Analysis of mebendazole polymorphs by Fourier transform IR spectrometry using chemometric methods

Abstract

A diffuse reflectance IR Fourier transform IR spectrometry (DRIFTS) method was developed for the rapid, direct measurement of mebendazole in drugs. Conventional KBr spectra and DRIFTS spectra were compared for the best determination of the active substance in the drug formulations. Two chemometric approaches were used in the data processing: multicomponent partial least squares (PLS2) and principal component regression. The best results were obtained with the PLS2 method.

Simultaneous quantitative analysis of mebendazole polymorphs A–C in powder mixtures by DRIFTS spectroscopy and ANN modeling

“…Form A may occur either due to the followed process of synthesis or due to prolonged thermal exposure of raw pharmaceutical material or commercial product because it is the stable modification at higher temperatures [4,12]. Therefore, several vibrational spectroscopic methods have been proposed for the identification and quantitative analysis of forms C and A in powders [10,13,14], tablets [15,16] or suspensions [10], while the European Pharmacopoeia [3] recommends testing by FTIR spectroscopy. However, none of the proposed methods includes quantification of form B or deals with the simultaneous determination of all three known polymorphs.…”

Polymorphism: The Phenomenon Affecting the Performance of Drugs

“…FT-IR helps to identify the polymorphs by indicating changes in frequencies, relative intensities, band contours and the number of bands. Difference in spectra gives an inference to the internal arrangement of crystals [69][70]. Raman spectroscopy is analogous to FT-IR spectroscopy and is considered an ideal nondestructive tool for polymorphic studies.…”

Quantitative analysis of mebendazole polymorphs in pharmaceutical raw materials using near-infrared spectroscopy

“…It has enough solubility to ensure optimal drug bioavailability and is stable at room temperature up to ±179ºC [1,2,14]. However, all three of these polymorphic forms have been found in pharmaceutical raw material and formulae commercialized worldwide [15][16][17][18].…”

“…These methods are based on a dissolution test [7,19], powder X-ray diffraction (PXRD) [14], ultraviolet spectroscopy [20], Raman spectroscopy [15], middle infrared spectroscopy (MIR) [15][16][17][18]20] and near infrared spectroscopy (NIR) [15]. Among these techniques, X-ray diffraction is considered the most reliable for polymorphism characterization.…”