Analysis of lateral root growth in Arabidopsis in response to physiologically active auxin analogues

Abstract

The aim of this work was to investigate the formation and development of lateral roots in model trials on Arabidopsis thaliana L. Heynh wild type (Col-0), the alf4-1 mutant and its allele by applying the physiologically active auxin analogues IBA, IAA, TA-12 and TA-14.Differences were observed between the alf4-1 mutant and its allele phenotype in the formation of lateral roots. The application of auxin analogues was unable to restore the formation of lateral roots in the alf4-1 mutant. In some cases, under the impact of IBA (1 µM), a cluster of xylem cells was activated in the pericycle of the primary roots and lateral root primordia were formed. The auxin analogues induced the growth of primary roots in the alf4-1 allele and the formation and growth of lateral roots. The impact of IBA (1 µM), TA-12 (1 mM) and IAA (1 µM) was particularly evident. The intense formation of lateral roots under the impact of IBA and TA-12 could be related with the ability of these compounds to intensify mitotic activity in the apical meristem cells of the lateral roots. New data were obtained, showing that IBA and other physiologically active auxin analogues can modify the root system architecture of the test-plant Arabidopsis.

Root-zone Cooling at High Air Temperatures Enhances Physiological Activities and Internal Structures of Roots in Young Tomato Plants

“…3). In Arabidopsis, root IAA promotes root growth (Novickienė et al, 2010;Ohashi-Ito et al, 2013). Therefore, this result confirms the promotion of root growth during day 0 to 7 of cooling treatment and agrees with the previous study (Nkansah and Ito, 1994).…”

Effect of Root-zone Heating on Root Growth and Activity, Nutrient Uptake, and Fruit Yield of Tomato at Low Air Temperatures

“…In Arabidopsis, IAA promotes root growth (Novickienė et al, 2010;Ohashi-Ito et al, 2013) and xylem development (Deng et al, 2012;Ohashi-Ito et al, 2013). In our study, root-zone heating did not affect the IAA concentration.…”

Lateral root initiation and formation within the parental root meristem of Cucurbita pepo: is auxin a key player?

“…The auxin transport inhibitors used were NPA (0.1-1 µm), 2,3,5-triiodobenzoic acid (TIBA; 1-100 µm) and 1-naphthoxyacetic acid (1-NOA; 25-100 µm). The concentrations of auxin transport inhibitors and auxins used in this study were selected empirically after testing published concentrations (Shen et al, 1988;Kamada et al, 2003;Laňková et al, 2010;Novickienė et al, 2010;Strader et al, 2011) to avoid concentrations that inhibited primary root growth.…”