Analysis of lncRNA-mediated gene regulatory network of Bombyx mori in response to BmNPV infection

Zhang, Shaolun; Yin, Haotong; Shen, Manman; Huang, Haoling; Hou, Qirui; Zhang, Zhendong; Zhao, Weiguo; Guo, Xijie; Wu, Ping

doi:10.1016/j.jip.2020.107323

Cited by 34 publications

(31 citation statements)

References 53 publications

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“…B. mori nucleo-polyhedrovirus (BmNPV) is threatening to economic growth and the stability of sericulture business. The BmNPV infection in silkworms leads to the expression of 4450 lncRNA [ 140 ]. In-silico analysis suggests the target of these differentially expressed lncRNAs to be the genes involved in ubiquitin mediated proteolysis, endocytosis, and lysosomal pathways [ 141 ].…”

Section: Identification and Functional Characterization Of Lncrnasmentioning

confidence: 99%

Long Non-Coding RNAs in Insects

Choudhary

Sharma

Meghwanshi

et al. 2021

Animals

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Only a small subset of all the transcribed RNAs are used as a template for protein translation, whereas RNA molecules that are not translated play a very important role as regulatory non-coding RNAs (ncRNAs). Besides traditionally known RNAs (ribosomal and transfer RNAs), ncRNAs also include small non-coding RNAs (sncRNAs) and long non-coding RNAs (lncRNAs). The lncRNAs, which were initially thought to be junk, have gained a great deal attention because of their regulatory roles in diverse biological processes in animals and plants. Insects are the most abundant and diverse group of animals on this planet. Recent studies have demonstrated the role of lncRNAs in almost all aspects of insect development, reproduction, and genetic plasticity. In this review, we describe the function and molecular mechanisms of the mode of action of different insect lncRNAs discovered up to date.

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Section: Identification and Functional Characterization Of Lncrnasmentioning

confidence: 99%

Long Non-Coding RNAs in Insects

Choudhary

Sharma

Meghwanshi

et al. 2021

Animals

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“…The general high virulence of baculovirus infections has also been exploited for their successful application as biocontrol agents to kill pests. However, although methods such as bulk RNA-seq and proteomics have been used in recent years to try to clarify the interaction between baculovirus and the host (9)(10)(11)(12)(13)(14), major gaps remain regarding the host response to the virus, especially at the level of cellular immunity. Hence, the elucidation of the response of different hemocyte subsets to BmNPV infection is a powerful approach to explore the insect cellular immune response to baculovirus infection.…”

Section: Introductionmentioning

confidence: 99%

Identification of Silkworm Hemocyte Subsets and Analysis of Their Response to Baculovirus Infection Based on Single-Cell RNA Sequencing

et al. 2021

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A wide range of hemocyte types exist in insects but a full definition of the different subclasses is not yet established. The current knowledge of the classification of silkworm hemocytes mainly comes from morphology rather than specific markers, so our understanding of the detailed classification, hemocyte lineage and functions of silkworm hemocytes is very incomplete. Bombyx mori nucleopolyhedrovirus (BmNPV) is a representative member of the baculoviruses and a major pathogen that specifically infects silkworms (Bombyx mori) and causes serious losses in sericulture industry. Here, we performed single-cell RNA sequencing (scRNA-seq) of hemocytes in BmNPV and mock-infected larvae to comprehensively identify silkworm hemocyte subsets and determined specific molecular and cellular characteristics in each hemocyte subset before and after viral infectmadion. A total of 20 cell clusters and their potential marker genes were identified in silkworm hemocytes. All of the hemocyte clusters were infected by BmNPV at 3 days after inoculation. Interestingly, BmNPV infection can cause great changes in the distribution of hemocyte types. The cells appearing in the infection group mainly belong to prohemocytes (PR), while plasmatocytes (PL) and granulocytes (GR) are very much reduced. Furthermore, we found that BmNPV infection suppresses the RNA interference (RNAi) and immune response in the major hemocyte types. In summary, our results revealed the diversity of silkworm hemocytes and provided a rich resource of gene expression profiles for a systems-level understanding of their functions in the uninfected condition and as a response to BmNPV.

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“…Similar to miRNAs in pathogen interaction, lncRNAs are reported to be responsible for a variety of biological processes including immune response. Differentially expressed (DE) lncRNAs could participate in host response to BmNPV infection via interactions with their target genes and miRNAs (S. Zhang et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Expression profile analysis of circular RNAs in BmN cells (Bombyx mori) upon BmNPV infection

Zhang

Shen

Yin

et al. 2020

Arch Insect Biochem Physiol

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The disease caused by Bombyx mori nucleopolyhedrovirus (BmNPV) has always been difficult to control, resulting in tremendous economic losses in the sericulture industry. Although much has been learned about the impact of noncoding RNAs on pathogenesis, the role of circular RNA (circRNA) in insect immunity remains unclear. To explore circRNA regulation involved in BmNPV infection, we used transcriptome analysis of BmN cells with or without BmNPV infection to generate circRNA data set. A total of 444 novel circRNAs were identified in BmN cells, with 198 pervasively distributed both in the control group and BmNPV-infection group. The host genes were enriched inMAPK signaling pathway, dorso-ventral axis formation, and ECM-receptor interaction, which were required for the normal larval growth. A total of 75 circRNAs were differentially expressed (DE) on BmNPV infection. Six downregulated circRNAs were validated by Sanger sequencing and qRT-PCR. DEcircRNA-miRNA-DEmRNA network was constructed based on the six validated cir-cRNAs. Pathway analysis indicated that the predicted target genes were mainly enriched in the metabolic pathway and immune-related signaling pathway. Our results may provide a basis for further studies on circRNA function in BmN cells challenged by BmNPV infection and offer an

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Analysis of lncRNA-mediated gene regulatory network of Bombyx mori in response to BmNPV infection

Cited by 34 publications

References 53 publications

Long Non-Coding RNAs in Insects

Long Non-Coding RNAs in Insects

Identification of Silkworm Hemocyte Subsets and Analysis of Their Response to Baculovirus Infection Based on Single-Cell RNA Sequencing

Expression profile analysis of circular RNAs in BmN cells (Bombyx mori) upon BmNPV infection

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