Expression of mammalian spermatozoal nucleoproteins

Dadoune, Jean‐Pierre

doi:10.1002/jemt.10317

Cited by 153 publications

(125 citation statements)

References 302 publications

(280 reference statements)

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“…Human spermatozoa are potentially more sensitive to ROS because of their relatively high residual content of histones reaching about 10 to 20 percent [Dadoune 2003;Wykes and Krawetz 2003] compared to animal models where about 1% of mouse sperm DNA remains bound to histones [van der Heijden et al 2006]. On an evolutionary basis, this may indicate an elevated transmission of epigenetic marks to offspring but this may produce a more vulnerable genome.…”

Section: Reactive Oxygen Speciesmentioning

confidence: 99%

“…Although some differences have been observed among mammals [Dadoune 2003;Oliva 2006], chromatin remodeling relies on a conserved sequence of events, that is, the incorporation of histone variants and covalent modifications of nucleosomal histones, such as acetylation and ubiquitination, the displacement of most histones by transition proteins (TPs), followed by the final deposition of protamines. Although little is known about this process, one may surmise that any alteration in this nuclear sequence of events is likely to have significant consequences on the genetic integrity of the gamete.…”

Section: Post-translational Modificationsmentioning

confidence: 99%

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Spermiogenesis and DNA Repair: A Possible Etiology of Human Infertility and Genetic Disorders

Leduc¹,

Nkoma²,

Boissonneault³

2008

Systems Biology in Reproductive Medicine

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This paper reviews the possible origin of sperm DNA fragmentation and focuses on the nuclear events associated with spermiogenesis as a potential source of genetic instability and reduced fertilizing potential of the mature male gamete. Recent findings suggest a programmed DNA fragmentation and DNA damage response during the chromatin remodeling steps in spermatids. We also discuss the spermatid DNA repair mechanisms and the possible involvement of condensing proteins, such as transition proteins and protamines, in the process, as this DNA fragmentation is normally not found in late spermatids. We propose that alterations in the chromatin remodeling steps or DNA repair in elongating spermatids may lead to persistent DNA breaks. This vulnerable step of spermiogenesis may provide a clue to the etiology of sperm DNA fragmentation associated with infertility in humans. This vulnerability is further emphasized given the haploid character of spermatids that must resolve programmed double-stranded breaks by an error-prone DNA repair mechanism. Therefore, spermiogenesis has probably been overlooked as an important source of genetic instability.

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Section: Reactive Oxygen Speciesmentioning

confidence: 99%

Section: Post-translational Modificationsmentioning

confidence: 99%

Spermiogenesis and DNA Repair: A Possible Etiology of Human Infertility and Genetic Disorders

Leduc¹,

Nkoma²,

Boissonneault³

2008

Systems Biology in Reproductive Medicine

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“…During spermatogenesis, sperm chromatin undergoes structural modifications to result in a highly condensed state [58] by the replacement of nucleo-histones by protamines [59,60]. Here we observe that normal histone packaging increases with an increase in NCS, and conversely, the proportion of sperm with abnormal histone increases with an increase in PCS.…”

Section: Discussionmentioning

confidence: 56%

Optimization of microelectrophoresis to select highly negatively charged sperm

Lee¹,

Murphy²,

Aston³

et al. 2016

J Assist Reprod Genet

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Purpose The sperm membrane undergoes extensive surface remodeling as it matures in the epididymis. During this process, the sperm is encapsulated in an extensive glycocalyx layer, which provides the membrane with its characteristic negative electrostatic charge. In this study, we develop a method of microelectrophoresis and standardize the protocol to isolate sperm with high negative membrane charge. Methods Under an electric field, the percentage of positively charged sperm (PCS), negatively charged sperm (NCS), and neutrally charged sperm was determined for each ejaculate prior to and following density gradient centrifugation (DGC), and evaluated for sperm DNA damage, and histone retention. Subsequently, PCS, NCS, and neutrally charged sperm were selected using an ICSI needle and directly analyzed for DNA damage. Results When raw semen was analyzed using microelectrophoresis, 94 % were NCS. In contrast, DGC completely or partially stripped the negative membrane charge from sperm resulting PCS and neutrally charged sperm, while the charged sperm populations are increased with an increase in electrophoretic current. Following DGC, high sperm DNA damage and abnormal histone retention were inversely correlated with percentage NCS and directly correlated with percentage PCS. NCS exhibited significantly lower DNA damage when compared with control (P < 0.05) and PCS (P < 0.05). When the charged sperm population was corrected for neutrally charged sperm, sperm DNA damage was strongly associated with NCS at a lower electrophoretic current. Conclusion The results suggest that selection of NCS at lower current may be an important biomarker to select healthy sperm for assisted reproductive treatment.

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“…The PT is composed of a variety of cytosolic and nuclear proteins (Dadoune, 2003;Longo et al, 1987;Meistrich, 1993;Oko and Sutovsky, 2009;Toshimori, 2003) which, together with the specific location of the theca, would suggest that it serves to bind the acrosome and post-acrosomal plasmalemma to the nucleus, thereby assisting in maintaining the shape of the sperm head (Longo and Cook, 1991). Accentuating this role of the PT is the observation that failure of the PT to form correctly results in the formation of acrosomeless round-headed human sperm (Escalier, 1990).…”

Section: The Perinuclear Theca (Pt)mentioning

confidence: 99%

“…Formation and shaping of the sperm head has been studied in numerous vertebrate and invertebrate species (Fawcett et al, 1971;Phillips, 1974;Toshimori and Ito, 2003), particularly in mammals, and a link established between defective head formation / structure and compromised fertility (Barth and Oko, 1989;Dadoune, 1995Dadoune, , 2003Zamboni, 1987;Zhuang et al, 2014). Although conflicting evidence has been presented in the literature, morphological data supplied by conventional transmission electron microscopy, supplemented by the use of animal models, have implicated various cellular components and processes in nuclear (head) morphogenesis (Fawcett, et al, 1971;Hermo et al, 2010;Kierszenbaum et al, 2003Kierszenbaum et al, , 2004Kierszenbaum et al, , 2007Meistrich, 1993).…”

Section: Introductionmentioning

confidence: 99%

Sperm head shaping in ratites: New insights, yet more questions

Plessis

Soley

2016

Tissue and Cell

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Highlights• Detailed description of the various factors involved in sperm head shaping.• First report on the presence of a perinuclear theca (PT) in birds.• The PT plays a role in stabilizing the sperm head during spermiogenesis in ratites.• The elaboration of the PT may be used to resolve phylogenetic questions. 2 AbstractHead shaping in mammalian sperm is regulated by a number of factors including acrosome formation, nuclear condensation and the action of the microtubular manchette. A role has also been suggested for the attendant Sertoli cells and the perinuclear theca (PT). In comparison, relatively little information is available on this topic in birds and the presence of a PT per se has not been described in this vertebrate order. This study revealed that a similar combination of factors contributed to head shaping in the ostrich, emu and rhea, although the Sertoli cells seem to play a limited role in ratites. A fibro-granular structure analogous to the mammalian PT was identified, consisting of sub-and post-acrosomal components. The latter was characterized by stage-specific finger-like projections that appeared to emanate from the cytoplasmic face of the nuclear envelope. They were particularly obvious beneath the base of the acrosome, and closely aligned, but not connected to, the manchette microtubules. During the final stages of chromatin condensation and elongation of the sperm head the projections abruptly disappeared. They appear to play a role in stabilizing the shape of the sperm head during the caudal translocation of the spermatid cytoplasm.

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Expression of mammalian spermatozoal nucleoproteins

Cited by 153 publications

References 302 publications

Spermiogenesis and DNA Repair: A Possible Etiology of Human Infertility and Genetic Disorders

Spermiogenesis and DNA Repair: A Possible Etiology of Human Infertility and Genetic Disorders

Optimization of microelectrophoresis to select highly negatively charged sperm

Sperm head shaping in ratites: New insights, yet more questions

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