Direct evaluation of drug resistance parameters in gonococcus

Ilina, Elena; Malakhova, Maja V.; Vereshchagin, V. A.; Govorun, Vadim M.; Priputnevich, Tatiana V.; Кубанова, А. А.

doi:10.1007/s10517-007-0296-3

Cited by 2 publications

(1 citation statement)

References 13 publications

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“…Mutations in the penA and ponA genes were detected in order to analyze ␤-lactam resistance in Neisseria gonorrhoeae (69), and subsequent works described the detection of resistance to fluoroquinolones, spectinomycin, and tetracycline (70,71). That same group reported MALDI-TOF MS minisequencing for the detection of resistance in Streptococcus pneumoniae (72,73).…”

Section: Minisequencingmentioning

confidence: 99%

Matrix-Assisted Laser Desorption Ionization–Time of Flight (MALDI-TOF) Mass Spectrometry for Detection of Antibiotic Resistance Mechanisms: from Research to Routine Diagnosis

2013

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SUMMARYMatrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) has been successfully applied as an identification procedure in clinical microbiology and has been widely used in routine laboratory practice because of its economical and diagnostic benefits. The range of applications of MALDI-TOF MS has been growing constantly, from rapid species identification to labor-intensive proteomic studies of bacterial physiology. The purpose of this review is to summarize the contribution of the studies already performed with MALDI-TOF MS concerning antibiotic resistance and to analyze future perspectives in this field. We believe that current research should continue in four main directions, including the detection of antibiotic modifications by degrading enzymes, the detection of resistance mechanism determinants through proteomic studies of multiresistant bacteria, and the analysis of modifications of target sites, such as ribosomal methylation. The quantification of antibiotics is suggested as a new approach to study influx and efflux in bacterial cells. The results of the presented studies demonstrate that MALDI-TOF MS is a relevant tool for the detection of antibiotic resistance and opens new avenues for both clinical and experimental microbiology.

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