Analysis of immediate changes of water-soluble metabolites in alkali-burned rabbit cornea, aqueous humor and lens by high-resolution 1H-NMR spectroscopy

Abstract

The present study provides a valuable contribution to the knowledge of metabolic alterations in alkali-burned eyes. It shows that 1H-NMR spectroscopy is well suited for simultaneous qualitative and quantitative analysis of changes of metabolite concentrations in damaged tissues. This can help us to better evaluate and understand the biological alterations due to alkali burn.

High-resolution magic angle spinning 1H NMR spectroscopy of metabolic changes in rabbit lens after treatment with dexamethasone combined with UVB exposure

“…The major advantage of the HR-MAS method is that high-quality spectra can be obtained from intact tissue samples. In contrast to previous reports on NMR spectroscopy of lens extracts from our laboratory and others [13,28,36], labour-intensive and tissue-destructive extraction methods are avoided. Tissue extractions involve possible oxidation or hydrolysis of substances, and volatile compounds might be lost.…”

Time dependency of metabolic changes in rat lens after in vivo UVB irradiation analysed by HR-MAS 1H NMR spectroscopy

“…The HR-MAS 1 H NMR spectra obtained from intact rat lenses were of high resolution quality comparable to those obtained in previous experiments with intact lens tissues (Risa et al, 2004) and tissue extracts (Risa et al, 2002). A representative reduced NMR spectrum (bucket width 0.001 ppm) of a control lens with peak assignments of more than 25 different metabolites is presented in Fig.…”

“…The detailed information on the metabolic composition was comparable to that acquired by NMR spectroscopy of lens tissue extracts (Midelfart et al, 1996;Risa et al, 2002). In the present study broad resonances from macromolecules and lipids with low mobility and hence short transverse relaxation time (T 2 ) were suppressed by using a one-dimensional T 2 -filtered pulse sequence with an effective spin-echo delay of 144 msec.…”

“…Phosphorus-31 and proton NMR are the most common nuclei used in biological investigations. Several studies using 31 P or 1 H NMR spectroscopy have been performed on lens extracts (Meneses et al, 1990;Greiner et al, 1994;Midelfart et al, 1996;Risa et al, 2002).…”

Effect of UVA and UVB Irradiation on the Metabolic Profile of Rabbit Cornea and Lens Analysed by HR-MAS ¹H NMR Spectroscopy

“…3 mm) was cut out from the middle of the cornea (including all anatomic layers) and the average weight of the samples of corneas was 19.1 8 7.1 mg. H Cosy) and J resolved spectra were recorded in order to assign complicated coupling patterns. Peak assignments were performed according to previous reports [21,22,[26][27][28][29] .…”