Analysis of microscopic bone properties in an osteoporotic sheep model: a combined biomechanics, FE and ToF-SIMS study

Müller, R; Henß, Anja; Kampschulte, Marian; Rohnke, Marcus; Langheinrich, Alexander C.; Heiß, Christian; Janek, Jürgen; Voigt, Axel; Wilke, Hans–Joachim; Ignatius, Anita; J, Herfurth; Khassawna, Thaqif El; Deutsch, Andreas

doi:10.1098/rsif.2018.0793

Cited by 11 publications

(10 citation statements)

References 53 publications

(87 reference statements)

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“…All animals had at least one-week acclimatization period prior to the OVX. General anesthesia was prevalently induced by intravenous injection of ketamine and propofol and prolonged with isoflurane in oxygen through an endotracheal tube [ 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 ]. Antibiotic prophylaxis was provided by intravenous injection at the time of anesthesia induction.…”

Section: Resultsmentioning

confidence: 99%

“…The success of OVX in sheep was mostly confirmed by iliac crest and/or lumbar vertebra microcomputed tomography (CT) analyses [ 23 , 25 , 26 , 27 , 31 , 32 , 39 , 40 ] as well as by quantitative computed tomography (qCT), which measured the BMD using a X-ray CT scanner with a calibration standard to convert Hounsfield units (HU) of the CT image to bone mineral density values [ 24 , 28 , 29 ]. BMD was also measured by dual-energy X-ray absorptiometry (DXA) in iliac crest, femora, and lumbar spine [ 25 , 34 , 35 , 36 , 37 , 40 ]. Same studies also evaluated OVX through analysis of biochemical markers of bone, such as osteocalcin, C-terminal cross-linked telopeptides of type I collagen (CTXI), alkaline phosphatase (b-ALP), and by changes in hormonal profile (circulating concentrations of estradiol) [ 27 , 28 , 36 , 38 , 39 ].…”

Section: Resultsmentioning

confidence: 99%

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Osteoporosis Preclinical Research: A Systematic Review on Comparative Studies Using Ovariectomized Sheep

Salamanna

Contartese

Veronesi

et al. 2022

IJMS

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Sheep ovariectomy (OVX) alone or associated to steroid therapy, deficient diet, or hypothalamic–pituitary disconnection has proven to be of critical importance for osteoporosis research in orthopedics. However, the impact of specific variables, such as breed, age, diet, time after OVX, and other variables, should be monitored. Thus, the design of comparative studies is mandatory to minimize the impact of these variables or to recognize the presence of unwanted variables as well as to better characterize bone remodeling in this model. Herein, we conducted a systematic review of the last 10 years on PubMed, Scopus, and Web of Knowledge considering only studies on OVX sheep where a control group was present. Of the 123 records screened, 18 studies were included and analyzed. Results showed that (i) Merino sheep are the most exploited breed; (ii) 5–6 years of age is the most used time for inducing OVX; (iii) ventral midline laparotomy is the most common approach to induce OVX; (iv) OVX associated to steroid therapy is the most widely used osteoporosis model; and (v) success of OVX was mostly verified 12 months after surgery. In detail, starting from 12 months after OVX a significant decline in bone mineral density and in microarchitectural bone parameters as well as in biochemical markers were detected in all studies in comparison to control groups. Bone alteration was also site-specific on a pattern as follows: lumbar vertebra, femoral neck, and ribs. Before 12 months from OVX and starting from 3–5 months, microarchitectural bone changes and biochemical marker alterations were present when osteoporosis was induced by OVX associated to steroid therapy. In conclusion, OVX in sheep influence bone metabolism causing pronounced systemic bone loss and structural deterioration comparable to the situation found in osteoporosis patients. Data for treating osteoporosis patients are based not only on good planning and study design but also on a correct animal use that, as suggested by 3Rs principles and by ARRIVE guidelines, includes the use of control groups to be directly contrasted with the experimental group.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Osteoporosis Preclinical Research: A Systematic Review on Comparative Studies Using Ovariectomized Sheep

Salamanna

Contartese

Veronesi

et al. 2022

IJMS

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“…The scans were segmented by a global thresholding procedure (trabecular attenuation = 130; cortical attenuation = 200 in permille of the total gray value range). Morphometric parameters were determined with a direct 3D approach ( 8 ) in three different pre-selected analysis regions by means of customized software developed on the proprietary Image Processing Language v5.15 (Scanco Medical). We measured humerus length and bone volume fraction (BV/TV, %) ( 9 ) along the entire bone.…”

Section: Methodsmentioning

confidence: 99%

Different Effects of Soy and Whey on Linear Bone Growth and Growth Pattern in Young Male Sprague-Dawley Rats

et al. 2021

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The aim of this investigation was to determine the better protein for supporting optimal linear growth, as the exact composition and benefits of specific dietary proteins in supporting linear growth is unknown. In the current study, we compared the effect of soy and whey proteins, both proteins contain all essential amino acids and are considered the best proteins in their categories. Young male rats were subjected to multiple feeding protocols using iso-energetic diets containing soy or whey as the sole protein source. The rats were allowed to eat ad libitum for 11, 24, or 74 days in the first set of experiments, and the soy group was pair-fed to the whey group in the second set. The differences in weight gain, food consumption, and humeri length of the soy group that were greater at the beginning of the ad libitum experiments lessened over time. Pair-fed experiments revealed that the increased weight and humeri length resulted from the differences in food consumption. However, other parameters were protein specific. Bone quality, which was better in the soy group at 24 days, was matched by the whey group and even surpassed that of the soy group in the long-term experiment, with a significantly greater bone mineral density, cortical thickness, and growth plate. Although in the short term the levels of insulin like growth factor (IGF)-I were similar between the groups, IGF-I increased with age in the whey group, and the levels at the long-term experiment were significantly higher compared to the soy group. Furthermore, using the pair fed setup made it clear that when the difference in food consumption were no longer playing part, whey was more efficient in increasing IGF-I. There were no indications of metabolic sequelae. Although the use of soy is gaining in popularity as a sustainable protein, our findings indicate a better effect of whey on linear growth by leading to slower growth with better-organized epiphyseal growth plates and bone quality.

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“…Parallel to the work conducted on modelling human bone, there have been efforts to model bone of several animals. Studies range from small animals such as mice [287,[329][330][331][332][333][334][335][336], rats [288,[337][338][339][340][341][342][343] and zebrafish [344], to medium-sized animals such as dogs [345], as well as large animals such as pigs [346][347][348][349][350][351][352], sheep [353][354][355], bovine [220,[356][357][358][359][360][361][362][363] and horses [273,364].…”

Section: Remark 27 (Animal Bone Modelling and Simulation)mentioning

confidence: 99%

“…12A 1 : Usage of animal bone experimental data to estimate mechanical properties [220,287,288,330,331,340,342,347,363,369] and conception of animal bone failure models [331,338,349,351,356,358,362] that may be similar to human bone failure models [342,344,370]. This directive includes studies in which pathological changes to the skeleton are purposefully induced in test animals by genetic manipulation [371] or malnutrition [332,338,354] in order to create models to study osteoporosis [338,354].…”

Section: Remark 27 (Animal Bone Modelling and Simulation)mentioning

confidence: 99%

Patient-Specific Bone Multiscale Modelling, Fracture Simulation and Risk Analysis—A Survey

et al. 2019

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This paper provides a starting point for researchers and practitioners from biology, medicine, physics and engineering who can benefit from an up-to-date literature survey on patient-specific bone fracture modelling, simulation and risk analysis. This survey hints at a framework for devising realistic patient-specific bone fracture simulations. This paper has 18 sections: Section 1 presents the main interested parties; Section 2 explains the organzation of the text; Section 3 motivates further work on patient-specific bone fracture simulation; Section 4 motivates this survey; Section 5 concerns the collection of bibliographical references; Section 6 motivates the physico-mathematical approach to bone fracture; Section 7 presents the modelling of bone as a continuum; Section 8 categorizes the surveyed literature into a continuum mechanics framework; Section 9 concerns the computational modelling of bone geometry; Section 10 concerns the estimation of bone mechanical properties; Section 11 concerns the selection of boundary conditions representative of bone trauma; Section 12 concerns bone fracture simulation; Section 13 presents the multiscale structure of bone; Section 14 concerns the multiscale mathematical modelling of bone; Section 15 concerns the experimental validation of bone fracture simulations; Section 16 concerns bone fracture risk assessment. Lastly, glossaries for symbols, acronyms, and physico-mathematical terms are provided.

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Analysis of microscopic bone properties in an osteoporotic sheep model: a combined biomechanics, FE and ToF-SIMS study

Cited by 11 publications

References 53 publications

Osteoporosis Preclinical Research: A Systematic Review on Comparative Studies Using Ovariectomized Sheep

Osteoporosis Preclinical Research: A Systematic Review on Comparative Studies Using Ovariectomized Sheep

Different Effects of Soy and Whey on Linear Bone Growth and Growth Pattern in Young Male Sprague-Dawley Rats

Patient-Specific Bone Multiscale Modelling, Fracture Simulation and Risk Analysis—A Survey

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