There are two major problems in the evaluation of the teaching quality of English writing: the weak logic of the evaluation system and the low reliability of the evaluation model. To solve the problems, this paper put forward an evaluation method for the teaching quality of English writing based on the analytical hierarchy process (AHP). Firstly, the authors reviewed the current evaluation methods for the teaching quality of English writing. Next, hierarchical evaluation systems were established for the teaching quality of English writing from the perspectives of teachers and students, respectively. After that, the AHP method and the grey theory were introduced to set up an evaluation model for the teaching quality of English writing. Finally, several strategies were presented to improve the teaching quality of English writing. The proposed evaluation systems and model enriched the theories on teaching quality evaluation of English writing, and promoted the teaching quality of English writing.
To investigate the prokaryotic expression of 93 ku outer membrane protein (OMP) obtained from Fusobacterium necrophorum and the response to immunization and cytotoxicity in mice. The recombinant plasmid pET-28a-OMP was transformed into E. coli BL21 (DE3) competent form, and a recombinant outer membrane protein containing 2 His tags (label size 3.8 ku) was expressed. The mice were immunized as immunization antigens by Freund’s incomplete adjuvant. SDS-PAGE and Western blot analysis showed that the recombinant protein had a molecular weight of about 93 ku. After the immunization, the serum antibody titer of the experimental group was higher, at the same time, in the challenge experiment, the liver has certain toxicity, and the protection rate is 83.3%. The recombinant 93 ku outer membrane protein of Fusobacterium necrophorum has a good immune response level and provides basic data for the study of the subunit vaccine of Fusobacterium necrophorum.
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