Analysis of MYO-Inositol effect on spermatozoa motility, in hyper viscous ejaculates and in patients with grades II and III varicocele

Abstract

The goal of this study is to evaluate MYOInositol effects on spermatozoa motility, in patients’ ejaculates with severe varicocele or hyper viscosity. The study included normal viscosity ejaculate from 30 patients affected by varicocele and hyper viscosity ejaculate from 33 patients without any testicular pathologies. All selected samples showed sperm concentration > 2 million/ml and progressive motility < 32%. In both groups, the pellet obtained after centrifugation in buffered medium, was divided in two aliquots, both incubated for 15 minutes at 37°C: one with MYO-Inositol and the other one, as control, only in phosphate buffered saline (PBS). Afterwards, the sperm progressive motility was assessed using Computer Assisted Sperm Analysis (CASA system). Incubation with MYO-Inositol improved sperm progressive motility in high viscosity samples compared to control group (38.9% ± 3.0 vs 24.35% ± 2.41, respectively; p ≤ 0.0001). Conversely, no statistically significant difference was observed in total sperm progressive motility in varicocele samples compared with control group (22.7% ± 2.07 vs 26.7% ± 3.31, respectively; p = 0.085). The MYO-Inositol positive effect on spermatozoa motility may depend on the type of sperm damage: heavy structural and biochemical defects which typically affects patients with varicocele are not restored by Inositol. On the contrary, MYOInositol is able to improve sperm motility in semen samples with high viscosity, since those samples show no substantial structural sperm defects.

Respiratory Mitochondrial Efficiency and DNA Oxidation in Human Sperm after In Vitro Myo-Inositol Treatment

“…The treated sperm did not show appreciable ultrastructural changes; however, it may be highlighted that treated sperm appear extremely “clean” from any amorphous fibrous material ( Figure 3 C–D) which instead was clearly evident on the surface and in the surrounding environment of the untreated sperm ( Figure 3 A–B). This could depend on the ability of myo-inositol to disrupt the mucoid masses of the seminal fluid, in agreement with what was claimed by Scarselli et al [ 39 ]. Moreover, in treated samples, several sperm with uncurled tails with well-organized mitochondrial sheath can be frequently found in the same field of observation, that is an unusual finding at the electron microscopy level; it was evident that in the straightened tails, mitochondrial cristae appeared expanse and more electrondense.…”

Oxidative Stress and Male Fertility: Role of Antioxidants and Inositols

“…This led to improvement in outcomes as the fertilization rate, the percentage of grade A embryos on day 3 and progressive motility in normospermic and OAT patients undergoing in vitro fertilization (IVF) [147,148]. In sperm samples from patients with hyper viscosity, MI also improved progressive motility compared to the control group [149]. As the thawing process of sperm samples leads to a reduction in motility, sperm quality and fertilization rate, the efficacy of MI was evaluated on both fresh and thawed sperm samples.…”

New perspectives on male fertility evaluation: Innovative approach for the qualitative analysis of spermatozoa

“…In this context, AT is proposed as a linking bridge between different analytics dimensions, being a functional test, easy to use, with a weak economic burden, that allows a qualitative evaluation of sperm samples analysing myo‐inositol level of spermatic fluids, a fundamental molecule for spermatozoa motility (Artini et al, ; Calogero, Gullo, Vignera, Condorelli, & Vaiarelli, ; Colone et al, ; Condorelli, Vignera, Bellanca, Vicari, & Calogero, ; Condorelli et al, , ). Thanks to AT, it is possible to distinguish three spermatic classes evaluating the modifications of linear progressive motility, a useful parameter to understand the real fertilising capacity of spermatozoa (Scarselli et al, ). Aim of the present study was to evaluate AT potentiality to highlight sperm samples with a good quality, taking as gold standard the analytics capacities of spermiogram and SCD.…”