Analysis of MC-LR and MC-RR in tissue from freshwater fish (Tinca tinca) and crayfish (Procambarus clarkii) in tench ponds (Cáceres, Spain) by liquid chromatography–mass spectrometry (LC–MS)

Ríos, Victoria; Moreno, Isabel; Prieto, Ana I.; Puerto, María; Gutiérrez-Praena, Daniel; Soria-Díaz, M. Eugenia; Cameán, Ana M.

doi:10.1016/j.fct.2013.03.025

Cited by 35 publications

(15 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1). For the fish samples, three common solvent systems were tested, including 80:20 methanol: water (hereafter, 80% methanol) (Smith and Boyer, 2009), 15:4:1 (75:20:5 vol:vol:vol) methanol:water:butanol (Cadel-Six et al, 2014;Ernst et al, 2005;Jia et al, 2014;Xie and Park, 2007b;Zhang et al, 2009), and 5% acetic acid and 0.01 M EDTA (hereafter, AA-EDTA Dai et al, 2008;Guo et al, 2014;Ríos et al, 2013;Schmidt et al, 2013;Wu et al, 2010). For lettuce, two previously used solvent systems, 50:50 methanol:water (hereafter, 50% methanol) (Gutiérrez-Praena et al, 2014) and 80:19:1 methanol:water: trifluoroacetic acid (Li et al, 2014), as well as AA-EDTA were tested.…”

Section: Optimization Of Extraction Conditionsmentioning

confidence: 99%

“…This finding was a bit surprising, given that the previously published studies that used 15:4:1 methanol:water:butanol as a solvent had MC recovery rates that did not exceed 88% (see Table 2). In addition, the single study that used 80% methanol as a solvent reported MC recovery levels that ranged 80% to 99% (Smith and Boyer, 2009), with all studies that used the AA-EDTA solvent system reporting maximum MC recovery rates ranging from 90% to 110% (Dai et al, 2008;Guo et al, 2014;Ríos et al, 2013;Schmidt et al, 2013;Wu et al, 2010).…”

Section: Recovery From Fish Tissuesmentioning

confidence: 99%

See 1 more Smart Citation

Optimization of extraction methods for quantification of microcystin-LR and microcystin-RR in fish, vegetable, and soil matrices using UPLC–MS/MS

et al. 2018

View full text Add to dashboard Cite

Human-driven environmental change has increased the occurrence of harmful cyanobacteria blooms in aquatic ecosystems. Concomitantly, exposure to microcystin (MC), a cyanobacterial toxin that can accumulate in animals, edible plants, and agricultural soils, has become a growing public health concern. For accurate estimation of health risks and timely monitoring, availability of reliable detection methods is imperative. Nonetheless, quantitative analysis of MCs in many types of biological and environmental samples has proven challenging because matrix interferences can hinder sample preparation and extraction procedures, leading to poor MC recovery. Herein, controlled experiments were conducted to enhance the use of ultra-performance liquid-chromatography tandem-mass spectrometry (UPLC-MS/MS) to recover MC-LR and MC-RR at a range of concentrations in seafood (fish), vegetables (lettuce), and environmental (soil) matrices. Although these experiments offer insight into detailed technical aspects of the MC homogenization and extraction process (i.e., sonication duration and centrifugation speed during homogenization; elution solvent to use during the final extraction), they centered on identifying the best (1) solvent system to use during homogenization (2-3 tested per matrix) and (2) single-phase extraction (SPE) column type (3 tested) to use for the final extraction. The best procedure consisted of the following, regardless of sample type: centrifugation speed = 4200 × g; elution volume = 8 mL; elution solvent = 80% methanol; and SPE column type = hydrophilic-lipophilic balance (HLB), with carbon also being satisfactory for fish. For sonication, 2 min, 5 min, and 10 min were optimal for fish, lettuce, and soil matrices, respectively. Using the recommended HLB column, the solvent systems that led to the highest recovery of MCs were methanol:water:butanol for fish, methanol:water for lettuce, and EDTA-NaPO for soils. Given that the recommended procedures resulted in average MC-LR and MC-RR recoveries that ranged 93 to 98%, their adoption for the preparation of samples with complex matrices before UPLC-MS/MS analysis is encouraged.

show abstract

Section: Optimization Of Extraction Conditionsmentioning

confidence: 99%

Section: Recovery From Fish Tissuesmentioning

confidence: 99%

Optimization of extraction methods for quantification of microcystin-LR and microcystin-RR in fish, vegetable, and soil matrices using UPLC–MS/MS

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Mouse bioassay was the first screening method to detect MC-LR [8,9]. The commonly used methods are immunoassays [10], whole cell bioassays [11], protein phosphatase inhibition assays (PPIA) [12][13][14][15], high-performance liquid chromatography (HPLC) [16], and liquid chromatography-mass spectrometry (LC-MS) [17] etc. Mouse bioassay is suitable to get the sample toxicity indication.…”

Section: Introductionmentioning

confidence: 99%

Micro-droplet Hydrodynamic Voltammetry for the Determination of Microcystin-LR Based on Protein Phosphatase

Islam

Sazawa

Sugawara

et al. 2019

J. of Wat. & Envir. Tech.

View full text Add to dashboard Cite

Microcystin-LR (MC-LR) is one of the most frequent and most lethal toxicants and a vital environmental pollutant due to its toxicity and persistence. It is hepatotoxins and have been shown to be potent tumor-promoters which pose a serious threat to human health in the form of chronic exposure through drinking water. In this study, a novel electrochemical method for the determination of MC-LR has been developed using protein phosphatase 2A (PP2A) enzyme inhibition. The rapid and simple determination of PP2A activity was accomplished by means of hydrodynamic voltammetry in a 50 µL micro-droplet with a rotating disk electrode (RDE). The chronoamperometric response were obtained from the electrochemical oxidation of p-aminophenol (PAP) following the enzymatic conversion of p-aminophenyl phosphate (PAPP) as a substrate. PP2A activity estimated by using PAPP substrate is affected by MC-LR, and this phenomenon decreases the current signal of PAP. The IC 50 value was calculated as 0.08 µg/L which is well below the World Health Organization (WHO) provisional guideline value for total MC-LR of 1 µg/L in drinking water. The enzymatic reaction using RDE is possible to perform using fewer chemicals in a rapid manner. Therefore, the method can be very attractive for MC contaminated drinking water routine assessment.

show abstract

“…Numerous analytical methods, including high-performance liquid chromatography (HPLC), 5 liquid chromatography-mass spectrometry (LC-MS), 6 protein phosphatase inhibition assay (PPIA), 7 and whole-cell bioassay, 8 have been used to detect MC-LR. However, these methods all require complicated preparation, expensive heavy equipment, high professional skills, and considerable amount of time, and their selectivity and sensitivity are unsatisfactory.…”

Section: Introductionmentioning

confidence: 99%

Phosphorescent immunosensor for simple and sensitive detection of microcystin-LR in water

Qin

Sun

et al. 2019

RSC Adv.

View full text Add to dashboard Cite

show abstract

Analysis of MC-LR and MC-RR in tissue from freshwater fish (Tinca tinca) and crayfish (Procambarus clarkii) in tench ponds (Cáceres, Spain) by liquid chromatography–mass spectrometry (LC–MS)

Cited by 35 publications

References 39 publications

Optimization of extraction methods for quantification of microcystin-LR and microcystin-RR in fish, vegetable, and soil matrices using UPLC–MS/MS

Optimization of extraction methods for quantification of microcystin-LR and microcystin-RR in fish, vegetable, and soil matrices using UPLC–MS/MS

Micro-droplet Hydrodynamic Voltammetry for the Determination of Microcystin-LR Based on Protein Phosphatase

Phosphorescent immunosensor for simple and sensitive detection of microcystin-LR in water

Contact Info

Product

Resources

About