Analysis of MicroRNA Expression Profile Identifies Novel Biomarkers for Non-small Cell Lung Cancer

Xu, Chi; Zheng, Yisheng; Lian, Duohuang; Ye, Shixin; Yang, Jing; Zeng, Zhiyong

doi:10.5301/tj.5000224

Cited by 35 publications

(36 citation statements)

References 55 publications

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“…De-regulation of miRNA, such as miR-21, miR-22, miR-448, miR-654-3p, miR-205, miR-216a, miR-143, and miR-181a in NSCLC is a key factor underlying tumorigenesis (Wang et al, 2014;Xu et al, 2015). Human miR-204 is down-regulated in several tumor types, including NSCLC Shi et al, 2014;Xia et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

“…miRNA are important regulators that are significantly involved in the development of human diseases. Recent studies have shown a close correlation between dysregulated miRNA and carcinogenesis and cancer progression (Del Vescovo et al, 2014;Xu et al, 2015). For example,, and miR-125a-5p expression is associated with the progression and prognosis of non-small cell lung cancer (Kim et al, 2014;Zhu et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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miR-204 suppresses non-small-cell lung carcinoma (NSCLC) invasion and migration by targeting JAK2

Wang

Lv²,

Zhou

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Aberrant expression of microRNA is associated with the development and progression of cancers. down-regulation has been previously demonstrated in non-small-cell lung carcinoma (NSCLC); however, the underlying mechanism by which miR-204 suppresses tumorigenesis in NSCLC remains elusive. In this study, miR-204 expression was found to be down-regulated, and that of Janus kinase 2 (JAK2) was found to be up-regulated in four NSCLC cell lines (A549, H1299, H1650, and H358) compared to the normal lung cell line. The overexpression of miR-204 suppressed the invasive and migratory capacities of H1299 cells. A luciferase assay confirmed that the binding of miR-124 to the -untranslated region of JAK2 inhibited the expression of JAK2 proteins in H1299 cells. JAK-2 overexpression effectively reversed miR-204-repressed NSCLC metastasis. Taken together, our findings revealed that miR-204 functions as a tumor suppressor in NSCLC by targeting JAK2, and that miR-204 may therefore serve as a biomarker for the diagnosis and treatment of NSCLC.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

miR-204 suppresses non-small-cell lung carcinoma (NSCLC) invasion and migration by targeting JAK2

Wang

Lv²,

Zhou

et al. 2016

Genet. Mol. Res.

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“…It is believed that the dysregulation of miRNAs is closely related to the development and progression of a number of cancers 4, 5, 12, 13, 21. The down‐regulation of miR‐138 in NSCLC has been consistently observed by multiple laboratories 16, 19.…”

Section: Discussionmentioning

confidence: 99%

MiR‐138 inhibits cell proliferation and reverses epithelial‐mesenchymal transition in non‐small cell lung cancer cells by targeting GIT1 and SEMA4C

Wang

Wen

et al. 2015

J Cellular Molecular Medi

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Non‐small‐cell lung cancer (NSCLC) is one of the most common and lethal malignant tumours worldwide with a poor 5‐year survival rate. Recent studies indicated that miRNAs have been involved in the tumorigenic driver pathways in NSCLC, but the relevant molecular mechanisms are not well‐understood. In this study, we investigated the biological functions and molecular mechanisms of miR‐138 in human NSCLC. The effects of miR‐138 on the NSCLC cell growth and epithelial‐mesenchymal transition (EMT) were first examined. Then the targeting connections of miR‐138 with G‐protein‐coupled receptor kinase‐interacting protein 1 (GIT1) and semaphorin 4C (SEMA4C) were confirmed by dual luciferase reporter assays. Finally, the effects of GIT1 and SEMA4C on the NSCLC cell growth and EMT were investigated respectively. We found that the ectopic expression of miR‐138 resulted in a significant inhibition of NSCLC growth and reversion of EMT. GIT1 and SEMA4C were identified as two novel targets of miR‐138. Furthermore, GIT1 and SEMA4C knockdown inhibited the cell growth and reversed EMT, just like the effects of miR‐138 overexpression on NSCLC cells, whereas ectopic expression of GIT1 and SEMA4C partly rescued the suppressive effects of miR‐138 in NSCLC cells. These data represent a crucial step towards the understanding of the novel roles and molecular mechanism of miR‐138, GIT1 and SEMA4C in NSCLC progression, which may provide some new targets or prognostic biomarkers for NSCLC treatment, thus having implications in translational oncology.

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“…The online literature database PubMed was used to identify lung cancer miRNA expression profiling studies published from January 2003 until May 2014 (last accessed on 15 May 2014), by means of the 'Medical Subject Heading' terms: 'NSCLC' and 'microRNAs' in combination with the keywords 'profiling' and 'humans'. The results of previous studies to identify circulating miRNAs specific to lung cancer were inconsistent (24)(25)(26)(27), and potential biomarkers remain to be elucidated and exploited. Based on these previous findings, a list of 15 miRNAs was compiled, whose expression has already been reported as deregulated in cancer, or has not been investigated (Table III).…”

Section: Methodsmentioning

confidence: 99%

Role of deregulated microRNAs in non-small cell lung cancer progression using fresh-frozen and formalin-fixed, paraffin-embedded samples

et al. 2015

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Abstract. Non-small cell lung cancer (NSCLC) is responsible for the highest number of cancer-associated mortalities worldwide, and the five-year survival rate is <15% following the initial diagnosis. MicroRNAs (miRNAs) serve important functions in a number of human diseases, including cancer. The present study investigated the expression status, clinical relevance and functional role of miRNA in NSCLC. miRNA expression profiling was performed in lung adenocarcinoma and adjacent unaffected lung tissues using 47 groups of fresh-frozen (FF) and 45 of formalin-fixed, paraffin-embedded (FFPE) samples from 11 pulmonary bulla. miR-21, -30e, -363 and -623 were further examined for differential expression in two independent cohorts. Other miRNAs, including miR-5100 and miR-650, were upregulated, while miR-10a and -26b were downregulated in FF NSCLC tissues. The associations between these miRNAs and their clinicopathological features were also investigated. miR-363, -10a and -145 were associated with lymph node status (P= 0.002, 0.005 and 0.007, respectively) and miR-650 and -145 were associated with differentiation (P=0.01 and 0.05, respectively). No associations were identified for the other miRNAs examined. In the FFPE NSCLC samples, miR-30e-5p correlated with the differentiation of the tissue (P= 0.011). The present study indicates that these miRNAs may be appropriate candidates for molecular diagnostic and prognostic markers in NSCLC.

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Analysis of MicroRNA Expression Profile Identifies Novel Biomarkers for Non-small Cell Lung Cancer

Abstract: Our study may provide the groundwork for further clinical molecular target therapy experiments in NSCLC.BAC

Cited by 35 publications

References 55 publications

miR-204 suppresses non-small-cell lung carcinoma (NSCLC) invasion and migration by targeting JAK2

miR-204 suppresses non-small-cell lung carcinoma (NSCLC) invasion and migration by targeting JAK2

MiR‐138 inhibits cell proliferation and reverses epithelial‐mesenchymal transition in non‐small cell lung cancer cells by targeting GIT1 and SEMA4C

Role of deregulated microRNAs in non-small cell lung cancer progression using fresh-frozen and formalin-fixed, paraffin-embedded samples

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