Analysis of human herpes virus-6 genomes in lymphoid malignancy in Japan.

Sumiyoshi, Yoshiaki; Kikuchi, Masahiro; Ohshima, Kohichi; Takeshita, Masaaki; Eizuru, Yoshito; Minamishima, Y

doi:10.1136/jcp.46.12.1137

Cited by 18 publications

(18 citation statements)

References 8 publications

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“…The HHV-6 U22 gene was detected in 68 of the 86 patients (79.1%). Similar prevalences were found by others [Sumiyoshi et al, 1993;Krueger et al, 1994;Valente et al, 1996]. In contrast to EBV positive patients, the patients suffering from the nodular sclerosis type of Hodgkin's lymphoma were positive most frequently for HHV-6 (83.6%).…”

Section: Discussionsupporting

confidence: 89%

HHV‐6 and EBV DNA quantitation in lymph nodes of 86 patients with Hodgkin's lymphoma

Lacroix¹,

Jaccard²,

Rouzioux³

et al. 2007

Journal of Medical Virology

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Human herpesvirus (HHV-6) and Epstein-Barr virus (EBV), are two ubiquitous human herpesviruses which share many common features although they belong to different sub-families. In particular, both viruses are found in lymph nodes of patients suffering from Hodgkin's lymphoma. The aim of this study was to detect and to quantify independently HHV-6 and EBV by a real-time PCR in lymph nodes from 86 patients with Hodgkin's lymphoma. EBV quantitative method was compared with LMP-1 protein detection among the same samples. EBV genome was detected for 61.6% of the patients (53/86) and the highest prevalence of this virus was observed in Hodgkin's lymphoma with mixed-cellularity histopathological type (80%). In contrast to that, HHV-6 genome was detected for 79.1% of the patients (68/86) and was most observed in the nodular-sclerosis group (83.6%). Among the 68 HHV-6 positive samples, 63 belonged to the B subtype. A large number of biopsies (47.7%) were positive for both viruses whereas a little number (7%) was negative for both. EBV quantitation and LMP-1 immunohistochemistry were correlated statistically but this latter technique was less sensitive. Among the nodular-sclerosis patients, HHV-6-/EBV+ patients were significatively older than HHV-6+/EBV- patients. Patients infected dually had higher values of quantitation for each virus than those positive for one virus. Data of the clinical follow-up obtained by diagnosis and during the treatment of 83 patients, were correlated with the virological findings.

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Section: Discussionsupporting

confidence: 89%

HHV‐6 and EBV DNA quantitation in lymph nodes of 86 patients with Hodgkin's lymphoma

Lacroix¹,

Jaccard²,

Rouzioux³

et al. 2007

Journal of Medical Virology

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“…Our present large-scale study, using sensitive PCR analysis of 100 ng of lymph node tissue DNA, detected the HHV-6 genome in 35.1% of all HD patients. Minor differences between our results and those reported by others (10,40,43) probably reflect variations in the sensitivities of the various tests employed and in the amounts of DNA analyzed, as well as differences in the populations studied. By contrast, however, Shiramizu et al (39) found no HHV-6 sequences in a cohort of 47 pediatric HD patients.…”

Section: Discussioncontrasting

confidence: 55%

Real-Time PCR for Quantification of Human Herpesvirus 6 DNA from Lymph Nodes and Saliva

Collot¹,

Petit²,

Bron

et al. 2002

J Clin Microbiol

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A real-time quantitative PCR assay has been developed to measure human herpesvirus 6 (HHV-6) DNA in biological specimens. The assay sensitivity was 10 copies of DNA per well, with a linear dynamic range of 10 to 10 7 copies of HHV-6 DNA. Intra-and interassay variations were, respectively, 0.88 and 0.8% for samples containing 10 2 DNA copies, 0.99 and 0.96% for samples containing 10 4 copies, and 0.76 and 0.9% for samples containing 10 6 copies. Among 34 saliva samples from healthy subjects, 26 were found to contain HHV-6 DNA (76.5%; median, 23,870 copies/ml), and following a single freeze-thaw cycle, 25 of the same samples were found to be positive for HHV-6 DNA, although at a statistically significantly lower concentration (median, 3,497 copies/ml). The assay enabled detection of HHV-6 DNA in lymph node biopsies from patients with Hodgkin's disease (

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“…Since then, many studies aimed at identifying the HHV-6 genome in pathologic specimens using polymerase chain reaction (PCR) have been reported (Table 1). [28][29][30][31][32][33][34][35][36] Frequencies of positive HHV-6 DNA appear to vary widely among these studies, and may depend on the differences in PCR sensitivity for each study. Variant B has been identified more frequently than variant A.…”

Section: Hodgkin Lymphomamentioning

confidence: 99%

Human Herpesvirus 6 in Hematological Malignancies

Ogata

2009

J Clin Exp Hematopathol

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Pathogenetic roles of human herpesvirus (HHV)-6 in lymphoproliferative diseases have been of continued interest. Many molecular studies have tried to establish a pathogenic role for HHV-6 in lymphoid malignancies. However, whether HHV-6 plays a role in these pathologies remains unclear, as positive polymerase chain reaction results for HHV-6 in those studies may reflect latent infection or reactivation rather than presence of HHV-6 in neoplastic cells. A small number of studies have investigated HHV-6 antigen expression in pathologic specimens. As a result, the lack of HHV-6 antigen expression on neoplastic cells argues against any major pathogenic role of HHV-6. The role of HHV-6 in childhood acute lymphoblastic leukemia (ALL) has also been of interest but remains controversial, with 2 studies documenting higher levels of HHV-6 antibody in ALL patients, and another 2 large-scale studies finding no significant differences in HHV-6 seroprevalences between ALL patients and controls. Alternatively, HHV-6 is increasingly recognized as an important opportunistic pathogen. HHV-6 reactivation is common among recipients of allogeneic stem cell transplantation (SCT), and is linked to various clinical manifestations. In particular, HHV-6 encephalitis appears to be significant, life-threatening complication. Most HHV-6 encephalitis develops in patients receiving transplant from an unrelated donor, particularly cord blood, typically around the time of engraftment. Symptoms are characterized by short-term memory loss and seizures. Magnetic resonance imaging typically shows limbic encephalitis. Prognosis for HHV-6 encephalitis is poor, but appropriate prophylactic measures have not been established. Establishment of preventive strategies against HHV-6 encephalitis represents an important challenge for physicians involved with SCT.

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Analysis of human herpes virus-6 genomes in lymphoid malignancy in Japan.

Cited by 18 publications

References 8 publications

HHV‐6 and EBV DNA quantitation in lymph nodes of 86 patients with Hodgkin's lymphoma

HHV‐6 and EBV DNA quantitation in lymph nodes of 86 patients with Hodgkin's lymphoma

Real-Time PCR for Quantification of Human Herpesvirus 6 DNA from Lymph Nodes and Saliva

Human Herpesvirus 6 in Hematological Malignancies

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