Analysis of iron-binding components in the low molecular weight fraction of rat reticulocyte cytosol

Bakkeren, Dirk L; Jeu-Jaspars, C.M.H. de; Heul, C. Van Der; Eijk, H.G. Van

doi:10.1016/0020-711x(85)90177-6

Cited by 66 publications

(17 citation statements)

References 16 publications

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“…[59] (b) The other solution gives values at very low temperatures: (12) This model predicts a parabolic increase in the critical temperature when the external magnetic field increases. However, this effect should be small since aЈ is the same coefficient as in Equation (11). In our experiments, however, no such phenomena were observed.…”

Section: R[xln(2scontrasting

confidence: 64%

High‐Field EPR Study of Frozen Aqueous Solutions of Iron(III) Citrate Complexes

et al. 2005

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Keywords: High-field EPR spectroscopy / Iron / Coordination chemistry / Citrate ligand / Tripodal ligand / Spin crossover High-field electron paramagnetic resonance spectra have been measured for frozen aqueous solutions of Fe III citrate at acidic and neutral pH values. At high fields (up to 12 T) and high frequencies (95, 190 and 285 GHz), fine-structure peaks due to resonance between Kramers doublets (±1/2, ±3/2, ±5/ 2) of an S = 5/2 species can be observed when the experiments are performed at high temperatures. A full-matrix diagonalisation approach has been used to derive the spinHamiltonian parameters for the S = 5/2 spin state. In acidic media (pH = 2), the spacing of the fine structure reveals that the value of the axial zero-field splitting (ZFS) parameter D is −0.12 cm −1 without rhombicity. A small but significant g anisotropy can be observed which depends slightly on the frequency and the temperature (g x = 2.014, g y = 2.014 and

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Section: R[xln(2scontrasting

confidence: 64%

High‐Field EPR Study of Frozen Aqueous Solutions of Iron(III) Citrate Complexes

et al. 2005

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“…However, there is a vanishingly small concentration of pyrophosphate in the cell and no evidence that sequestered sites with higher concentrations exist (Weaver & Pollock 1989). Other compounds which have been postulated as ligands for iron include amino acids, polypeptides, certain sugars and uncharacterized growth factors (Gessa et al 1983, Tonkovic et al 1983, Bakkeren et al 1985, Wolowiec & Drabent 1985, Andersson & Porath 1986, Inoue et al 1987). The weakness of these studies is that corresponding complexes either have not been isolated from biological sources or have not been identified as part of the mobile, low molecular mass iron pool.…”

Section: R Bohnke and B F Matzankementioning

confidence: 99%

The mobile ferrous iron pool in Escherichia coli is bound to a phosphorylated sugar derivative

Böhnke

Matzanke

1995

Biometals

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Based on in vivo Mössbauer spectroscopy it has previously been demonstrated that the intracellular iron pool of Escherichia coli, grown in iron deficient media supplemented with siderophores as the sole iron source, is dominated by a single Fe2+ and a single Fe3+ species. We have isolated the ferrous ion species and have purified it employing native column PAGE, chromatography and ultrafiltration. The purified compound displays an Mapp of 2.2 kDa and an extremely low isoelectric point (pI) of 1.05. It is shown that this ferrous ion binding compound is neither a protein nor a nucleotide, rather it is composed mainly of phosphorylated sugar derivatives. This compound binds approximately 40% of the cytoplasmic iron. Therefore it is proposed that this oligomeric ferrous carbohydrate phosphate represents the long sought after mobile, low molecular mass iron pool.

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“…Citrate is present in blood plasma in submillimolar concentration and promotes the bioavailability of dietary non-heme iron (it is believed to be one of the major components of a pool of Fe III that is not bound to transferrin) (Parkes et al 1991). Citrate has also been proposed to be a constituent of the low molecular weight cytosolic iron pool (Bakkeren et al 1985). The presence of Fe II and a reducing agent is required for activation of the Krebs cycle enzyme aconitase.…”

Section: Introductionmentioning

confidence: 98%

Iron-citrate complexes and free radicals generation: Is citric acid an innocent additive in foods and drinks?

et al. 2007

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The generation of free radicals (Fenton chemistry) from various iron citrate complexes has been studied. Spin trapping methods have been used. The results can question concerning the innocence of added citric acid in foods and cold drinks. We concluded that in absence of pathological situation citric acid is probably not dangerous but it may become dangerous in situation of oxidative stress and/or iron overload.

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Analysis of iron-binding components in the low molecular weight fraction of rat reticulocyte cytosol

Cited by 66 publications

References 16 publications

High‐Field EPR Study of Frozen Aqueous Solutions of Iron(III) Citrate Complexes

High‐Field EPR Study of Frozen Aqueous Solutions of Iron(III) Citrate Complexes

The mobile ferrous iron pool in Escherichia coli is bound to a phosphorylated sugar derivative

Iron-citrate complexes and free radicals generation: Is citric acid an innocent additive in foods and drinks?

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