RAPD and ISSR markers in the evaluation of genetic divergence among accessions of elephant grass

Abstract

ABSTRACT. Considering the expected genetic variability of elephant grass (Pennisetum purpureum), due to its cultivation in different continents, we characterized and estimated the genetic divergences between 46 accessions of elephant grass with different edaphoclimatic adaptations, using RAPD and ISSR markers. We evaluated, comparatively, the consistency of the information achieved with these markers. Twenty-six RAPD and 25 ISSR primers were employed. The RAPD markers produced 185 bands, 72% of which were polymorphic, with a mean of 5.11 polymorphic bands per primer. The 25 ISSR starters produced 216 bands; 76% were polymorphic, with a mean of 6.56 polymorphic bands per primer. The correlation between the genetic distances achieved by the RAPD and ISSR markers was 0.76, which is highly significant by the Mantel test. Based on UPGMA grouping, considering the point of sudden change, five and six groups were formed for the data from the RAPD and ISSR markers, respectively. Genetic diversity in elephant grass These markers provided partially concordant groups, indicating that these techniques can provide consistent information and consequently could be used in studies of genetic diversity among accessions.

Opportunities for Napier Grass (Pennisetum purpureum) Improvement Using Molecular Genetics

“…Interestingly, these results also did not reveal the clustering of different accessions according to their geographical origin, which was demonstrated by the previous morphological, agronomic and RAPD marker studies. Other types of molecular markers, such as inter-sequential simple repeat (ISSR) markers [41,45] and sequence-related amplified polymorphisms (SRAPs) [46] have also been used for the characterization and identification of Napier grass clones.…”

Estimates of heterosisparameters in elephant grass (Pennisetumpurpureum Schumach.) for bioenergy production

“…The female parents were selected based on late flowering, high DM yield, stem diameter (Rossi, 2010), and genetic divergence in relation to male parents, in accordance with Lima et al (2011). Male parents were selected based on the genetic divergence in relation to the female parents, and genetic divergence in relation to female parents (Lima et al, 2011), and also based on their morpho-agronomic traits (DM yield, stem diameter, number of tillers) (Rossi, 2010).…”

“…Male parents were selected based on the genetic divergence in relation to the female parents, and genetic divergence in relation to female parents (Lima et al, 2011), and also based on their morpho-agronomic traits (DM yield, stem diameter, number of tillers) (Rossi, 2010). Among these genotypes, ten parents were chosen, five females (P1, P2, P3, P4, and P5) (group I) and five males (P6, P7, P8, P9, and P10) (group II), forming a 5 × 5 partial diallel (Table 1).…”

“…The greater the divergence between the parents used in most crossing is expected heterosis. This divergence can be estimated with the use of molecular markers (Lima et al, 2011). Therefore, these analyses are important in choosing the method to direct populations in a breeding program (Pimentel et al, 2013).…”

Inter- and intra-specific genetic diversity of Iranian yarrow species Achillea santolina and Achillea tenuifolia based on ISSR and RAPD markers

“…It has been shown that different markers might reveal different kinds of variation (Powell et al, 1996). RAPD markers (Williams et al, 1990) and ISSR markers (Zietkiewicz et al, 1994) have been used with success to identify and determine relationships at the species, population, and cultivar levels in many plant species, including several aromatic and medicinal plants (Mattioni et al, 2002;Nan et al, 2003;Fracaro et al, 2005;Manica-Cattani et al, 2009;de Lima et al, 2011). However, there are limited reports on the genetic relationships of Achillea species.…”