Analysis of hepatitis B virus-mixed genotype infection by ultra deep pyrosequencing in Sudanese patients, 2015–2016

Enan, Khalid A; Minosse, Claudia; Hussein, Abdel Rahim Mohammed El; Selleri, Marina; Giombini, Emanuela; Capobianchi, Maria Rosaria; Elkhidir, Isam M; Mustafa, Mohamed O.; Khair, Osama M.; Hassan, Dina Ahamed; Garbuglia, Anna Rosa

doi:10.1007/s15010-019-01306-5

Cited by 5 publications

(1 citation statement)

References 39 publications

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“…The pattern of genotype distribution observed in this study is supported by several studies from Sudan [43,44] and Côte d'Ivoire [36] using the same research methods. For instance, genotype D is the dominant genotype in Northern Africa, Mediterranean and Middle East regions [29,45,46].…”

Section: Discussionsupporting

confidence: 87%

Genotyping and sero-virological characterisation of hepatitis B virus-infected blood donors in Central Eritrea

Hamida¹,

Raja²,

Petros³

et al. 2021

Preprint

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Aim To determine serological markers and genotypes profiles of HBV isolates in Central Eritrea. Materials & methods A total of 191 HBsAg sero-positive samples were randomly selected for the study from 23,232 screened blood donors from 2015 to 2017. Enzyme-linked immunosorbent assay was used to perform HBV serological markers screening, while genotypes were determined using type-specific primer-based multiplex-nested PCR. Results The median age of infected blood donors was 28.9 ± 10.26 years. Of 191 HBsAg reactive serum samples, 77.5% (148/191) were sero-positive for HBcAb-total, among which 99.3% (147/148) and 0.7% (1/148) were sero-positive for HBsAg and HBsAb, respectively. Interestingly, among 147 HBcAb-total/HBsAg reactive samples, 16 (10.9%) and 131 (77.9%) were sero-positive for HBeAg and HBeAb, respectively. For genotyping, 73 HBV isolates were successfully amplified and genotyped, with 59 (80.8%) had a mono-genotype and 14 (19.2%) had a mixed-genotype infection. Among HBV isolates with mono-genotypes: 39 (53.4%) D; 10 (13.7%) E; 6 (8.2%) A and 4 (5.5%) C. While five mixed-genotypes comprised: 6 (8.2%) C/D; 3 (4.1%) C/D/E; 2 (2.7%) each with genotype A/D and D/E; and 1 (1.4%) genotype B/D. Conclusion HBV genotype D is the predominant genotype, either as a HBV mono- or mixed-genotype infection, among blood donors in Eritrea.

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Section: Discussionsupporting

confidence: 87%

Genotyping and sero-virological characterisation of hepatitis B virus-infected blood donors in Central Eritrea

Hamida¹,

Raja²,

Petros³

et al. 2021

Preprint

View full text Add to dashboard Cite

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Genotype distribution of hepatitis B virus in a subset of infected young people in Central Nigeria

et al. 2019

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Long-term follow-up of a Japanese Patient With Hepatitis B Virus Genotype H and Human Immunodeficiency Virus Coinfection

et al. 2019

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Hepatitis B virus (HBV) genotypes affect the pathogenesis of disease progression during the course of HBV infection. In Japan, HBV genotype H is one of the rare HBV genotypes. We recovered HBV genotype H from a blood sample from a Japanese HIV-infected patient with acute exacerbation of chronic HBV infection. Due to the development of drugs for treating HBV and HIV, HBV genotype H and HIV coinfection has been well controlled by nucleos(t)ide analogs and highly active antiretroviral therapy, respectively, from 2002 to 2019. Further study is needed with regard to HBV genotype H and its pathogenesis.

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Analysis of hepatitis B virus-mixed genotype infection by ultra deep pyrosequencing in Sudanese patients, 2015–2016

Cited by 5 publications

References 39 publications

Genotyping and sero-virological characterisation of hepatitis B virus-infected blood donors in Central Eritrea

Genotyping and sero-virological characterisation of hepatitis B virus-infected blood donors in Central Eritrea

Genotype distribution of hepatitis B virus in a subset of infected young people in Central Nigeria

Long-term follow-up of a Japanese Patient With Hepatitis B Virus Genotype H and Human Immunodeficiency Virus Coinfection

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