Analysis of genome segments from six different isolates of bluetongue virus using RNA-RNA hybridisation: a generalised coding assignment for bluetongue viruses

Pedley, S.; Mohamed, Maizan; Mertens, Peter P. C.

doi:10.1016/0168-1702(88)90078-0

Cited by 31 publications

(24 citation statements)

References 31 publications

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“…A much better size estimate is obtained on agarose gels. It has been reported that all serotypes of BTV (Pedley et aL, 1988), EHDV (Brown et al, 1988) and other orbiviruses (Bodkin & Knudson, 1985;Gonzalez & Knudsom 1987 have similar dsRNA profiles on agarose gels. Similar results were obtained with the six EEV serotypes.…”

Section: Dot Blot Hybridization Of Eev Cascara-specific Dna Clonesmentioning

confidence: 99%

“…However, as shown by Pedley et al (1988) for BTV, the separation of dsRNA genome segments by PAGE is not a good reflection of the size of the respective RNA segments. A much better size estimate is obtained on agarose gels.…”

Section: Dot Blot Hybridization Of Eev Cascara-specific Dna Clonesmentioning

confidence: 99%

“…The EEV genome is composed of 10 genome segments and the P A G E separation profile of the segments differed considerably from those of BTV and AHSV. P A G E gels are, however, not suitable for estimating an accurate size for dsRNA segments (Pedley et al, 1988) so the different d s R N A isolates were also analysed on agarose gels. The separation of d s R N A segments from the six EEV serotypes on a 1 ~o agarose gel is shown in Fig.…”

Section: Characterization Of Eev Proteins and Dsrnamentioning

confidence: 99%

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The Characterization of Equine Encephalosis Virus and the Development of Genomic Probes

Viljoen

Huismans

1989

Journal of General Virology

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SUMMARYEquine encephalosis virus (EEV) is an orbivirus associated with a peracute illness of horses in southern Africa. The virus has now been partially purified for the first time and characterized on a molecular level. The virion is composed of 10 dsRNA segments and a protein capsid consisting of at least seven structural proteins that vary in M r from 36 000 to 120 000. Partial clones of six of the dsRNA segments of EEV serotype Cascara were obtained and analysed for possible use as serotype-specific or group-specific probes in the detection of EEV dsRNA. Cloned fragments of genome segments 3, 8 and 10 were found to show high conservation of these segments, hybridizing to dsRNA from the six EEV serotypes under conditions that indicated more than 90% sequence homology. The genome segment 2-specific probe did not hybridize with dsRNA from any of the other EEV serotypes, suggesting that this segment encodes the serotypespecific antigen of EEV. Cross-hybridization of probes from genome segments 3 and 5 with dsRNA from bluetongue virus (BTV), epizootic haemorrhagic disease virus (EHDV) and African horse sickness virus (AHSV) indicated that EEV is more closely related to BTV and EHDV than to AHSV. Both probes can be used to distinguish between EEV and AHSV dsRNA.

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Section: Dot Blot Hybridization Of Eev Cascara-specific Dna Clonesmentioning

confidence: 99%

Section: Dot Blot Hybridization Of Eev Cascara-specific Dna Clonesmentioning

confidence: 99%

Section: Characterization Of Eev Proteins and Dsrnamentioning

confidence: 99%

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The Characterization of Equine Encephalosis Virus and the Development of Genomic Probes

Viljoen

Huismans

1989

Journal of General Virology

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“…Studies with rotaviruses (Clarke & McCrae, 1982) and orbiviruses (Mertens et aL, 1987;Pedley et al, 1988) have shown that variations in the migration rates of genome segments during PAGE do not correlate with variations in near-terminal RNA sequence or in virus serotype, respectively. In addition, changes in the order of migration of individual genome segments of bluetongue virus have been observed with changes in polyacrylamide concentration (Mertens & Sangar, 1985).…”

Section: Introductionmentioning

confidence: 99%

Cytoplasmic Polyhedrosis Virus Classification by Electropherotype; Validation by Serological Analyses and Agarose Gel Electrophoresis

Mertens

Crook²,

Rubinstein

et al. 1989

Journal of General Virology

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SUMMARYSerological analyses of several different cytoplasmic polyhedrosis viruses (CPVs), including two type 1 CPVs from Bombyx mori, type 1 CPV from Dendrolimus spectabilis, type 12 CPV from Autographa gamma, type 2 CPV from Inachis io, type 5 CPV from Orgyia pseudotsugata and type 5 CPV from Heliothis armigera, demonstrated a close correlation between the antigenic properties of the polyhedrin or virus particle structural proteins and the genomic dsRNA electropherotypes. The dsRNAs of these viruses were analysed by electrophoresis in 3% and 10% polyacrylamide gels with a discontinuous Tris-HC1/Tris-glycine buffer system or by 1% agarose gel electrophoresis using a continuous Tris-acetate-EDTA buffer system. Electrophoretic analysis in agarose gels was found to be the most suitable for the classification of CPV isolates into electropherotypes, and the results obtained showed a close correlation with the observed antigenic relationships between different virus isolates. However, electrophoretic analysis in 10% polyacrylamide gels was most sensitive for the detection of intra-type variation and the presence of mixed virus isolates.

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“…2) similar, whereas the dsRNA profile of the European isolate was different. It has been shown that, in agarose gels, the mobilities of the dsRNA segments of bluetongue virus isolates are proportional to their Mrs and the RNA profile is common amongst all bluetongue virus isolates but is different from that seen in members of other orbivirus serogroups (Bodkin & Knudson, 1985;Kowlik & Li, 1987;Pedley et al, 1988). Therefore, our results indicate that all four isolates of aquatic rotaviruslike virus from North America have dsRNA segments of similar Mr whereas the isolate from Europe has dsRNA segments of different Mrs. Virion proteins of the five isolates were analysed in 12.5 ~ polyacrylamide gels (Laemmli, 1970).…”

mentioning

confidence: 99%

Heterogeneity in the Genome RNAs and Polypeptides of Five Members of a Novel Group of Rotavirus-like Viruses Isolated from Aquatic Animals

Samal¹,

Dopazo

Subramanian³

et al. 1991

Journal of General Virology

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Analysis of genome segments from six different isolates of bluetongue virus using RNA-RNA hybridisation: a generalised coding assignment for bluetongue viruses

Cited by 31 publications

References 31 publications

The Characterization of Equine Encephalosis Virus and the Development of Genomic Probes

The Characterization of Equine Encephalosis Virus and the Development of Genomic Probes

Cytoplasmic Polyhedrosis Virus Classification by Electropherotype; Validation by Serological Analyses and Agarose Gel Electrophoresis

Heterogeneity in the Genome RNAs and Polypeptides of Five Members of a Novel Group of Rotavirus-like Viruses Isolated from Aquatic Animals

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