Analysis of genetic markers of N. Gonorrhoeae resistance to β-lactam antibiotics

Malakhova, Maja V.; Vereshchagin, V. A.; Ilina, Elena; Govorun, Vadim M.; Зубков, М. М.; Priputnevich, Tatiana V.; Kisina, V. I.; Кубанова, А. А.

doi:10.1007/s10517-006-0234-9

Cited by 6 publications

(4 citation statements)

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“…Penicillin resistance of N. gonorrhoeae was evaluated by genetic analysis of bla, por, ponA, and penA genes [3]. Gonococcus genotype was determined by evaluating the por gene nucleotide sequence [2].…”

Section: Methodsmentioning

confidence: 99%

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Direct evaluation of drug resistance parameters in gonococcus

et al. 2007

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We carried out complex genetic analysis of clinical samples containing N. gonorrhoeae DNA, the genotype and profile of drug resistance of this agent were evaluated. Changes in genes responsible for the formation of N. gonorrhoeae resistance to penicillins, fluoroquinolones, and spectinomycin were detected during minisequencing with subsequent MALDI-TOF mass spectrometry. The sensitivity of gonococcus was evaluated directly in the clinical sample without culturing.

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Section: Methodsmentioning

confidence: 99%

“…A correlation between PC and fluoroquinolone (FQ) resistance of N. gonorrhoeae strains and genetic changes in the corresponding locuses of the bacterial genome was previously demonstrated [1,3].…”

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confidence: 94%

Direct evaluation of drug resistance parameters in gonococcus

et al. 2007

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“…Mutations in the penA and ponA genes were detected in order to analyze ␤-lactam resistance in Neisseria gonorrhoeae (69), and subsequent works described the detection of resistance to fluoroquinolones, spectinomycin, and tetracycline (70,71). That same group reported MALDI-TOF MS minisequencing for the detection of resistance in Streptococcus pneumoniae (72,73).…”

Section: Minisequencingmentioning

confidence: 99%

Matrix-Assisted Laser Desorption Ionization–Time of Flight (MALDI-TOF) Mass Spectrometry for Detection of Antibiotic Resistance Mechanisms: from Research to Routine Diagnosis

2013

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SUMMARYMatrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) has been successfully applied as an identification procedure in clinical microbiology and has been widely used in routine laboratory practice because of its economical and diagnostic benefits. The range of applications of MALDI-TOF MS has been growing constantly, from rapid species identification to labor-intensive proteomic studies of bacterial physiology. The purpose of this review is to summarize the contribution of the studies already performed with MALDI-TOF MS concerning antibiotic resistance and to analyze future perspectives in this field. We believe that current research should continue in four main directions, including the detection of antibiotic modifications by degrading enzymes, the detection of resistance mechanism determinants through proteomic studies of multiresistant bacteria, and the analysis of modifications of target sites, such as ribosomal methylation. The quantification of antibiotics is suggested as a new approach to study influx and efflux in bacterial cells. The results of the presented studies demonstrate that MALDI-TOF MS is a relevant tool for the detection of antibiotic resistance and opens new avenues for both clinical and experimental microbiology.

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“…For detection of mutations in MtrRCDE locus and rpsJ gene we used an approach based on minisequence reaction followed by identification of products by MALDI-TOF MS (matrix-assisted laser desorption time-of-flight mass spectrometry). The complex analysis of genetic markers was successfully used for the evaluation of N. gonorrhoeae resistance to fluoroquinolones and β-lactam antibiotics [1,3].…”

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confidence: 99%

Analysis of the contribution of molecular mechanisms into formation of gonoccocal resistance to tetracycline

Borovskaya¹,

Malakhova²,

Vereshchagin³

et al. 2007

Bull Exp Biol Med

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We applied complex genetic analysis for evaluation of tetracycline-resistance markers in 129 clinical strains of Neisseria gonorrhoeae from Central, Privolzhskii, and Siberian regions. For detection of mutations in rpsJ gene and MtrRCDE locus we first used minisequence reaction followed by identification of products by MALDI-TOF mass spectrometry. The incidence of detection of resistance markers among the analyzed strains were: tetM--3.1%, mutations in genes rpsJ--82.2%, penB--62.8%, and mtrR--54.3%. The analyzed genetic markers were not detected in 17.5% strains. tetM gene was detected in only 12.5% strains from the Central Region. No differences were revealed in regional distribution of other genotypes. Genotypes tetM(pres), rpsJ(mut), mtrR(mut), and rpsJ(mut), penB(mut), mtrR(mut) reliably predict tetracycline resistance. Microbiological and genetic testing of tetracycline resistance yielded similar results.

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Analysis of genetic markers of N. Gonorrhoeae resistance to β-lactam antibiotics

Cited by 6 publications

References 10 publications

Direct evaluation of drug resistance parameters in gonococcus

Direct evaluation of drug resistance parameters in gonococcus

Matrix-Assisted Laser Desorption Ionization–Time of Flight (MALDI-TOF) Mass Spectrometry for Detection of Antibiotic Resistance Mechanisms: from Research to Routine Diagnosis

Analysis of the contribution of molecular mechanisms into formation of gonoccocal resistance to tetracycline

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