Analysis of intermediates from polycyclic aromatic hydrocarbon biodegradation

Copper, Christine L.

doi:10.1002/jssc.200301615

Cited by 5 publications

(4 citation statements)

References 14 publications

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“…Upon background correction with ALS, the LODs via SPE-RTF meet the expectations for the analysis of B[a]P metabolites in urine samples[36][37][38][39][40][41][42][43][44][45].…”

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confidence: 58%

“…Analysis of metabolites has been accomplished via high-performance liquid chromatography (HPLC), capillary electrophoresis (CE) and gas chromatography (GC). Ultraviolet-visible absorption and RTF detection have been widely used with both HPLC and CE [40][41][42][43][44][45]. The ultimate specificity belongs to high-resolution MS either coupled to gas chromatography (GC-MS) or HPLC (HPLC-MS) [46][47][48][49][50][51].…”

Section: Resultsmentioning

confidence: 99%

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Room temperature fluorescence spectroscopy of benzo[a]pyrene metabolites on octadecyl extraction membranes

Alfarhani

Al-Tameemi

Schenone

et al. 2016

Microchemical Journal

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a b s t r a c tBenzo[a]pyrene (B[a]P) is a prototypic carcinogenic polycyclic aromatic hydrocarbon (PAH), which requires metabolic activation to produce its detrimental effects. Measurement of B[a]P metabolites in human urine could provide a direct way to assess individual differences in susceptibility to PAH-related cancer. This article focuses on the development of screening methodology for the routine analysis of B[a]P metabolites in urine samples. It explores the solid-surface room-temperature fluorescence (RTF) properties of 3-hydroxybenzo[a]pyrene, benzo[a]pyrene-trans-9,10-dihydrodiol, benzo[a]pyrene-r-7,t-8,c-9-tetrahydrotriol and benzo[a]pyrene-r-7,t-8,c-9,c-10-tetrahydrotetrol previously extracted from urine samples with octadecyl-silica membranes. Relative standard deviations varying from 2.1% (benzo[a]pyrene-r-7,t-8,c-9-tetrahydrotriol) to 8.6% (3-hydroxy-benzo[a]pyrene) are obtained with the aid of fiber optic probe that eliminates the need for manual optimization of signal intensities. Analytical recoveries from human urine samples varied from 87.5 ± 3.1% (3-hydroxy-benzo[a]pyrene) to 99.8 ± 2.5% (benzo[a]pyrene-r-7,t-8,c-9,c-10-tetrahydrotetrol). The excellent analytical figures of merit and the simplicity of the experimental procedure demonstrate the potential of this approach for screening biomarkers of PAH exposure in numerous urine samples.

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“…Upon background correction with ALS, the LODs via SPE-RTF meet the expectations for the analysis of B[a]P metabolites in urine samples[36][37][38][39][40][41][42][43][44][45].…”

mentioning

confidence: 58%

Section: Resultsmentioning

confidence: 99%

Room temperature fluorescence spectroscopy of benzo[a]pyrene metabolites on octadecyl extraction membranes

Alfarhani

Al-Tameemi

Schenone

et al. 2016

Microchemical Journal

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“…A variety of instrumental methods of analysis for PAHs are routinely used, including HPLC coupled with fluorescence detection, GC-MS, GC-MS/MS, and LC-MS/MS [16][17][18][19][20][21]. Although detection itself in most cases is straightforward, reliable sample preparation is not.…”

Section: Introductionmentioning

confidence: 99%

Rapid quick, easy, cheap, effective, rugged, and safe extraction with novel phospholipid cleanup: A streamlined ultra high performance liquid chromatography with ultraviolet detection approach for screening polycyclic aromatic hydrocarbons in avian blood cells and plasma

Provatas

Yevdokimov

King

et al. 2015

J of Separation Science

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A streamlined method has been developed for the isolation and analysis of polycyclic aromatic hydrocarbons in avian blood cells and plasma utilizing quick, easy, cheap, effective, rugged, and safe extraction in combination with novel phospholipid cleanup technology. A variety of traditional extraction and cleanup techniques have been employed in the preparation and analysis of polycyclic aromatic hydrocarbonsin a variety of matrices; liquid-liquid partitioning, solid-phase extractions, gel permeation chromatography, and column chromatography are all effective techniques, however they are laborious and time consuming processes that require large amounts of solvent. Using quick, easy, cheap, effective, rugged, and safe extraction coupled with phospholipid cleanup, samples can be quickly screened while maintaining high throughput and sensitivity. With a liquid chromatography approach, analysis times may be kept short at 16 min while maintaining high analyte recovery. Recoveries in quality control samples ranged from 70 to 109%, with average surrogate recoveries of 80.6 ± 1.10%. The result of using a quick, easy, cheap, effective, rugged, and safe extraction approach in conjunction with phospholipid cleanup is a methodology that significantly reduces sample preparation time and solvent use while maintaining high sensitivity and reproducibility.

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“…Thereafter, the raw analyte is usually cleaned up and separated into different polarity groups, with solid‐phase extraction or column chromatography using, for example, silica gel and different solvent combinations. After extraction and clean‐up/fractionation, the analytes are quantified by HPLC/DAD or GC with flame ionization or mass spectrometric detectors (Meyer et al, 1999; Lundstedt et al, 2006a), capillary electrophoresis (Copper, 2003), and liquid chromatography–mass spectrometry (Galceran and Moyano, 1996; Letzel et al, 2001; Lintelmann et al, 2006; Delhomme et al, 2008). Some problems that have been encountered in past analyses of OPAHs include low recoveries, matrix interferences, complicated and time‐consuming sample preparation steps, the high cost of analysis, a shortage of authentic reference/labeled standards, and a lack of certified reference materials (Meyer et al, 1999; Albinet et al, 2006; Lundstedt et al, 2006a; Lundstedt et al, 2007).…”

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confidence: 99%

Analysis of Polycyclic Aromatic Hydrocarbons and Their Oxygen‐Containing Derivatives and Metabolites in Soils

Bandowe

Wilcke

2010

J of Env Quality

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Although polycyclic aromatic hydrocarbons (PAHs) have been extensively studied, the knowledge of their oxygen-containing derivatives and metabolites (OPAHs) in soils is limited. We modified and tested an existing analytical protocol involving pressurized liquid extraction of soil followed by fractionation of target compounds into PAHs and OPAHs on a silica gel column and gas chromatography/ mass spectrometry-based separation and quantification. Polycyclic aromatic hydrocarbons and carbonyl-OPAHs were quantified directly after separation on silica gel columns, and hydroxyl/carboxyl-OPAHs were quantified after silylation with N,O-bis(trimethylsilyl)trifluoroacetamide. Recoveries between 78 and 97% (relative standard deviation [RSD], 5-12%) were obtained for six carbonyl-OPAHs, whereas 1,2-acenaphthenequinone and 1,4-naphthoquinone showed lower recoveries of 34 and 44% (RSD, 19 and 28%, respectively). Five hydroxyl/carboxyl-OPAHs had recoveries between 36 and 70% (RSD, 13-46%), six others had between 2 and 7% (RSD, 8-25%), and nine were lost in sample preparation. Limits of detection ranged from 0.1 to 1.6 ng g(-1) for OPAHs and from 0.01 to 0.56 ng g(-1) for PAHs. The protocol was applied to soils from a former gasworks site, Berlin, an urban soil from Mainz, both in Germany, and a forest soil from near Manaus, Brazil. The sums of 34 PAH concentrations were 107,000, 3505, and 21 ng g(-1); those of seven carbonyl-OPAHs were 15,690, 170, and 7 ng g(-1); and those of 11 hydroxyl/carboxyl-OPAHs 518, 36, and 16 ng g(-1) for Berlin, Mainz, and Manaus soils, respectively. Several OPAHs were present at concentrations higher than or equal to their parent PAHs, demonstrating the importance of OPAH measurement for the assessment of PAH-related environmental risks.

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Analysis of intermediates from polycyclic aromatic hydrocarbon biodegradation

Cited by 5 publications

References 14 publications

Room temperature fluorescence spectroscopy of benzo[a]pyrene metabolites on octadecyl extraction membranes

Room temperature fluorescence spectroscopy of benzo[a]pyrene metabolites on octadecyl extraction membranes

Rapid quick, easy, cheap, effective, rugged, and safe extraction with novel phospholipid cleanup: A streamlined ultra high performance liquid chromatography with ultraviolet detection approach for screening polycyclic aromatic hydrocarbons in avian blood cells and plasma

Analysis of Polycyclic Aromatic Hydrocarbons and Their Oxygen‐Containing Derivatives and Metabolites in Soils

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