Analysis of Metabolite Compound Profiles of Miana Leaves Endophytic Bacteria (Coleus scutellariodes) using GC-MS

Abstract

Exploration of bioactive compounds from endophytic bacteria as novel source of antibacterial need to be explored, as an alternative to substitute synthetic antibiotics. Based on previous studies, DM6 was a potential isolate that showed antibacterial activity. The isolate has been identified as Brevibacillus sp. based on 16S rRNA analysis. This study aimed to identify antibacterial compounds produced by DM6 isolate using GC-MS. The GC-MS results showed that the chloroform extract of DM6 isolate contained : 1-Octadecene (CAS).alpha.-Octadecene, Hexanedioic acid, dioctyl ester (CAS) Dioctyl adipate, Phenol, 3,5-bis(1,1-dimethylethyl)- (CAS) 3,5-Di-tert-butylphenol, and 1,4-diaza-2,5-dioxobicyclo[4.3.0]nonane. Adipic acid/hexanedioic acid and phenols are also known to contained in the leaves of Coleus scutellariodes. This indicated that endophytic bacteria were able to synthesize antibacterial compounds which similar with the host plant.

Characterization of endophytic bacteria of Mindi (Melia azedarach) and Balsa (Ochroma pyramidale) plants and potential inhibition in plant diseases

“…Pure cultures resulting from biosafety tests were cultured on tryptic soy agar (TSA) media. TSA media is a nutrient-rich medium containing peptones derived from casein, soy meal, agar, and sodium chloride [11]. TSA media can isolate many plant endophytic bacteria [12].…”

Exploring the antibacterial activity of endophytic bacteria from Andaliman (Zanthoxylum acanthopodium DC.) against Bacillus subtilis

“…However, there is no information regarding the antimicrobial activities of endophytic bacteria from Z. acanthopodium. Endophytic bacteria can produce unique natural compounds that are sometimes similar to the compounds produced by their host plants, one of which is the ability to synthesize antibacterial compounds as their host plants (Kusumawati et al 2014). Based on the potential possessed by Z. acanthopodium, it is crucial to study the antimicrobial activity of Z. acanthopodium endophytic bacteria against B. subtilis.…”