Analysis of hybrid lethality in F1 wheat-rye hybrid embryos

Tikhenko, N.; Rutten, Twan; Voylokov, A. V.; Houben, Andreas

doi:10.1007/s10681-007-9528-x

Cited by 17 publications

(14 citation statements)

References 58 publications

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“…Rye lines L2 (and by inference, also the genetically related lines L3 and L564), as well as the unrelated line L535, carry the allele preventing the normal development of a hybrid embryo. This gene was named by Tikhenko et al (2005Tikhenko et al ( , 2008 Eml (embryo lethality). Because we cannot determine yet, whether the allele for embryo lethality is dominant or recessive, we designate the alleles determining the production of differentiated (normal or wild-type) and undifferentiated (lethal or mutant) embryos Eml-R1a and Eml-R1b, respectively, following the rules for gene symbolization (McIntosh et al 2008).…”

Section: Resultsmentioning

confidence: 99%

“…The hybrid seeds of all other lines were viable. The non-germinating hybrid seeds obtained from crosses with both L2 and L535 had a normal endosperm, but hybrid embryos were either absent, or varied in size from small to normal, but without any indication of tissue differentiation (Tikhenko et al 2005(Tikhenko et al , 2008. In the present paper, we describe the identification and mapping of the gene(s) responsible for the failure of these wheat × rye hybrid embryos to develop, i.e.…”

Section: Introductionmentioning

confidence: 94%

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Gene mutations in rye causing embryo lethality in hybrids with wheat: allelism and chromosomal localisation

Tikhenko¹,

Tsvetkova²,

Priyatkina³

et al. 2011

Biologia plant.

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In crosses between hexaploid wheat and inbred lines of rye, a small number of rye genotypes produce seeds carrying undifferentiated, non-viable embryos. Hybrids between such lines and those not showing this phenotype were used as pollen donors in crosses with bread wheat in order to determine the genetic basis of disturbed embryo development. A single gene, designated Eml-R1b, is causing this character. Molecular markers associated with F 2 genotypes derived from a contrasting rye inbred progeny were used for a linkage study. Recombinant inbred lines of an F 5 population served as testers. Eml-R1b maps to chromosome arm 6RL, along with two co-segregating microsatellite loci, Xgwm1103 and Xgwm732. Complementary interactions of deleterious genes in wheat and rye are discussed.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 94%

Gene mutations in rye causing embryo lethality in hybrids with wheat: allelism and chromosomal localisation

Tikhenko¹,

Tsvetkova²,

Priyatkina³

et al. 2011

Biologia plant.

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“…For Triticum aestivum 9 Secale cereale crosses, Tikhenko et al (2008) reported that embryos at 20 DAP responded better than those at 16 DAP. Working with Cicer arietinum 9 C. bijugum hybrids, Clarke et al (2006) proposed that the ideal time for embryo rescue was at the early globular stage (2-7 DAP) of embryogenesis, because at later stages (heart-torpedo; 15-20 DAP), the hybrid embryos were aborted and the response was drastically decreased.…”

Section: Discussionmentioning

confidence: 98%

Creation of novel interspecific-interploid Hylocereus hybrids (Cactaceae) via embryo rescue

2012

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In this study we developed a technique for rescuing embryos at a very early developmental stage following interspecific-interploid crosses between Hylocereus sp. Controlled hand pollinations were performed between the tetraploid H. megalanthus (Vaup.) Bauer as the female parent and either diploid H. monacanthus (Lem.) Britton et Rose or H. undatus (Haw.) Britton et Rose as the male parent. The fertilized ovules were excised from ovaries 10 or 30 days after pollination (DAP). Pollinated ovules containing the funiculi and placental tissue and immature embryos were placed on half-strength basal Murashige and Skoog (MS) medium containing 680 lM glutamine, 0.54 lM a-naphthaleneacetic acid, and 0.45 lM thidiazuron and supplemented with 0.00, 0.09, 0.17 or 0.26 M sucrose concentrations. The best ovule response was recorded at 30 DAP, and the most prolific callus formation was observed at 10 DAP. Callus formation was observed in most of the treatments using whole ovules but not in the isolated immature embryos. The calli were mucilaginous or compact, transparent and friable, but they did not form embryogenic structures. Embryo development was significantly affected by the sucrose concentration, and the best results were obtained with 0.17 M sucrose. More than 70 % of the obtained hybrids were successfully hardened off and transplanted in soil where they grew normally. Ploidy level analyses of 77 putative hybrids exposed diploid, triploid, tetraploid, and higher than tetraploid levels. Among those hybrids studied, 22 progenies were randomly chosen for amplified fragment length polymorphism analysis, and all were identified as genuine hybrids. The technology described here is an additional stage in the breeding program for Hylocereus species resulting in novel, interspecific hybrids obtained using the embryo rescue technique. Keywords Amplified fragment length polymorphism (AFLP) Á Flow cytometry Á a-Naphthaleneacetic acid Á Ovule culture Á Thidiazuron Á Vine cacti Abbreviations AFLP Amplified fragment length polymorphism BGU Ben-Gurion University of the Negev DAP Days after pollination IE Immature embryos MS Murashige and Skoog NAA a-Naphthaleneacetic acid NIB Nuclei isolation buffer TDZ Thidiazuron WO Whole ovules

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“…Embryo rescue is still being used in breeding programs for the generation of genetic variability and incorporation of desirable traits from inter-specific or inter-generic hybrids (for review see Sharma et al 1996). Embryo rescue to develop improved germplasm has been successfully used in legumes (Clarke et al 2006;Fratini and Ruiz 2006;Fratini and Ruiz 2008), cereals (Rodrangboon et al 2002;Tikhenko et al 2008), oilseed crops (Luhs and Friedt 1994;Muangprom et al 2006), horticultural crops Drew et al 2006;Yang et al 2007;Tian and Wang 2008) and many other economically important plant species. A more sophisticated approach to the creation of genetic variability was through somatic cell hybridization by fusion of protoplasts, and in the late 1970s several significant breakthroughs occurred in addition to publication of a number of reports pertaining to basic protoplast isolation and culture (for reviews see Lynch et al 1993;Waara and Glimelius 1995;Davey et al 2005;Liu et al 2005).…”

Section: Technology-based Breedingmentioning

confidence: 99%

Functional Genomics For Crop Improvement

Ganeshan

Sharma

Chibbar

2009

Molecular Techniques in Crop Improvement

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Plant breeding has had a tremendous influence on crop improvement. However, due to dwindling germplasm resources, identification of variability for incorporation into new cultivars is becoming more difficult. Therefore, there has been recourse to alternative approaches including mutagenesis, tissue culture and genetic transformation to aid breeding programs. Furthermore, with the vast repertoire of genome-wide data from different expression profiling techniques such as microarrays, more subtle understanding of gene expression is being obtained and is further helping plant breeders to entertain a different selection approach based on expression quantitative traits to maximize combinations of genes capable of conferring high performance. In this chapter, we review some of the aspects of plant breeding and the influence functional genomics has on breeding programs. Some of the challenges to functional genomics and breeding come from establishment of high-throughput transformation systems to assess gene function, which is limiting functional characterization of numerous genes in their respective crops. Therefore, this chapter also focuses on the need to gain better understanding of the development of gene transfer systems for crop plants to make use of the array of available gene information data.

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Analysis of hybrid lethality in F1 wheat-rye hybrid embryos

Cited by 17 publications

References 58 publications

Gene mutations in rye causing embryo lethality in hybrids with wheat: allelism and chromosomal localisation

Gene mutations in rye causing embryo lethality in hybrids with wheat: allelism and chromosomal localisation

Creation of novel interspecific-interploid Hylocereus hybrids (Cactaceae) via embryo rescue

Functional Genomics For Crop Improvement

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