Analysis of Human Spermatozoal Fertilizing Ability Using Zona-Free Ova

Rogers, B. Jane; Campen, Hana Van; Ueno, Marilynn S.; Lambert, Hovey; Bronson, Richard; Hale, Ralph W.

doi:10.1016/s0015-0282(16)44416-x

Cited by 285 publications

(89 citation statements)

References 21 publications

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“…Several laboratories have extensively evaluated the assay, and numerous modifications have been reported [14,42,44]. In fact, a major problem with the assay has been the failure of laboratories to use a single standard protocol, which results in difficulties in comparing and analyzing results.…”

Section: Assays Of Zona Binding and Oocyte Penetrationmentioning

confidence: 99%

Semen Analysis at the Turn of the Century: An Evaluation of Potential Uses of New Sperm Function Assays

Carrell¹

2000

Archives of Andrology

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Section: Assays Of Zona Binding and Oocyte Penetrationmentioning

confidence: 99%

Semen Analysis at the Turn of the Century: An Evaluation of Potential Uses of New Sperm Function Assays

Carrell¹

2000

Archives of Andrology

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“…The method used has been described previously [6]. Two drops of spermatozoa1 specimen, after incubation in BWW, were examined in the modified Coulter counter not mroe than 3 min after adding 20 cc of Isoton and 3 drops of Zaponin (Coulter Electronics Ltd.).…”

Section: Methodsmentioning

confidence: 99%

“…Rogers et al [6] determined the percentage of the human sperm penetration in fertile and infertile men. In the fertile group the penetration rate was 56% (range 14-loo%), but in the infertile group it was always less than 10% [6]. In this study the biological test is discussed in detail.…”

Section: Introductionmentioning

confidence: 99%

Human Spermatozoa Penetration to Zona-Free Hamster Ova and the Volume of Sperm Cells

May

Margaliot

Laufer

et al. 1986

Archives of Andrology

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Thirty-five infertile men were divided into two groups: those with normal spermograms (15 cases) and those with oligospennia (20 cases). Each semen sample was examined by its penetration into zona-free hamster ova and by an improved Coulter counter apparatus for measuring the volume of spermatozoal head. Both groups were further divided into two subgroups: those with normal (above 15%) and those with abnormal penetration assay (0-10%).The relationship between sperm modal size and sperm cell penetration into hamster ova in both groups-the oligospermic and the normospermic-was calculated and was found to be nonsignificant (correlation index: -0,154). It appears that the inability of some human sperm cells to penetrate the zona-free hamster ova cannot be attributed to the spermatozoal size.

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“…Two such sperm function tests; the Sperm Penetration Assay; SPA [46], and the Sperm DNA Decondensation (SDD TM ) Test [13,14], were developed and tested for capacity to predict ART outcome.…”

mentioning

confidence: 99%

“…After removal of the zona pellucida, hamster oocytes lose their species specificity with regards to human sperm [57]. Soon thereafter, these findings led to the development of a human male fertility test; the Sperm Penetration Assay (SPA) that was shown to have use in predicting the fertilization potential of human sperm to fertilize human oocytes [46]. The SPA evaluates aspects of the ability of human sperm to complete certain processes necessary to achieve fertilization using zona-free hamster oocytes.…”

mentioning

confidence: 99%

Evaluating a novel panel of sperm function tests for utility in predicting intracytoplasmic sperm injection (ICSI) outcome

Brown

Merryman²,

Rivnay

et al. 2013

J Assist Reprod Genet

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Purpose The objective of this study was to evaluate a panel of three sperm function tests; tests known to assess different aspects of sperm functionality and genomic integrity, the: 1) Sperm DNA Accelerated Decondensation (SDAD TM ) Test, 2) Sperm DNA Decondensation (SDD TM ) Test, and 3) Sperm Penetration Assay (SPA), determining if positive and negative test scores correlated with failed and successful ICSI outcomes, respectfully. Methods A prospective, double blinded, cohort study was performed. One study sample (ejaculated semen) was collected by each of the 60 male partners of the 60 couples enrolled in the study; males whose female partners were found to have no major female factor issues. The sperm from each male was analyzed in the SPA, and SDAD and SDD Tests, and used for ICSI (1 ICSI cycle per couple). Results The ICSI cycle pregnancy rate for this study was 50 %, with a delivery rate040 % (n060 ICSI cycles). The SPA and SDD Test scores did not significantly predict ICSI outcome when used as stand-alone tests (p>>0.05). However, when the SPA and SDD Test scores were used together, ICSI outcomes for a subgroup of 10 (16.7 %) males, were significantly predicted (p00.03), with 1 live birth, and 9 negatives where the transferred embryos did not implant. In total, 38.4 % of the couples in this study were found to have a very poor chance for a successful ICSI cycle. Conclusion SDAD Test scores alone, and SPA and SDD Test scores used together, significantly predicted failed ICSI outcomes. This indicates that the scores obtained when analyzing patients' sperm using a panel of sperm function tests; specifically, the SPA, and SDAD and SDD Tests, can be used to identify infertile couples who should not be directed to ICSI.

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Analysis of Human Spermatozoal Fertilizing Ability Using Zona-Free Ova

Cited by 285 publications

References 21 publications

Semen Analysis at the Turn of the Century: An Evaluation of Potential Uses of New Sperm Function Assays

Semen Analysis at the Turn of the Century: An Evaluation of Potential Uses of New Sperm Function Assays

Human Spermatozoa Penetration to Zona-Free Hamster Ova and the Volume of Sperm Cells

Evaluating a novel panel of sperm function tests for utility in predicting intracytoplasmic sperm injection (ICSI) outcome

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