Analysis of Immunological Activity of Recombinant Outer Membrane Protein of Fusobacterium Necrophorum

Abstract

To investigate the prokaryotic expression of 93 ku outer membrane protein (OMP) obtained from Fusobacterium necrophorum and the response to immunization and cytotoxicity in mice. The recombinant plasmid pET-28a-OMP was transformed into E. coli BL21 (DE3) competent form, and a recombinant outer membrane protein containing 2 His tags (label size 3.8 ku) was expressed. The mice were immunized as immunization antigens by Freund’s incomplete adjuvant. SDS-PAGE and Western blot analysis showed that the recombinant protein had a molecular weight of about 93 ku. After the immunization, the serum antibody titer of the experimental group was higher, at the same time, in the challenge experiment, the liver has certain toxicity, and the protection rate is 83.3%. The recombinant 93 ku outer membrane protein of Fusobacterium necrophorum has a good immune response level and provides basic data for the study of the subunit vaccine of Fusobacterium necrophorum.