Analysis of MYO1H Gene Polymorphism in Skeletal Class-III Malocclusion Due to Mandibular Prognathism

Abstract

Background Mandibular prognathism (MP) is a craniofacial deformity resulting from the combined effects of environmental and genetic factors. Although various linkage and genome-wide association studies for mandibular prognathism have identified multiple strongly associated regions and genes, the causal genes and variants responsible for the deformity remained ambiguous. Aim This research work was aimed to study the association between polymorphism rs10850110 of the MYO1H gene and skeletal class-III malocclusion in our local population. Materials and Methods Thirty patients with skeletal class III due to mandibular prognathism in the study group and 30 patients with skeletal class I in the control group were selected for this study. These patients were from both sexes and above age 10 years. Based on the cephalometric values, patients were categorized into study and control groups. SNB (angle between sella, nasion and point B at nasion) greater than 82 degrees with an ANB (angle between point A, nasion and point B at nasion) of less than 0 degrees in the study group and ANB (angle between point A, nasion and point B at nasion) of 2 to 4 degrees in the control group were categorized. The polymorphism (rs10850110) of the MYO1H gene was genotyped using polymerase chain reaction and restriction fragment length polymorphism. Associations were tested with SNP exact test using SNPstats software. Results The single-nucleotide polymorphism rs10850110 showed a statistically significant association with mandibular prognathism. The G allele of marker rs10850110 (5′ of myosin1H - MYO1H) was overrepresented when compared with the “A” allele in mandibular prognathism cases (p < 0.0001), and this was very significant. Conclusion These results suggest that the rs10850110 polymorphism of the MYO1H gene is associated with an increased risk for mandibular prognathism.

Dento-dental volumetric analysis in sagittal skeletal discrepancies: a retrospective observational study

“…Atteeri A. et al demonstrated how the rs10850110 polymorphism of the MYO1H gene has a statistically signi cant association with mandibular prognathism, as the G allele of the rs marker was overrepresented compared to the A ally in cases of mandibular prognathism, concluding that the MYO1H gene is associated with an increased risk of mandibular prognathism 50 .…”

Genetic architecture of non‐syndromic skeletal class III malocclusion

“…However, Dalaie et al found there was no significant correlation between rs10850110 and rs11611277 and the risk of MP in an Iranian population (Dalaie et al, 2020). A recent study analyzed the association between MYO1H rs10850110 and skeletal Class III malocclusion in 30 patients with MP and 30 skeletal Class I malocclusion controls in an Indian population (Atteeri et al, 2021). These results suggest that carrying the G allele of rs10850110 increases the risk of MP.…”

“…Genetic and environmental factors, particularly genetic factors, may influence the development of skeletal Class III malocclusion (Dehesa‐Santos et al, 2021; Grippaudo et al, 2016; Rodríguez‐Olivos et al, 2022; Zhou et al, 2016). Many candidate genes, including MYO1H (Atteeri et al, 2021; Dalaie et al, 2020) , COL2A1 (Dehesa‐Santos et al, 2021), GHR (Tomoyasu et al, 2009), ARHGAP21 (Perillo et al, 2015), and SNAI3 (Dehesa‐Santos et al, 2021) have been reported to be associated with skeletal Class III malocclusion. However, the mechanisms by which these genes are susceptible to skeletal Class III malocclusion remain unclear (Cruz et al, 2011).…”

The association of polymorphisms in BMP2/MYO1H and skeletal Class II div.1 maxillary and mandibular dimensions. A preliminary ‘report

“…Thus, genetic alteration that affects the muscles could affect the skeletal growth ( Moss, 1997 ). Previous studies have linked polymorphisms in MYO1H with mandibular discrepancy in Class III malocclusion ( Da Fontoura et al, 2015 , Wise et al, 2000 , Sun et al, 2018 , Atteeri et al, 2021 ).…”