Analysis of Netrin 1 receptors during inner ear development

Matilainen, Tanja; Haugas, Maarja; Kreidberg, Jordan A.; Salminen, Marjo

doi:10.1387/ijdb.072273tm

Cited by 30 publications

(31 citation statements)

References 19 publications

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“…A similar problem has been described before in the embryonic Ntn1−/− spinal cord where the detachment and/or dispersal of the OPCs from the Ntn1 expressing ventral VZ was inefficient (Jarjour et al, 2003;Tsai et al, 2006). Moreover, NTN1 has a local release function also in inner ear epithelium where the normally Ntn1-expressing cells cannot detach from the underlying basement membrane in Ntn1−/− embryos (Matilainen et al, 2007;Salminen et al, 2000). Similar "short-range" functions for NTN1 have been reported in various tissues where NTN1 mediates cell adhesion/migration and organ morphogenesis (Baker et al, 2006).…”

Section: Ntn1 Is Required For the Efficient Migration Of Ob Progenitosupporting

confidence: 57%

Netrin1 is required for neural and glial precursor migrations into the olfactory bulb

Hakanen

Duprat

Salminen

2011

Developmental Biology

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Netrin1 (NTN1) deficiency in mouse brain causes defects in axon guidance and cell migration during embryonic development. Here we show that NTN1 is required for olfactory bulb (OB) development at late embryogenesis and at early postnatal stages to facilitate the accumulation of proper numbers of granular and glomerular neuron subtypes and oligodendrocytes into the OB. In addition to the analysis of Ntn1-/- mice we made tissue and neurosphere cultures to clarify the role of NTN1 in the anterior forebrain. We propose that a subset of neural progenitors/precursors requires NTN1 to efficiently enter the rostral migratory stream to migrate into the OB. The analysis of postnatal Ntn1-/- OBs revealed a reduction of specific types of interneurons which have been shown to originate from particular subregions of the lateral ventricle walls. Based on Ntn1 expression in ventral parts of the ventricle walls, we observed a decrease in the mainly ventrally derived type II interneurons that express calcium-binding proteins calretinin and calbindin. Instead, no change in the numbers of dorsally derived tyrosine hydroxylase expressing interneurons was detected. In addition to the specific reduction of type II interneurons, our results indicate that NTN1 is required for oligodendroglial migration into the OB. Furthermore, we characterised the Ntn1 expressing subpopulation of neurosphere-forming cells from embryonic and adult brain as multipotent and self-renewing. However, NTN1 is dispensable for the proliferation of neurosphere forming progenitor cells and for their differentiation.

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Section: Ntn1 Is Required For the Efficient Migration Of Ob Progenitosupporting

confidence: 57%

Netrin1 is required for neural and glial precursor migrations into the olfactory bulb

Hakanen

Duprat

Salminen

2011

Developmental Biology

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“…Nevertheless, inactivation of these genes causes defects only in the cochlear compartment and moreover, no problems in the mesenchymal clearance was reported (Phippard et al, 1999;Trowe et al, 2008). We also verified the state of perilymphatic space maturation in three lines of knock-out embryos, where either Neogenin (Neo1), Unc5b, or Unc5c genes, known to be expressed in periotic mesenchyme during ear morphogenesis, have been inactivated (Ackerman et al, 1997;Lu et al, 2004;Matilainen et al, 2007). No clearance defect could be observed in these embryos at E16.5-E18.5 (data not shown).…”

Section: Gata2 Is Required For the Formation Of The Perilymphatic Spacesupporting

confidence: 53%

Gata2 is required for the development of inner ear semicircular ducts and the surrounding perilymphatic space

Haugas¹,

Lilleväli²,

Hakanen³

et al. 2010

Developmental Dynamics

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*Gata2 has essential roles in the development of many organs. During mouse inner ear morphogenesis, it is expressed in otic vesicle and the surrounding periotic mesenchyme from early on, but no defects in the ear development of Gata2 null mice have been observed before lethality at embryonic day (E) 10.5. Here, we used conditional gene targeting to reveal the role of Gata2 at later stages of inner ear development. We show that Gata2 is critically required from E14.5-E15.5 onward for vestibular morphogenesis. Without Gata2 the semicircular ducts fail to grow to their normal size and the surrounding mesenchymal cells are not removed properly to generate the perilymphatic space. Gata2 is the first factor known to control the clearing of the vestibular perilymphatic mesenchyme, but interestingly, it is not required for the formation of the cochlear perilymphatic areas, suggesting distinct molecular control for these processes.

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“…1F′ and G). In E11.5 and E12 mouse embryo, Itgb1 was detected to express in the otic epithelium, suggesting its conservative involvement in ear development (Matilainen et al, 2007). In addition, itgb1a transcripts were localized in heart at 72 hpf (Fig.…”

Section: Expression Patterns Of Itgb1a Mrna During Developmentmentioning

confidence: 89%

Expression analysis of integrin β1 isoforms during zebrafish embryonic development

Wang

Liu

2014

Gene Expression Patterns

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Analysis of Netrin 1 receptors during inner ear development

Cited by 30 publications

References 19 publications

Netrin1 is required for neural and glial precursor migrations into the olfactory bulb

Netrin1 is required for neural and glial precursor migrations into the olfactory bulb

Gata2 is required for the development of inner ear semicircular ducts and the surrounding perilymphatic space

Expression analysis of integrin β1 isoforms during zebrafish embryonic development

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