Analysis of neurotransmitters and their metabolites by liquid chromatography

Bosse, Kelly E.; Birbeck, Johnna A.; Newman, Brooke D.; Mathews, Tiffany A.

doi:10.1016/b978-0-12-805392-8.00021-9

Cited by 1 publication

(1 citation statement)

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“…As a consequence, numerous methods have been reported for the quantification of biogenic monoamines and their respective metabolites, including colorimetric and fluorometric methods (Udenfriend et al , ; Hardeman et al , ), radioimmunoassays (Engbaek and Voldby, ; Francis et al , ) or enzyme‐linked‐immunosorbent assays (Westermann et al , ; Park and Paeng, ). Owing to major advances in chromatography‐based methods and column technologies, multianalyte detection capability has been greatly enhanced (Bosse et al , ) and high‐performance liquid chromatography (HPLC) is now widely used for separation of such coumpounds. Since most biogenic monoamines can be oxidized, HPLC coupled with electrochemical detection (HPLC‐ECD) is one of the screening methods most frequently used (Church, ), even though HPLC coupled with mass spectrometry or fluorescence detection is also used (Umeda et al , ; González et al , ; De Benedetto et al , ).…”

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confidence: 99%

An HPLC‐ECD method for monoamines and metabolites quantification in cuttlefish (cephalopod) brain tissue

Bidel

Corvaisier

Jozet‐Alves

et al. 2016

Biomedical Chromatography

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The cuttlefish belongs to the mollusk class Cephalopoda, considered as the most advanced marine invertebrates and thus widely used as models to study the biology of complex behaviors and cognition, as well as their related neurochemical mechanisms. Surprisingly, methods to quantify the biogenic monoamines and their metabolites in cuttlefish brain remain sparse and measure a limited number of analytes. This work aims to validate an HPLC-ECD method for the simultaneous quantification of dopamine, serotonin, norepinephrine and their main metabolites in cuttlefish brain. In comparison and in order to develop a method suitable to answer both ecological and biomedical questions, the validation was also carried out on a phylogenetically remote species: mouse (mammals). The method was shown to be accurate, precise, selective, repeatable and sensitive over a wide range of concentrations for 5-hydroxyindole-3-acetic acid, serotonin, dopamine, 3,4-dihydroxyphenylacetic acid and norepinephrine in the both extracts of cuttlefish and mouse brain, though with low precision and recovery for 4-hydroxy-3-methoxyphenylethylene glycol. Homovanillic acid, accurately studied in rodents, was not detectable in the brain of cuttlefish. Overall, we described here the first fully validated HPLC method for the routine measurement of both monoamines and metabolites in cuttlefish brain. Copyright © 2016 John Wiley & Sons, Ltd.

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