Analysis of mitochondrial DNA HVR1 haplotype of pure-bred domestic dogs in Japan

Sugiyama, Sho; Chong, Yong Hwa; Shito, Masayuki; Kasuga, Manami; Kawakami, Tsuyoshi; Udagawa, Chihiro; Aoki, Hiroshi; Bonkobara, Makoto; Tsuchida, Shuichi; Sakamoto, Atsushi; Okuda, Hiroshi; Nagai, Atsushi; Omi, Toshinori

doi:10.1016/j.legalmed.2013.08.005

Cited by 8 publications

(5 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…So, the high diversities reported in the current study probably explain the lack of differentiation between the Egyptian Baladi and German shepherd dogs in Egypt (23). These results agreed with the previous studied (11,24) which reported high haplotype diversities although using different dog breeds and nucleotide numbers. Moreover, the Egyptian Baladi dogs had high haplotype diversity almost, as high as that noticed in the case of German shepherd dogs.…”

Section: Discussionsupporting

confidence: 92%

“…The mtDNA is highly informative because of its characteristics such as small molecular weight, high variability and maternal transmission (8)(9)(10). The canine mtDNA is approximately 16,728 bp, which consists of 13 proteincoding genes, 22 tRNAs, 2 rRNAs, and polymorphic non-coding regions referred to as hypervariable regions (HVR1 and HVR2) (11). HVR1 is highly polymorphic and can be effectively used in forensic investigations because it can be successfully amplified from limited or severely degraded DNA (7,12).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Genetic Diversity Among Two Common Populations of Canis Lupus Familiaris in Egypt by Using Mitochondrial Dna Hvr1 Sequence

Elmadawy,

El-Kassas,

Abdo

et al. 2019

SVR

View full text Add to dashboard Cite

The current study aimed to investigate the variation of mtDNA hypervariable region 1 (HVR1) among Egyptian Baladi and German shepherd dogs in Egypt with respect to their phylogenetic origin. Blood samples were obtained from two dog breeds; Egyptian Baladi (n = 46) and German shepherd (n = 42) and used for genomic DNA extraction and PCR amplification of the mtDNA HVR1 using primers H15360 and L16106. The determined haplotypes were aligned to the sequences of the first published dog mitochondrial genome (Accession No. U96639). We identified 22 different haplotypes from 34 single nucleotide polymorphisms (SNPs) including 2 insertion-deletion polymorphisms among the Egyptian Baladi dogs and 12 haplotypes from 22 SNPs among the German shepherd dogs. Four haplogroups (A, B, C, and D) were identified in the two breeds, their distribution includes 78% of Egyptian Baladi dogs and 76% of German shepherd dogs, respectively were located in the haplogroup A. While 19 % of the German shepherd and 15% of Egyptian Baladi dogs were found in the haplogroup B. 5% of the detected haplotypes of the two breeds were belonged to haplogroup C. 2% of the detected haplotypes of Egyptian Baladi dogs were classified to a haplogroup D. High haplotype and nucleotide diversities were found in the two breeds indicating a lack of genetic differentiation and a recent population growth. The later was confirmed in the Egyptian dogs with the negative values of the neutrality tests and their clustering in the same clade within the phylogenetic tree.

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Genetic Diversity Among Two Common Populations of Canis Lupus Familiaris in Egypt by Using Mitochondrial Dna Hvr1 Sequence

Elmadawy,

El-Kassas,

Abdo

et al. 2019

SVR

View full text Add to dashboard Cite

show abstract

“…The utility of mitochondrial DNA (mtDNA) [5][6][7][8][9][10][11][12][13][14][15][16] and/or short tandem repeat (STR) polymorphisms in forensic analysis has been extended in recent years [17][18][19][20][21][22][23][24][25]. In Japan, very little research on forensic genetics has focused on STR typing in dogs.…”

Section: Introductionmentioning

confidence: 99%

Allele Frequencies and the Probability of Identity for 18 STR Loci Generated via the Canine GenotypesTM Panel 2.1 Kit in Golden Retriever, Miniature Dachshund, and Shiba Inu Groups for Forensic Applications

Kunita,

Udagawa,

Inagaki

et al. 2023

Preprint

View full text Add to dashboard Cite

Similarly to Europe and the United States, the need for the forensic DNA identification of dogs is increasing in Japan. Because few studies use commercial genotyping kits, we examine the effectiveness of individual DNA identification using the Canine Genotypes Panel 2.1 Kit on limited samples of dogs bred in Japan. We used Genomic DNA extracted from blood in non-related 50 Golden Retrievers, 50 Miniature Dachshunds, and 50 Shiba Inus bred in Japan. We investigated 18 canine STR markers (PEZ02, PEZ17, FH2017, FH2309, PEZ05, FH2001, FH2328, FH2004, FH2361, PEZ21, FH2054, FH3377, FH2107, FH2088, vWF.X, FH2010, PEZ16, and FH3313) and one sex-related marker (ZFX.Y) according to the manufacturer’s instructions. Allele frequency, He, Ho, p-value, PD, PE, PIC, and MP were calculated for each marker. Random Match probability based on 18 STR loci was subsequently estimated to be 3.257 × 10−16 in Golden Retrievers, 3.933 × 10−18 in Miniature Dachshunds, and 2.107 × 10−18 in the Shiba Inus breed. There are a few studies that have used this kit in Japan. The results suggest that the kit with 18 autosomal STR loci and one sex marker is effective in forensic applications.

show abstract

“…These analyzes include i.a. : polymorphism analysis of the mitochondrial control region of DNA (Okumura et al 1996, 1998, 1999, Webb et al 2009, Sindičić et al 2011, Sugiyama et al 2013, microsatellite length polymorphism analyzes (Koskinen and Bred-Genetic diversity of native Japanese dog breeds in the primitive type MARTA KLOCH, ANDRZEJ ŻYCZYŃSKI, ZUZANNA NOWAK--ŻYCZYŃSKA Faculty of Animal Sciences, Warsaw University of Life Sciences -SGGW backa 2000, Kim et al 2001, Irion et al 2003, Schelling et al 2004, Cho 2005, Ye et al 2009, Phavaphutanon and Laop 2011, and analysis of coding sequences (Niimi et al 2001).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of native Japanese dog breeds in the primitive type

Kloch

Nowak-Życzyńska

Zyczynski

2019

Annals of Warsaw University of Life Sciences - SGGW - Animal Science

View full text Add to dashboard Cite

show abstract

Analysis of mitochondrial DNA HVR1 haplotype of pure-bred domestic dogs in Japan

Cited by 8 publications

References 19 publications

Genetic Diversity Among Two Common Populations of Canis Lupus Familiaris in Egypt by Using Mitochondrial Dna Hvr1 Sequence

Genetic Diversity Among Two Common Populations of Canis Lupus Familiaris in Egypt by Using Mitochondrial Dna Hvr1 Sequence

Allele Frequencies and the Probability of Identity for 18 STR Loci Generated via the Canine GenotypesTM Panel 2.1 Kit in Golden Retriever, Miniature Dachshund, and Shiba Inu Groups for Forensic Applications

Genetic diversity of native Japanese dog breeds in the primitive type

Contact Info

Product

Resources

About