Analysis of meloxicam by high-performance liquid chromatography with cloud-point extraction

Zhang, Haixia; Choi, Hoo‐Kyun

doi:10.1007/s00216-008-2333-0

Cited by 34 publications

(19 citation statements)

References 18 publications

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“…Chromatographic separations were achieved using a Phenomenex® Luna C18 (250 x 4.6 mm, 5 μm) column. The mobile phase and diluent contained a mixture of methanol:aqueous phosphoric acid (1%, v/v) pH 2.5 (70:30 v/v), 1.0 mL/min flow, PDA detection at 360 nm (Zhang, Choi, 2008;Patel et al, 2011). The injection volume was 20 μL and the column temperature was set at 25 ºC.…”

Section: Chromatographic Conditions and Instrumentsmentioning

confidence: 99%

“…These methods are essential to determine the drug content incorporated into them. Some methods for the quantification of meloxicam in pharmaceutical forms by HPLC method are described in the literature (Zhang, Choi, 2008;Bandarkar, Vavia, 2009;Sahoo et al, 2014). However, there is no specific methodology for nanostructured systems containing meloxicam.…”

Section: Introductionmentioning

confidence: 99%

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Validation of high performance liquid chromatography method for determination of meloxicam loaded PEGylated nanocapsules

Ianiski

Laporta

Rubim

et al. 2015

Braz. J. Pharm. Sci.

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A method to ensure that an analytical method will produce reliable and interpretable information about the sample must first be validated, making sure that the results can be trusted and traced. In this study, we propose to validate an analytical high performance liquid chromatography (HPLC) method for the quantitation of meloxicam loaded PEGylated nanocapsules(M-PEGNC). We performed a validation study, evaluated parameters including specificity, linearity, quantification limit, detection limit, accuracy, precision and robustness. PEGylated nanocapsules were prepared by interfacial deposition of preformed polymer, and the particle size, polydispersity index, zeta potential, pH value and encapsulation efficiency were characterized. The proposed HPLC method provides selective, linear results in the range of 1.0-40.0 μg/mL; quantification and detection limits were 1.78 μg/mL and 0.59 μg/mL, respectively; relative standard deviation for repeatability was 1.35% and intermediate precision was 0.41% and 0.61% for analyst 1 and analyst 2, respectively; accuracy between 99.23 and 101.79%; robustness between 97.13 and 98.45% for the quantification of M-PEGNC. Mean particle diameters were 261 ± 13 nm and 249 ± 20 nm, polydispersity index was 0.15 ± 0.07 and 0.17 ± 0.06, pH values were 5.0 ± 0.2 and 5.2 ± 0.1, and zeta-potential values were -37.9 ± 3.2 mV e -31.8 ± 2.8 mV for M-PEGNC and placebo(B-PEGNC), respectively. In conclusion, the proposed analytical method is suitable for the quality control of M-PEGNC. Moreover, suspensions showed monomodal size distributions and low polydispersity index indicating high homogeneity of formulations with narrow size distributions, and appropriate pH and zeta potential. The extraction process was efficient for release of meloxicam from nanostructured systems.Uniterms: High performance liquid chromatography/quantitative analysis. Meloxicam/determination. PEGylated nanocapsules/quality control. Nanoparticles. Poly(ethylene glycol).Para se assegurar que um método analítico produzirá informação confiável e interpretável sobre a amostra este deve ser inicialmente validado, tornando claro que os resultados podem ser confiados e rastreados. Neste estudo, propomos validar um método de cromatografia líquida de alta eficiência (CLAE) para a quantificação do meloxicam encapsulado em nanocápsulas PEGuiladas (M-PEGNC). Efetuamos a validação, avaliando parâmetros de especificidade, linearidade, limite de quantificação, limite de detecção, exatidão, precisão e robustez. As nanocápsulas PEGuiladas foram preparadas por deposição interfacial do polímero pré-formado e caracterizaram-se o tamanho da partícula, índice de polidispersão, potencial zeta, pH e eficiência de encapsulação. O método de CLAE proposto fornece resultados seletivos e lineares na faixa de 1,0-40,0 mg/mL; limites de quantificação e detecção de 1,78 mg/mL e 0,59 mg/mL, respectivamente; desvio padrão relativo para a repetibilidade de 1,35% e precisão intermediária de 0,41% e 0,61% para o analista 1 e analista 2, respectivamente; exatidã...

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Section: Chromatographic Conditions and Instrumentsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Validation of high performance liquid chromatography method for determination of meloxicam loaded PEGylated nanocapsules

Ianiski

Laporta

Rubim

et al. 2015

Braz. J. Pharm. Sci.

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“…14 It is simple, inexpensive and safe procedure with high efficiency. 15 CPE is compatible with many instruments, therefore it has been successfully applied as an efficient extraction procedure for the separation, preconcentration or purification of a variety of analytes, including organic, [16][17][18] inorganic 19,20 and even pharmaceutical 21 and nano compounds. 22 However, it suffers from some drawbacks that together with fast improvements in micro-extraction techniques made it less attractive.…”

Section: Introductionmentioning

confidence: 99%

Developing a New Micro Cloud Point Extraction Method for Simultaneous Preconcentration and Spectrophotometric Determination of Uranium and Vanadium in Brine

Ghasemi

Kaykhaii

2015

ANAL. SCI.

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A fast, simple, and economical method was developed for simultaneous spectrophotometric determination of uranium(VI) and vanadium(V) in water samples based on micro cloud point extraction (MCPE) at room temperature. This is the first report on the simultaneous extraction and determination of U(VI) and V(V). In this method, Triton X114 was employed as a non-ionic surfactant for the cloud point procedure and 4-(2-pyridylazo) resorcinol (PAR) was used as the chelating agent for both analytes. To reach the cloud point at room temperature, the MCPE procedure was carried out in brine. The factors influencing the extraction efficiency were investigated and optimized. Under the optimized condition, the linear calibration curve was found to be in the concentration range between100 -750 and 50 -600 μg L -1 for U(VI) and V(V), respectively, with a limit of detection of 17.03 μg L -1 (U) and 5.51 μg L -1 (V). Total analysis time including microextraction was less than 5 min.

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“…The reported chromatographic conditions are characterized by isocratic elution, using solvents such as acetonitrile, methanol, formic acid, acetic acid, tetrahydrofuran, phosphate buffer; separation on C8 or C18 columns; UV detection at 355, 360 or 364 nm wavelength. Preparation of plasma samples involves liquid-liquid extraction, solid-phase extraction, cloud point extraction or protein precipitation [1,2,3,[9][10][11][12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Development of a HPLC-UV method for determination of meloxicam in human plasma and pharmaceutical dosage forms

Enikő

Croitoru

Fülöp

et al. 2014

Acta Medica Marisiensis

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Objectives: A simple, quick and low cost HPLC-UV method for assay of meloxicam in plasma and pharmaceutical dosage forms was developed. Methods: Separation and assay of meloxicam, using a simple reverse phase HPLC-UV method was achieved using an Agilent Zorbax SB C18 column, with methanol and 1% aqueous solution of glacial acetic acid as mobile phase. Elution was performed with composition gradient, meloxicam being detected at 355 nm with a 5 minutes analysis time. The method was tested on human plasma and pharmaceutical dosage forms. Results: The retention time of the meloxicam was 3,7 minutes. Regression analysis showed good linearity, with correlation coefficient R= 0,9997; linear regression equation: y = 206,1x -77,5 over the 20-2000 ng/ml concentration range. Limit of detection was determined to be 5 ng/ml and limit of quantification was set at 15 ng/ml. The recovery of the analyte in human plasma was low: 30,50%, however it was reproducible, with a coefficient of variation of 4,83%. The analysis of the tablets resulted in a 85,82% of meloxicam compared to the declared concentration. Conclusions: The method proposed is quick, simple and adequate for detecting the meloxicam in human plasma. Although the recovery rate was low, it was reproducible, which leads to the fact, that improving extraction procedure can optimize the method.

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Analysis of meloxicam by high-performance liquid chromatography with cloud-point extraction

Cited by 34 publications

References 18 publications

Validation of high performance liquid chromatography method for determination of meloxicam loaded PEGylated nanocapsules

Validation of high performance liquid chromatography method for determination of meloxicam loaded PEGylated nanocapsules

Developing a New Micro Cloud Point Extraction Method for Simultaneous Preconcentration and Spectrophotometric Determination of Uranium and Vanadium in Brine

Development of a HPLC-UV method for determination of meloxicam in human plasma and pharmaceutical dosage forms

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