Analysis of HSV isolated from patients with unilateral and bilateral herpetic keratitis

Tognon, Mauro; Manservigi, Roberto; Sebastiani, Adolfo; Bragliani, G; Busin, Massimo; Cassai, Enzo

doi:10.1007/bf00136456

Cited by 16 publications

(19 citation statements)

References 10 publications

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“…The culture medium was Eagle's minimal essential medium (E-MEM) (Gibco/BRL, Gaithersburg, MD) supplemented with heat-inactivated 10% fetal calf serum (FCS) (Gibco/BRL) and 1% antibioticeantimycotic solution (Zell Shield, Minerva Biolabs GmbH, Berlin, Germany). The neurovirulent strains LV [37] and MS (ATCC VR-540) of HSV-1 and HSV-2, respectively, were used for most in vitro studies and all in vivo experiments. Both strains were sensitive to Acyclovir (ACV).…”

Section: Cells and Virusesmentioning

confidence: 99%

The AGMA1 poly(amidoamine) inhibits the infectivity of herpes simplex virus in cell lines, in human cervicovaginal histocultures, and in vaginally infected mice

et al. 2016

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a b s t r a c tThe development of topical microbicides is a valid approach to protect the genital mucosa from sexually transmitted infections that cannot be contained with effective vaccination, like HSV and HIV infections. A suitable target of microbicides is the interaction between viral proteins and cell surface heparan sulfate proteoglycans (HSPGs). AGMA1 is a prevailingly cationic agmatine-containing polyamidoamine polymer previously shown to inhibit HSPGs dependent viruses, including HSV-1, HSV-2, and HPV-16. The aim of this study was to elucidate the mechanism of action of AGMA1 against HSV infection and assess its antiviral efficacy and biocompatibility in preclinical models. The results show AGMA1 to be a non-toxic inhibitor of HSV infectivity in cell cultures and human cervicovaginal histocultures. Moreover, it significantly reduced the burden of infection of HSV-2 genital infection in mice. The investigation of the mechanism of action revealed that AGMA1 reduces cells susceptibility to virus infection by binding to cell surface HSPGs thereby preventing HSV attachment. This study indicates that AGMA1 is a promising candidate for the development of a topical microbicide to prevent sexually transmitted HSV infections.

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Section: Cells and Virusesmentioning

confidence: 99%

The AGMA1 poly(amidoamine) inhibits the infectivity of herpes simplex virus in cell lines, in human cervicovaginal histocultures, and in vaginally infected mice

et al. 2016

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“…A neurovirulent strain of HSV-1 (strain LV) was used [26]. This variant was plaque-purified, grown on Vero cell monolayers (ATCC CCL81), and stored in aliquots at -80° until used.…”

Section: Virus and Viral Challengementioning

confidence: 99%

Mice Genetic Immunization with Plasmid DNA Encoding a Secreted Form of HSV-1 gB Induces a Protective Immune Response against Herpes simplex Virus Type 1 Infection

Caselli¹,

Grandi²,

Argnani

et al. 2001

Intervirology

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Intramuscularly (i.m.) delivered plasmid DNA encoding a secreted form of glycoprotein B of herpes simplex virus type 1 (HSV-1 gB1s) was evaluated for the ability to elicit a protective immune response in Balb/c mice. Animals received three i.m. injections of a gB1s expression plasmid (pRP-RSV-gB1s) or of a wild-type transmembrane gB1 coding plasmid (pRP-RSV-gB1), while control mice were injected with the vector alone (pRP-RSV). A specific antibody response was observed in almost all immunized animals, and in most cases antibodies were also detected after 1 month in the absence of further vaccine boosts. Serum antibodies mostly displayed neutralizing activity against HSV-1. Glycoprotein B1s DNA immunization was also effective in protecting animals against the primary infection induced by a subsequent HSV-1 challenge and limited HSV-1 infection of sensitive ganglia.

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“…The highly pathogenic morphologic variant of HSV-1 used in this study (LV) was isolated from a patient suffering from bilateral heipetic keratitis [22]. At the first in vitro passage 2 variants were selected in Veto cell monolayers: LV.…”

Section: Viruses and Cellsmentioning

confidence: 99%

“…For passive transfer experiments, antibodies directed against gBl-s were used, as this glycoprotein had previously shown a high immunogenicity in active immunization trials [8,11]. Rab bits were immunized by intramuscular injection of homolo gous polyclonal serum against gBl-s, given before, simulta neously or after the ocular challenge with a large, highly pathogenic, plaque variant of HSV-1 (large variant, LV) [22], In rabbits, this variant produces a stromal keratitis with neurological involvement, resulting in encephalitis and death of the animal [7]. The effects of passive immuni zation were compared with those obtained when rabbits were actively immunized through the injection of the re combinant gBl-s.…”

Section: Introductionmentioning

confidence: 99%

Effects of the Passive Transfer of Anti-gB Antibodies in a Rabbit Model of HSV-1 -Induced Keratitis

Incorvaia¹,

Manservigi

Parmeggiani³

et al. 1995

Ophthalmologica

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The effectiveness of passively transferred antibodies directed against the secretory form of herpes simplex virus type 1 (HSV-1) glycoprotein B (gB1-s) was tested in a rabbit model of ocular HSV-1 infection. The animals were passively immunized through the intramuscular injection of a homologous polyclonal anti-gB1-s antiserum at different times from the viral ocular challenge (i.e at ––24, 0, +24 and +48 h from infection). The effects observed in this trial were compared with those obtained in an active immunization trial, in which the animals were vaccinated with gBl-s before the ocular infection with HSV-1 (large variant). The results have shown that passive immunization appears quite effective in prophylactic utilization, whereas it is less effective when performed at 24 or 48 h after inoculation. By contrast, active immunization of rabbits proved to be highly effective both in preventing the development of fatal encephalitis and in reducing the severity of corneal lesions.

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Analysis of HSV isolated from patients with unilateral and bilateral herpetic keratitis

Cited by 16 publications

References 10 publications

The AGMA1 poly(amidoamine) inhibits the infectivity of herpes simplex virus in cell lines, in human cervicovaginal histocultures, and in vaginally infected mice

The AGMA1 poly(amidoamine) inhibits the infectivity of herpes simplex virus in cell lines, in human cervicovaginal histocultures, and in vaginally infected mice

Mice Genetic Immunization with Plasmid DNA Encoding a Secreted Form of HSV-1 gB Induces a Protective Immune Response against Herpes simplex Virus Type 1 Infection

Effects of the Passive Transfer of Anti-gB Antibodies in a Rabbit Model of HSV-1 -Induced Keratitis

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