Analysis of Genetic Variability in Long-term Callus Cultures and Regenerated Plants of Maize.

Molina, Marı́a del Carmen; García, María Dina

doi:10.1508/cytologia.63.183

Cited by 9 publications

(5 citation statements)

References 27 publications

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“…Again, an average coverage of 50-fold indicates hemizygous regions, while multiples of this hemizygous coverage value indicate the presence of additional copies. The high number of genes with increased copy numbers is in agreement with previous reports of increases in chromosome duplications over cultivation time as callus [52]. Analysis of the genome of this cell line again after additional years of cultivation could reveal if there is a saturation of this increase in ploidy and nuclear DNA content.…”

Section: Resultssupporting

confidence: 91%

Twenty-Five Years of Propagation in Suspension Cell Culture Results in Substantial Alterations of the Arabidopsis Thaliana Genome

Pucker

Rückert

Stracke

et al. 2019

Genes

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Arabidopsis thaliana is one of the best studied plant model organisms. Besides cultivation in greenhouses, cells of this plant can also be propagated in suspension cell culture. At7 is one such cell line that was established about 25 years ago. Here, we report the sequencing and the analysis of the At7 genome. Large scale duplications and deletions compared to the Columbia-0 (Col-0) reference sequence were detected. The number of deletions exceeds the number of insertions, thus indicating that a haploid genome size reduction is ongoing. Patterns of small sequence variants differ from the ones observed between A. thaliana accessions, e.g., the number of single nucleotide variants matches the number of insertions/deletions. RNA-Seq analysis reveals that disrupted alleles are less frequent in the transcriptome than the native ones.

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Section: Resultssupporting

confidence: 91%

Twenty-Five Years of Propagation in Suspension Cell Culture Results in Substantial Alterations of the Arabidopsis Thaliana Genome

Pucker

Rückert

Stracke

et al. 2019

Genes

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“…On average, among the RO regenerants 12% were aneuploid, 14% tetraploid and 4% were showing multivalent associations. Similar chromosomal instability in the regenerants has been reported in a number of Graminaceous species like oats (McCoy et al 1982), Triticale (Armstrong et al 1983), Lolium (Ahloowalia 1983), maize (Molina and Garcia 1998, Rhodes et al 1986, Lee and Phillips 1987 and wheat. The occurrence of plants with multivalent associations in the present study might indicate segmental homologies.…”

Section: Discussionsupporting

confidence: 75%

Regeneration and Analysis of Genetic Variability in Wild Sorghum, S. australiense Garber and Snyder.

2001

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“…Several authors have observed that chromosomic stability in vitro is affected by factors such as the explant, the genotype and, mainly, the culture age (McCoy and Phillips, 1982;Molina and García, 1998). Therefore, long-term organic calli could be useful to induce chromosomic alterations that increase the exchange frequency between the chromosomes of the parental species.…”

Section: Introductionmentioning

confidence: 99%

NaCl tolerance in maize (Zea mays ssp. mays) x Tripsacum dactyloides L. hybrid calli in regenerated plants

Pesqueira

García

Molina

2003

Span. j. agric. res.

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Since corn has been classified as one of the most sensitive crops to soil salinity, the evaluation of NaCl toleranceof a hybrid of Zea mays ssp. mays (2n=40) and Tripsacum dactyloides (2n=72), was considered of interest to determinethe possibility of using this germplasm in corn tolerance improvement. Long-term organogenic calli, obtainedfrom immature hybrid embryos, have shown high rates of multiplication and plant regeneration. After in vitro treatmentof these calli with different levels of salinity, the survival and regeneration percentages and the daily relativeweight increments were calculated. Regenerated plants were transplanted to pots and watered with NaCl solution (6.1dS m-1) in order to assess tolerance at a whole plant level. Measurements of height, number of leaves per plant, andfresh and dry weights were taken. Exposure to 170 mM NaCl in vitro during 35 days and in vivo during 20 days produceda fresh weight decrease of 51% in calli and 31% in plants, respectively. These results indicate an improved toleranceof the maize/Tripsacum hybrid to salinity stress, in vitro and in vivo, compared with results from previous reportsusing other corn genotypes under similar conditions.

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Analysis of Genetic Variability in Long-term Callus Cultures and Regenerated Plants of Maize.

Cited by 9 publications

References 27 publications

Twenty-Five Years of Propagation in Suspension Cell Culture Results in Substantial Alterations of the Arabidopsis Thaliana Genome

Twenty-Five Years of Propagation in Suspension Cell Culture Results in Substantial Alterations of the Arabidopsis Thaliana Genome

Regeneration and Analysis of Genetic Variability in Wild Sorghum, S. australiense Garber and Snyder.

NaCl tolerance in maize (Zea mays ssp. mays) x Tripsacum dactyloides L. hybrid calli in regenerated plants

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