Analysis of glutathione: implication in redox and detoxification

Pastore, Anna; Federici, Giorgio; Bertini, Enrico; Piemonte, Fiorella

doi:10.1016/s0009-8981(03)00200-6

Cited by 1,011 publications

(784 citation statements)

References 147 publications

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“…Kidneys are exposed to various cytotoxic agents before the elimination of these agents in urine. Thus the GSH concentrations in kidney cells are important (Pastore et al, 2003). In the present study, declined level of GSH was observed in gentamicin-induced rats when compared to control rats.…”

Section: Histopathological Observationsupporting

confidence: 54%

Protective Effect of Tecoma stans Flowers on Gentamicin-Induced Nephrotoxocity in Rats

Mo¹,

Anand²,

Priya³

2015

Asian J. of Biochemistry

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The present study was undertaken to evaluate the Ethanolic Extract of Tecoma stans (EETS) flowers for its protective effect on gentamicin-induced nephrotoxocity in rats. Nephrotoxocity was induced in rats by intraperitoneal administration of gentamicin 80 mg kgG 1 dayG 1 for seven days. Effect of concurrent administration of EETS at a dose of 500 mg kgG 1 dayG 1 given by intraperitoneal route was determined using serum and urinary creatinine and blood urea nitrogen as indicators of kidney damage. As nephrotoxocity of gentamicin is known to involve the induction of oxidative stress, so in vitro antioxidant activity, malondialdehyde (MDA) and glutathione (GSH) levels were also evaluated. Gentamicin-induced glomerular congestion and necrosis of kidney cells were found to be reduced in the group receiving EETS with gentamicin. Rats treated with gentamicin developed significant kidney dysfunction was observed from increased levels of urea, creatinine, sodium and decreased levels of protein, uric acid and potassium. In vitro studies revealed that the EETS posses significant antioxidant activity. The above results confirm that the flower extract acquire nephroprotective and regenerative capacity against gentamicin toxicity.

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Section: Histopathological Observationsupporting

confidence: 54%

Protective Effect of Tecoma stans Flowers on Gentamicin-Induced Nephrotoxocity in Rats

Mo¹,

Anand²,

Priya³

2015

Asian J. of Biochemistry

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“…This suggests that the addition of purified flavonoids to the diet could increase tissue and circulating total glutathione concentrations after prolonged administration and to some extent support the studies of Carlsen et al [24] in cultured HEP G2 cells. The increase in glutathione concentrations may be related to the role of this thiol in the detoxification of the electrophiles and free radicals; [25,26] these functions of glutathione have been recently reviewed by Pastore et al [27] Glutathione peroxidase is an antioxidant seleno-enzyme (found in most tissues) that reduces hydrogen peroxide and alkyl peroxide in tissues and uses glutathione as an essential redox substrate. Previous studies have shown that glutathione peroxidase activity is influenced by pesticide exposure.…”

Section: Discussionmentioning

confidence: 99%

Short- and long-term exposure to low levels of pesticide and flavonoid mixtures modify endogenous antioxidants in tissues of rats

Panemangalore

Bebe

2009

Journal of Environmental Science and Health, Part B

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The objective of this study was to determine if interaction between phytochemical mixtures and low level exposure to pesticides would modify tissue endogenous antioxidants in rats. Two experiments were conducted using dietary flavonoid (F) and oral pesticide (P) mixtures (FM, PM) on changes in the activities of superoxide dismutase (SOD), glutathione peroxidase (GPX) and glutathione concentrations (GSH) in male Sprague Dawley rats (150-175 g) fed for 2 or 4 weeks the AIN 93M diet with or without equal amounts of quercetin, rutin, catechin (FM) at 1.0 mM or 5 mM/kg diet, and with/without PM fed orally at 0.1 mL/d/5d/wk (PM = chlorpyrifos, endosulfan, thiram at 25% LD 50 in oil). Our data indicate that (compared to corresponding Control groups): (i) While, 2 weeks of feeding reduced liver and small intestinal mucosal (IM) SOD activity in the PFM1 (PM+FM1) group, 4 weeks of feeding increased only liver SOD activity in PM, FM1, FM5 and PFM1 and PFM5 groups; (ii) Liver and IM GSH levels increased in PFM groups after 2 or 4 weeks of exposure; plasma GSH increased in the groups fed FM5 with or without PM; (iii) Liver GPX activity declined in both 2 and 4 week experiments in the FM and PFM groups, respectively. These data suggests that metabolic interaction between FM and PM, and duration of exposure, can modify endogenous antioxidants. Data also underscore the prooxidant properties of flavonoids.

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“…Thus, a stable GSH concentration in the liver of silver carp in this study is assumed to result from an increase in GSH synthesis rather than from the cycling of GSSG to GSH. In general, an increase in the glutathione level of a cell makes it more resistant to antitumor agents, and also to radiation and oxidative pressures (Pastore et al, 2003). So, the high GSH level in the liver of silver carp may suggest a high ability to detoxify MCs and to release associated high oxidative pressure in phytoplanktivorous fish.…”

Section: Discussionmentioning

confidence: 99%

Sequential ultrastructural and biochemical changes induced in vivo by the hepatotoxic microcystins in liver of the phytoplanktivorous silver carp Hypophthalmichthys molitrix

Xie

Li³

et al. 2007

Comparative Biochemistry and Physiology Part C: Toxicology &

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Analysis of glutathione: implication in redox and detoxification

Cited by 1,011 publications

References 147 publications

Protective Effect of Tecoma stans Flowers on Gentamicin-Induced Nephrotoxocity in Rats

Protective Effect of Tecoma stans Flowers on Gentamicin-Induced Nephrotoxocity in Rats

Short- and long-term exposure to low levels of pesticide and flavonoid mixtures modify endogenous antioxidants in tissues of rats

Sequential ultrastructural and biochemical changes induced in vivo by the hepatotoxic microcystins in liver of the phytoplanktivorous silver carp Hypophthalmichthys molitrix

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