Analysis of monosaccharides in the saffron corm glycoconjugate by capillary electrophoresis

Abstract

The monosaccharides in the saffron corm glycoconjugate were separated by capillary electrophoresis (CE) coupled with pre-column derivatization. 4-Methoxyaniline was used as derivatization reagent. The derivatization and CE separation conditions were investigated. The ultraviolet detection wavelength was 234 nm. The maximum yield of this derivatization reaction was obtained under the presence of 9.5% (v/v) acetic acid at 80 degrees C for 2 h. An uncoated fused-silica capillary of 50 microm i.d. and 50/60 cm length (effective length/total length) was employed, and a pressure injection (3.4475 kPa, 5 s) was applied. The baseline separation of 11 monosaccharides and disaccharides (lyxose, xylose, ribose, glucose, mannose, galactose, rhamnose, cellobiose, maltose, lactose, fructose) was reached at 25 degrees C, 20 kV of separation voltage and with 350 mmol/L boric acid (pH 10.21) as running buffer. The developed method has been successfully applied to quantitatively determine the components of saffron corm glycoconjugate, and the results showed that the recovery of each monosaccharide was in the range of 94.3% - 105.4%, the relative standard deviation was 3.3% - 4.6%.

Determination and analysis of monosaccharides in Polygonatum cyrtonema Hua polysaccharides from different areas by ultra‐high‐performance liquid chromatography quadrupole trap tandem mass spectrometry

“…In recent years, the qualitative and quantitative determination methods of monosaccharides mainly include HPCE, GC, HPLC, and UHPLC with quadrupole trap‐MS/MS (QTRAP‐MS/MS) [ 18 , 19 , 20 , 21 ]. The low sensitivity and poor reproducibility of the HPCE greatly limit its application [ 22 ]. The operation of the GC method is tedious because of the many types of reagents and their long duration [ 23 ].…”

HPLC Method for Microanalysis and Pharmacokinetics of Marine Sulfated Polysaccharides, Propylene Glycol Alginate Sodium Sulfate

“…Usually monosaccharide derivatization with ultraviolet or fluorophotomeric groups is required for separation and detection by HPLC (Bao et al 2002;Xing et al 2011). Currently, monosaccharide derivatization reagents include PMP (Honda et al 1989), p-aminobenzoic acid (Hao et al 2007), p-anisidine (Ma et al 2012), 2-aminopyridine (Tomiya et al 1987), and so on. The majority of derivatization is based on the reductive amination reaction.…”