Analysis of Metrological Quality and Mechanical Properties of Models Manufactured with Photo-Curing PolyJet Matrix Technology for Medical Applications

Kozior, Tomasz; Bochnia, Jerzy; Gogolewski, Damian; Zmarzły, Paweł; Rudnik, Mateusz; Szot, Wiktor; Szczygieł, Paweł; Musiałek, Mateusz

doi:10.3390/polym14030408

Cited by 18 publications

(9 citation statements)

References 25 publications

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“…Depending on the function that the element performs, various quality criteria are adopted regarding the dimensional and shape accuracy, mechanical properties, etc. The quality of additively manufactured objects is largely dependent on their geometric, dimensional accuracy and surface texture (e.g., surface roughness and waviness), as indicated in [23,24]. From a review of the literature, it was evident that by modifying the printing parameters, for example, reducing the layer thickness, increasing the nozzle diameter, controlling the infill percentage and selecting the right printing orientation and infill pattern, it is possible to considerably improve the tensile strength of printed elements and reduce their porosity.…”

Section: Introductionmentioning

confidence: 99%

The Mechanical Properties of Thin-Walled Specimens Printed from a Bronze-Filled PLA-Based Composite Filament Using Fused Deposition Modelling

2023

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This article focuses on the mechanical property analysis of important models omitted in many scientific papers (thin-walled specimens) printed from innovative material—such as PLA + bronze composite—using fused deposition modelling technology. It discusses the printing process, the measurement of the specimen geometry, the static tensile strength tests and the microscopic examinations conducted with a scanning electron microscope. The findings of this study could be used as an input to further research into the accuracy of filament deposition and the modification of base materials with bronze powder and for the optimization of the machine design, e.g., with the use of cell structures. The experimental results indicated that the thin-walled models fabricated using FDM showed substantial differences in tensile strength, depending on the specimen’s thickness and the printing orientation. It was shown that it was not possible to test thin-walled models located on the building platform along the Z axis due to the lack of sufficient adhesion between the layers.

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Section: Introductionmentioning

confidence: 99%

The Mechanical Properties of Thin-Walled Specimens Printed from a Bronze-Filled PLA-Based Composite Filament Using Fused Deposition Modelling

2023

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“…The loaded tissue-transwell can be cultured in a 6-well dish or in a special 3D-printed housing chamber. Different sizes of this device were designed for mouse/rat, ferret, and human explants and printed using biocompatible plastic Med 610 HQ ( 31 ). After autoclaving the devices, we loaded them with fully brushed WT ferret tracheas and seeded the lumen with 2*10 6 Tomato+ ferret airway basal cells.…”

Section: Resultsmentioning

confidence: 99%

Development and characterization of ferret ex vivo tracheal injury and cell engraftment model

et al. 2023

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The field of airway biology research relies primarily on in vitro and in vivo models of disease and injury. The use of ex vivo models to study airway injury and cell-based therapies remains largely unexplored although such models have the potential to overcome certain limitations of working with live animals and may more closely replicate in vivo processes than in vitro models can. Here, we characterized a ferret ex vivo tracheal injury and cell engraftment model. We describe a protocol for whole-mount staining of cleared tracheal explants, and showed that it provides a more comprehensive structural overview of the surface airway epithelium (SAE) and submucosal glands (SMGs) than 2D sections, revealing previously underappreciated structural anatomy of tracheal innervation and vascularization. Using an ex vivo model of tracheal injury, we evaluated the injury responses in the SAE and SMGs that turned out to be consistent with published in vivo work. We used this model to assess factors that influence engraftment of transgenic cells, providing a system for optimizing cell-based therapies. Finally, we developed a novel 3D-printed reusable culture chamber that enables live imaging of tracheal explants and differentiation of engrafted cells at an air-liquid interface. These approaches promise to be useful for modeling pulmonary diseases and testing therapies.Graphical abstract1,2. We describe here a method for differential mechanical injury of ferret tracheal explants that can be used to evaluate airway injury responses ex vivo. 3. Injured explants can be cultured at ALI (using the novel tissue-transwell device on the right) and submerged long-term to evaluate tissue-autonomous regeneration responses. 4. Tracheal explants can also be used for low throughput screens of compounds to improve cell engraftment efficiency or can be seeded with particular cells to model a disease phenotype. 5. Lastly, we demonstrate that ex vivo-cultured tracheal explants can be evaluated by various molecular assays and by immunofluorescent imaging that can be performed live using our custom-designed tissue-transwell.

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“…Further, 3D printing can be categorized into inkjet, laser-assisted, and extrusion-based bioprinting [ 4 ]. Nowadays, extrusion-based bioprinter is one of the most popular techniques in biomedical applications because of its ability to print cells, and various biomaterials with a wide range of viscosity [ 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

Printability and Cell Viability in Extrusion-Based Bioprinting from Experimental, Computational, and Machine Learning Views

Malekpour

Chen

2022

JFB

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Extrusion bioprinting is an emerging technology to apply biomaterials precisely with living cells (referred to as bioink) layer by layer to create three-dimensional (3D) functional constructs for tissue engineering. Printability and cell viability are two critical issues in the extrusion bioprinting process; printability refers to the capacity to form and maintain reproducible 3D structure and cell viability characterizes the amount or percentage of survival cells during printing. Research reveals that both printability and cell viability can be affected by various parameters associated with the construct design, bioinks, and bioprinting process. This paper briefly reviews the literature with the aim to identify the affecting parameters and highlight the methods or strategies for rigorously determining or optimizing them for improved printability and cell viability. This paper presents the review and discussion mainly from experimental, computational, and machine learning (ML) views, given their promising in this field. It is envisioned that ML will be a powerful tool to advance bioprinting for tissue engineering.

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Analysis of Metrological Quality and Mechanical Properties of Models Manufactured with Photo-Curing PolyJet Matrix Technology for Medical Applications

Cited by 18 publications

References 25 publications

The Mechanical Properties of Thin-Walled Specimens Printed from a Bronze-Filled PLA-Based Composite Filament Using Fused Deposition Modelling

The Mechanical Properties of Thin-Walled Specimens Printed from a Bronze-Filled PLA-Based Composite Filament Using Fused Deposition Modelling

Development and characterization of ferret ex vivo tracheal injury and cell engraftment model

Printability and Cell Viability in Extrusion-Based Bioprinting from Experimental, Computational, and Machine Learning Views

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