectly classified instances along with number of records and accuracy percentage for the same. The correctly classified Naive Bayes Classifiers' percentages of the attributes are listed in the table (2). …”

Section: Based On Naive Bayesian Classification Algorithmmentioning

confidence: 99%

Evaluating the Yield of Hybrid Napier Grass with Data Mining Techniques

Nadiammai¹,

Krishnaveni²,

Hemalatha³

2011

IJCA

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Data Mining is the process of identifying the hidden patterns from large amount of data. It is commonly used in a marketing, surveillance, fraud detection and scientific discovery. In data mining, machine learning techniques are mainly focused as research through which we learnt to recognize complex and make intelligent decisions based on data. This paper involves the information about the yield of the hybrid grass from NBH1 to NBH11.

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“…RFLP (restriction fragment length polymorphism; Smith et al, 1993), RAPD (random amplified polymorphic DNA; Smith et al, 1993;Daher et al, 2002;Lowe et al, 2003;Passos et al, 2005;Pereira et al, 2008;Babu et al, 2009), AFLP (amplified fragment length polymorphism; Harris et al, 2009), and ISSR (inter-simple sequence repeats; Babu et al, 2009) are examples of such use.…”

Section: Introductionmentioning

confidence: 99%

RAPD and ISSR markers in the evaluation of genetic divergence among accessions of elephant grass

Lima

Daher²,

Gonçalves³

et al. 2011

Genet. Mol. Res.

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ABSTRACT. Considering the expected genetic variability of elephant grass (Pennisetum purpureum), due to its cultivation in different continents, we characterized and estimated the genetic divergences between 46 accessions of elephant grass with different edaphoclimatic adaptations, using RAPD and ISSR markers. We evaluated, comparatively, the consistency of the information achieved with these markers. Twenty-six RAPD and 25 ISSR primers were employed. The RAPD markers produced 185 bands, 72% of which were polymorphic, with a mean of 5.11 polymorphic bands per primer. The 25 ISSR starters produced 216 bands; 76% were polymorphic, with a mean of 6.56 polymorphic bands per primer. The correlation between the genetic distances achieved by the RAPD and ISSR markers was 0.76, which is highly significant by the Mantel test. Based on UPGMA grouping, considering the point of sudden change, five and six groups were formed for the data from the RAPD and ISSR markers, respectively. Genetic diversity in elephant grass These markers provided partially concordant groups, indicating that these techniques can provide consistent information and consequently could be used in studies of genetic diversity among accessions.

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Analysis of Genetic Diversity in Napier Grass (Pennisetum purpureum Schum) as Detected by RAPD and ISSR Markers

Cited by 20 publications

References 20 publications

Opportunities for Napier Grass (Pennisetum purpureum) Improvement Using Molecular Genetics

Opportunities for Napier Grass (Pennisetum purpureum) Improvement Using Molecular Genetics

Evaluating the Yield of Hybrid Napier Grass with Data Mining Techniques

RAPD and ISSR markers in the evaluation of genetic divergence among accessions of elephant grass

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