Analysis of genetic variability in the Czech Dachshund population using microsatellite markers

Abstract

The primary goal of this study was to analyse genetic variation within and among six Dachshund varieties in the Czech Republic using 10 microsatellites from StockMarks Paternity Polymerase Chain Reaction (PCR) Typing kit. A total of 632 Dachshunds were sampled - 99 Standard Smooth-haired, 72 Standard Long-haired, 272 Standard Wire-haired, 42 Miniature Smooth-haired, 73 Miniature Long-haired and 74 Miniature Wire-haired. Average observed heterozygosity and polymorphic information content ranged in particular Dachshund varieties between 0.58-0.70 and 0.57-0.64, respectively. Subsequent analysis of the population structure (F-statistics; Nei's genetic identity) showed that Standard Dachshunds shared allele frequencies most closely with their miniature equivalents, and smooth coat type is closer to Wire-haired coat type than to the Long-haired one. Analysis of molecular variance revealed that 11.81% of the total variance occurred among varieties. The value of combined exclusion probability (0.9955) validated usefulness of this panel of microsatellites for parentage verification in all analysed populations. We carried out 234 parentage verifications with 1.28% cases where parentage did not match.

Genetic variability of the Bracco Italiano dog breed based on microsatellite polimorphism

“…The mean number of alleles per locus was 6.43 (SD=1.47). Although a comparison with other breeds can be biased due to the different marker sets used by different authors, it may be noted that this value is near the upper range of the published values for other breeds: Greyhound, 2.5 alleles/locus; Labrador Retriever 3.3; German Shepherd, 3.3 (Zajc et al, 1997);Flat-coated Retriever, 4.5;Dachshund, 5.6 (Fredholm and Wintero, 1995); Andalusian Hound, 6.25; Spanish Greyhound 6.5; Maneto, 7.0 (Morera et al, 1997); Czech Dachshunds, 7.6 alleles/locus (Pribánová et al, 2009); and 12 East Asian dog breeds, 7.75 (Kim et al, 2001).…”

Microsatellite loci analysis for individual identification in Shiba Inu

“…But, the average expected heterozygosity (He), polymorphic information content (PIC) and fixation index (F) calculated from the 16 microsatellite markers we analyzed were 0.665, 0.623 and 0.046, respectively. As the values were similar to research using different markers on Shiba Inus in Japan [ 8 ] as well as other popular breeds in other countries [ 3 , 10 , 15 , 17 ], it is suggested that the level of inbreeding in Shiba Inus is not so high as concerned.…”

Studies of modern Italian dog populations reveal multiple patterns for domestic breed evolution

“…We additionally examined a set of three breeds for which we collected samples in both the United States and Italy: Italian Greyhounds (Figure 1a), Cane Corsos (Figure 1v), and Neapolitan Mastiffs ( Figure 1w). As population substructure has previously been observed in international populations of breeds (Pedersen, Liu, Leonard, & Griffioen, 2015;Pedersen, Liu, McLaughlin, & Sacks, 2012;Quignon et al, 2007), we hope to characterize genetic divergence between US and Italian populations of Italian breeds, caused by importation bottlenecks, regional popular sire effects (Leroy & Baumung, 2010;Pribanova et al, 2009), or variation in selection. All three were correctly grouped by breed.…”

“…Smaller studies exist which have addressed population demography in globally recognized breeds sampled from discrete locations with some success (Bigi, Marelli, Randi, & Polli, 2015;Ceh & Dovc, 2014;Koskinen & Bredbacka, 2000;Oliehoek, Bijma, & van der Meijden, 2009;Parra, Mendez, Canon, & Dunner, 2008;Pertoldi et al, 2013;Pribanova et al, 2009;Suarez, Betancor, Fregel, & Pestano, 2013;Wiener et al, 2017). While such studies provide insight into the status and genetic health of local populations, they do not necessarily consider the preexisting breedspecific genomic patterns developed prior to localization or the impact of import and export from the local breeding pool.…”