Role of deregulated microRNAs in non-small cell lung cancer progression using fresh-frozen and formalin-fixed, paraffin-embedded samples

Abstract

Abstract. Non-small cell lung cancer (NSCLC) is responsible for the highest number of cancer-associated mortalities worldwide, and the five-year survival rate is <15% following the initial diagnosis. MicroRNAs (miRNAs) serve important functions in a number of human diseases, including cancer. The present study investigated the expression status, clinical relevance and functional role of miRNA in NSCLC. miRNA expression profiling was performed in lung adenocarcinoma and adjacent unaffected lung tissues using 47 groups of fresh-frozen (FF) and 45 of formalin-fixed, paraffin-embedded (FFPE) samples from 11 pulmonary bulla. miR-21, -30e, -363 and -623 were further examined for differential expression in two independent cohorts. Other miRNAs, including miR-5100 and miR-650, were upregulated, while miR-10a and -26b were downregulated in FF NSCLC tissues. The associations between these miRNAs and their clinicopathological features were also investigated. miR-363, -10a and -145 were associated with lymph node status (P= 0.002, 0.005 and 0.007, respectively) and miR-650 and -145 were associated with differentiation (P=0.01 and 0.05, respectively). No associations were identified for the other miRNAs examined. In the FFPE NSCLC samples, miR-30e-5p correlated with the differentiation of the tissue (P= 0.011). The present study indicates that these miRNAs may be appropriate candidates for molecular diagnostic and prognostic markers in NSCLC.

miR-363-3p inhibits tumor growth by targeting PCNA in lung adenocarcinoma

“…In our previous study, we identified miR-363-3p was a potential tumor suppressor in lung cancer [22]. A previous study of liver cancer indicated that miR-363-3p can reduced tumorigenesis by inhibiting the G1 to S phase transition [11].…”

Correlation between microRNA expression, clinicopathological characteristics, and prognosis in patients with Non‐small cell Lung Cancer: A retrospective study

“…MiRNA expression was represented by 2−ΔCT, where ΔCT = (CT MiRNA of case or control minus CT reference sample). SnRNA U6 was used as an endogenous control to normalize all samples …”

Molecular Nodal Staging Using miRNA Expression in Lung Cancer Patients by Endobronchial Ultrasound-Guided Transbronchial Needle Aspiration

“…Previous reports have shown that miRNAs participate in some cell signaling processes, including apoptosis, cell proliferation, and epithelial-to-mesenchymal transition [19, 20]. Quantification of miRNA expression can be performed even using relatively degraded samples, such as FFPE samples, in a PCR-based test (real-time PCR) [14, 21-23]. In this study, we used FFPE samples as well as snap-frozen samples (different quality of RNA) and examined the expression of several candidate miRNAs.…”