1999
DOI: 10.1002/(sici)1098-2795(199911)54:3<273::aid-mrd8>3.0.co;2-x
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Analysis of hamster protamines: Primary sequence and species distribution

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Cited by 12 publications
(14 citation statements)
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“…Consistent with previous data, the Ca/Mg protamine extraction procedure was not found to be as ef®cient in removing protamine 1 as protamine 2 compared to the DTT, MTAB, and GuCl treatment (Balhorn et al, 1990;Bellve et al, 1992Bellve et al, , 1993Corzett et al, 1999). However, the demonstration that protamines can be extracted from hamster sperm under conditions that appear to preserve a signi®cant fraction of the bound zinc con-®rms our hypothesis that the majority of the zinc we measure in sperm nuclei is actually bound to protamine.…”
Section: Discussionsupporting
confidence: 90%
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“…Consistent with previous data, the Ca/Mg protamine extraction procedure was not found to be as ef®cient in removing protamine 1 as protamine 2 compared to the DTT, MTAB, and GuCl treatment (Balhorn et al, 1990;Bellve et al, 1992Bellve et al, , 1993Corzett et al, 1999). However, the demonstration that protamines can be extracted from hamster sperm under conditions that appear to preserve a signi®cant fraction of the bound zinc con-®rms our hypothesis that the majority of the zinc we measure in sperm nuclei is actually bound to protamine.…”
Section: Discussionsupporting
confidence: 90%
“…For the Ca/Mg treatment protamine 1 comprised only 50% of the total extracted protein (Fig. 2), while it comprises 66% of the total protamine present in the hamster sperm head (Balhorn, 1989;Corzett, 1999). PIXE *The derived values of DNA mass, total protamine mass, protamine 2 content, and the ratio of total protamine mass to DNA mass in the nuclei of each species are also shown.…”
Section: Resultsmentioning
confidence: 97%
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