Analysis of interaction between sulfated polysaccharides and HIV oligopeptides by surface plasmon resonance

Battulga, Tungalag; Tumurbaatar, Oyunjargal; Ganzorig, Oyundelger; Ishimura, Takahisa; Kanamoto, Taisei; Nakashima, Hideki; Miyazaki, Kensuke; Yoshida, Takashi

doi:10.1016/j.ijbiomac.2018.12.010

Cited by 28 publications

(15 citation statements)

References 14 publications

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“…The cells and the virus were treated with the indicated samples at different stages, and then the expression of the ALV-J p27 antigen was detected by ELISA. Studies have found that many polyanions, such as carrageenan [ 56 , 57 , 58 ], heparin [ 59 ], ulvan [ 60 , 61 ] and dextran sulfate [ 62 ], could inhibit virus adsorption onto host cells. Similar to our previous studies, the SFP played a role only in the virus adsorption stage ( Figure 7 b).…”

Section: Resultsmentioning

confidence: 99%

Preparation of New Sargassum fusiforme Polysaccharide Long-Chain Alkyl Group Nanomicelles and Their Antiviral Properties against ALV-J

et al. 2021

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Avian leukosis virus subgroup J (ALV-J) is an immunosuppressive virus which has caused heavy losses to the poultry breeding industry. Currently, there is no effective medicine to treat this virus. In our previous experiments, the low-molecular-weight Sargassum fusiforme polysaccharide (SFP) was proven to possess antiviral activity against ALV-J, but its function was limited to the virus adsorption stage. In order to improve the antiviral activity of the SFP, in this study, three new SFP long-chain alkyl group nanomicelles (SFP-C12M, SFP-C14M and SFP-C16M) were prepared. The nanomicelles were characterized according to their physical and chemical properties. The nanomicelles were characterized by particle size, zeta potential, polydispersity index, critical micelle concentration and morphology. The results showed the particle sizes of the three nanomicelles were all approximately 200 nm and SFP-C14M and SFP-C16M were more stable than SFP-C12M. The newly prepared nanomicelles exhibited a better anti-ALV-J activity than the SFP, with SFP-C16M exhibiting the best antiviral effects in both the virus adsorption stage and the replication stage. The results of the giant unilamellar vesicle exposure experiment demonstrated that the new virucidal effect of the nanomicelles might be caused by damage to the phospholipid membrane of ALV-J. This study provides a potential idea for ALV-J prevention and development of other antiviral drugs.

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Section: Resultsmentioning

confidence: 99%

Preparation of New Sargassum fusiforme Polysaccharide Long-Chain Alkyl Group Nanomicelles and Their Antiviral Properties against ALV-J

et al. 2021

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“…Currently, some studies were conducted to evaluate the interaction of polysaccharides with proteins through SPR ( Thompson and Dalgleish, 2010 ; Gao et al., 2013 ; Wang et al., 2019 ). For sulfated polysaccharides, the binding affinity may be associated with sulfated degree ( Battulga et al., 2019 ). The polysaccharides from the ink of cuttlefish Sepiella maindroni de Rochebruns showed a reduced binding affinity toward EGFR, when the sulfated degree decreased from 34.7% to 8.2% ( Jiang et al., 2018 ).…”

Section: Discussionmentioning

confidence: 99%

“…The antiviral mechanism of sulfated polysaccharides attributed to prevention of virus entry, which is mediated by the interactions of anionic charges in sulfated polysaccharides with positively charged domains of viral envelope glycoproteins, leading to shielding of the functional domain involved in virus attachment to cell surface receptors ( Damonte et al., 2004 ; Pujol et al., 2007 ). Recently, it has been demonstrated that the anti-HIV activity of sulfated polysaccharides attributed to interaction between the negatively charged sulfate groups and positively charged amino acid regions in the HIV gp120 ( Battulga et al., 2019 ). However, little study was conducted to identify which positively charged residues were interacted with sulfated polysaccharides.…”

Section: Introductionmentioning

confidence: 99%

Identification of the amino acids residues involved in hemagglutinin-neuraminidase of Newcastle disease virus binding to sulfated Chuanmingshen violaceum polysaccharides

Song

Liu

et al. 2021

Poultry Science

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The antiviral mechanism of sulfated polysaccharides is supposed to prevent virus entry, which is mediated by the interactions of anionic charges on sulfated polysaccharides with positively charged domains of viral envelope glycoproteins, leading to shielding of the functional domain involved in virus attachment to cell surface receptors. But, few direct evidences were reported. In the previous study, we found that sulfated Chuanmingshen violaceum polysaccharides ( sCVPS ) possessed remarkable inhibitory effect against Newcastle disease virus ( NDV ) through inhibition of NDV attachment to host cells. Whether sCVPS bound to hemagglutinin-neuraminidase ( HN ) leading to inhibition of NDV attachment needs to be further clarified. The present study conducted site-directed mutagenesis of possible positively charged residues of HN, and found that mutants R197G, H199G, R363G, and R523G could significantly decrease the inhibitory effects of sCVPS on receptor binding ability through hemadsorption assay, especially R363G which suggested that binding to R363 is more effective to shield the sialic acid binding sites. Dual mutants (R363G/R197G, R363G/H199G and R363G/R523G) induced more decreased inhibitory effect of sCVPS than single mutants. The immunofluorescence study using FITC-labeled sCVPS found that the fluorescence intensity of mutants R363G and R363G/H199G were significantly decreased. The binding kinetics of sCVPS to HN measured by surface plasmon resonance indicated that sCVPS had a higher binding affinity for wild-type HN than mutants R363G and R363G/H199G. Plaque reduction study was performed using recombinant NDV with mutant HN R363G and HN R363G/H199G , which showed significantly decreased inhibitory effects of sCVPS against mutant NDV adsorption to BHK-21 cells. These results suggested that the residues R197, H199, R363, and R523 were the binding sites for sCVPS, especially R363 act as the main interaction site. The present study provided direct evidence for the theory that antiviral mechanism of sulfated polysaccharides attributed to anionic groups binding to the positively charged residues of viral proteins which led to the shielding of receptor binding sites.

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“…New drugs must be developed with more potent bioactivities, less toxicity, and different mechanisms of action, especially by using raw materials that are widely available. The sulfated polysaccharides have such properties; they provide not only antiviral but also anti‐inflammatory, antioxidant, immunomodulatory and other benefits [2–3] …”

Section: Introductionmentioning

confidence: 99%

Chemically Sulfated Polysaccharides from Agaricus blazei Murill: Synthesis, Characterization and Anti‐HIV Activity

Zhao¹,

Tian

Wang³

et al. 2021

Chemistry & Biodiversity

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AIDS, caused by HIV‐1, is one of the most dangerous infectious diseases in the world. Therefore, it is necessary to develop new drugs with more potent bioactivities, less toxicity and higher tolerability for controlling the viral load, particularly by using the raw materials that are widely available. Agaricus blazei Murill (AbM), known in China as jisongrong, is of great importance as a food source and as a health‐promoting supplement for immunomodulation. The polysaccharides of AbM exhibit various biological activities, such as regulating cellular immunity and providing anti‐oxidative, anti‐infective, and anti‐inflammatory effects. At present, to our knowledge, no report has explored the chemically sulfated and anti‐HIV‐1 activity of AbM polysaccharides. Herein, the sulfated AbM polysaccharides with different sulfur contents were prepared by the chlorosulfonic acid‐pyridine method. The characteristics of sulfated derivatives were established by the determination of the sulfur content, the relative molecular weight, and the Fourier‐transform infrared spectroscopy. The anti‐HIV activities of the sulfated AbM polysaccharides were evaluated by CCK‐8 and the single‐cycle pseudovirus infection (TZM‐bl) assay. The sulfated AbM polysaccharides had strong antiviral properties, and the half‐maximal inhibitory concentrations approached that of the positive control, azidothymidine. Sulfated modification of AbM polysaccharides can increase their anti‐HIV pharmacological activity, which makes them promising alternative candidates as bioactive macromolecules for biomedical applications in HIV/AIDS.

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Analysis of interaction between sulfated polysaccharides and HIV oligopeptides by surface plasmon resonance

Cited by 28 publications

References 14 publications

Preparation of New Sargassum fusiforme Polysaccharide Long-Chain Alkyl Group Nanomicelles and Their Antiviral Properties against ALV-J

Preparation of New Sargassum fusiforme Polysaccharide Long-Chain Alkyl Group Nanomicelles and Their Antiviral Properties against ALV-J

Identification of the amino acids residues involved in hemagglutinin-neuraminidase of Newcastle disease virus binding to sulfated Chuanmingshen violaceum polysaccharides

Chemically Sulfated Polysaccharides from Agaricus blazei Murill: Synthesis, Characterization and Anti‐HIV Activity

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