Analysis of gluten-hydrolyzing proteinase polymorphism in wheat grains damaged by Sunn Pest Eurygaster integriceps Put. and related bugs

Konarev, Alexander V.; Конарев, А. В.; Nefedova, L. I.; Gubareva, N. K.; Özay, Dilek Sivri

doi:10.3103/s1068367413060104

Cited by 4 publications

(2 citation statements)

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“…serve as an inexhaustible source of resistance genes for creating cultivars combining high yields and resistance to environmental factors. Evolutionary, Aegilops L. species are close to those of the genus Triticum L. (Dorofeev, 1971;Migushova, 1975;Konarev, 1980;Liu et al, 2015;Arora et al, 2017). However, the donors used in breeding programs, in most cases are characterized by the same resistance genes.…”

Section: Introductionmentioning

confidence: 99%

Metabolomic approach to search for fungal resistant forms of <i>Aegilops tauschii</i> Coss. from the VIR collection

et al. 2020

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Broadening of the genetic diversity of donors of resistance to biotic environmental factors is a challenging problem concerning Triticum L., which can be solved by using wild relatives of wheat, in particular, Aegilops tauschii Coss., in breeding programs. This species, believed to be the donor of D genome of common wheat (T. aestivum L.), is a source of some traits important for breeding. This greatly facilitates the possibility of crossing Ae. tauschii with common wheat. Aegilops L. species are donors of effective genes for resistance to fungal diseases in wheat. For instance, genes that determine resistance to rust agents in common wheat were successfully introgressed from Ae. tauschii into the genome of T. aestivum L. The aim of our study was to identify differences in metabolomic profiles of Ae. tauschii forms (genotypes), resistant or susceptible to such fungal pathogens as Puccinia triticina f. sp. tritici and Erysiphe graminis f. sp. tritici. These indicators may be used as biochemical markers of resistance. A comparative analysis of groups of Ae. tauschii accessions showed that metabolomic profiles of the forms with or without resistance to fungal pathogens differed significantly in the contents of nonproteinogenic amino acids, polyols, phytosterols, acylglycerols, mono- and oligosaccharides, glycosides, phenolic compounds (hydroquinone, kempferol), etc. This fact was consistent with the previously obtained data on the relationship between Fusarium resistance in oats (Avena sativa L.) and certain components of the metabolomic profile, such as acylglycerols, nonproteinogenic amino acids, galactinol, etc. Thus, our studies once again confirmed the possibility and effectiveness of the use of metabolomic analysis for screening the genetic diversity of accessions in the VIR collection, of Ae. tauschii in particular, in order to identify forms with a set of compounds in their metabolomic profile, which characterize them as resistant. Ae. tauschii accessions with a high content of pipecolic acids, acylglycerols, galactinol, stigmasterol, glycerol, azelaic and pyrogallic acids, campesterol, hydroquinone, etc., can be used for creating wheat and triticale cultivars with high resistance to fungal pathogens causing powdery mildew, brown rust, and yellow rust.

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Section: Introductionmentioning

confidence: 99%

Metabolomic approach to search for fungal resistant forms of <i>Aegilops tauschii</i> Coss. from the VIR collection

et al. 2020

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“…Thus, an infestation of Sunn pests ( E. integriceps , E. maura , and E. testudinaria ) might result in a 20–30% yield loss for barley and a 50–90% yield loss for wheat ( Gul et al 2006 ). Furthermore, it greatly reduces the baking quality of the flour due to gluten degradation by proteolytic enzymes ( Darkoh et al 2010 , Konarev et al 2013 ).…”

Section: Introductionmentioning

confidence: 99%

DNA barcoding and morphological analysis for rapid identification of most economically important crop-infesting Sunn pests belonging to Eurygaster Laporte, 1833 (Hemiptera, Scutelleridae)

Syromyatnikov¹,

Golub²,

Kokina³

et al. 2017

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The genus Eurygaster Laporte, 1833 includes ten species five of which inhabit the European part of Russia. The harmful species of the genus is E. integriceps. Eurygaster species identification based on the morphological traits is very difficult, while that of the species at the egg or larval stages is extremely difficult or impossible. Eurygaster integriceps, E. maura, and E. testudinaria differ only slightly between each other morphologically, E. maura and E. testudinaria being almost indiscernible. DNA barcoding based on COI sequences have shown that E. integriceps differs significantly from these closely related species, which enables its rapid and accurate identification. Based on COI nucleotide sequences, three species of Sunn pests, E. maura, E. testudinarius, E. dilaticollis, could not be differentiated from each other through DNA barcoding. The difference in the DNA sequences between the COI gene of E. integriceps and COI genes of E. maura and E. testudinarius was more than 4%. In the present study DNA barcoding of two Eurygaster species was performed for the first time on E. integriceps, the most dangerous pest in the genus, and E. dilaticollis that only inhabits natural ecosystems. The PCR-RFLP method was developed in this work for the rapid identification of E. integriceps.

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Gluten hydrolase gene silencing using RNAi and its effect on the Sunn pest growth and development

Amiri

Bandani

2020

Phytoparasitica

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Analysis of gluten-hydrolyzing proteinase polymorphism in wheat grains damaged by Sunn Pest Eurygaster integriceps Put. and related bugs

Cited by 4 publications

References 6 publications

Metabolomic approach to search for fungal resistant forms of <i>Aegilops tauschii</i> Coss. from the VIR collection

Metabolomic approach to search for fungal resistant forms of <i>Aegilops tauschii</i> Coss. from the VIR collection

DNA barcoding and morphological analysis for rapid identification of most economically important crop-infesting Sunn pests belonging to Eurygaster Laporte, 1833 (Hemiptera, Scutelleridae)

Gluten hydrolase gene silencing using RNAi and its effect on the Sunn pest growth and development

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