Analysis of nickel species in cytosols of normal and malignant human colonic tissues using two dimensional liquid chromatography with ICP-sector field MS detection

Bouyssière, Brice; Knispel, Tina; Ruhnau, Christiane; Denkhaus, Evelin; Prange, Andreas

doi:10.1039/b308607k

Cited by 12 publications

(10 citation statements)

References 32 publications

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“…Exposure to 1 mM Cd triggered a transient growth arrest. Consequently, we determined, by Inductively Coupled Plasma Mass Spectroscopy (ICP-MS, [30] , [31] ), the amount of Cd that exponentially growing cells retain at 30 min, 120 min, 270 min and 24 hrs under our culture conditions (ASW-AA-Cyst medium) and showed that cells always retain several percents of the amount of Cd (1 mM) added to the culture ( Table S1 ). However, these percentages differ from one culture to another, except for the 24 hrs timepoint.…”

Section: Resultsmentioning

confidence: 99%

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Genome-Wide Transcriptional Response of the Archaeon Thermococcus gammatolerans to Cadmium

et al. 2012

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Thermococcus gammatolerans, the most radioresistant archaeon known to date, is an anaerobic and hyperthermophilic sulfur-reducing organism living in deep-sea hydrothermal vents. Knowledge of mechanisms underlying archaeal metal tolerance in such metal-rich ecosystem is still poorly documented. We showed that T. gammatolerans exhibits high resistance to cadmium (Cd), cobalt (Co) and zinc (Zn), a weaker tolerance to nickel (Ni), copper (Cu) and arsenate (AsO4) and that cells exposed to 1 mM Cd exhibit a cellular Cd concentration of 67 µM. A time-dependent transcriptomic analysis using microarrays was performed at a non-toxic (100 µM) and a toxic (1 mM) Cd dose. The reliability of microarray data was strengthened by real time RT-PCR validations. Altogether, 114 Cd responsive genes were revealed and a substantial subset of genes is related to metal homeostasis, drug detoxification, re-oxidization of cofactors and ATP production. This first genome-wide expression profiling study of archaeal cells challenged with Cd showed that T. gammatolerans withstands induced stress through pathways observed in both prokaryotes and eukaryotes but also through new and original strategies. T. gammatolerans cells challenged with 1 mM Cd basically promote: 1) the induction of several transporter/permease encoding genes, probably to detoxify the cell; 2) the upregulation of Fe transporters encoding genes to likely compensate Cd damages in iron-containing proteins; 3) the induction of membrane-bound hydrogenase (Mbh) and membrane-bound hydrogenlyase (Mhy2) subunits encoding genes involved in recycling reduced cofactors and/or in proton translocation for energy production. By contrast to other organisms, redox homeostasis genes appear constitutively expressed and only a few genes encoding DNA repair proteins are regulated. We compared the expression of 27 Cd responsive genes in other stress conditions (Zn, Ni, heat shock, γ-rays), and showed that the Cd transcriptional pattern is comparable to other metal stress transcriptional responses (Cd, Zn, Ni) but not to a general stress response.

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Section: Resultsmentioning

confidence: 99%

“…The solution was diluted 1000 times in HNO 3 2% prior analysis by ICP-MS. The washing supernatants were diluted 10 times in HNO 3 2% prior analysis by ICP-MS. Rhodium was used as internal standard and quantifications were performed by external calibration [30] , [31] (Agilent 7500 ICP-MS, Agilent Technologies, Tokyo, Japan).…”

Section: Methodsmentioning

confidence: 99%

Genome-Wide Transcriptional Response of the Archaeon Thermococcus gammatolerans to Cadmium

et al. 2012

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“…199 Bidimensional AE-SEC-HR ICP MS allowed the resolution of 7 Ni species in tissues from cancer patients but no difference in binding patterns between cytosols of normal and malignant colon tissues could be revealed. 206 Reversed-phase HPLC-ICP MS was used for the analysis for metal-MT complexes in rat liver and kidney. 207 Capillary electrophoresis offers a better separation efficiency of MT isoforms and their complexes than HPLC.…”

Section: Multidimensional Chromatography With Icp Ms Detectionmentioning

confidence: 99%

Advances in analytical methodology for bioinorganic speciation analysis: metallomics, metalloproteomics and heteroatom-tagged proteomics and metabolomics

Szpunar

2005

Analyst

379

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The recent developments in analytical techniques capable of providing information on the identity and quantity of heteroatom-containing biomolecules are critically discussed. Particular attention is paid to the emerging areas of bioinorganic analysis including: (i) a comprehensive analysis of the entirety of metal and metalloid species within a cell or tissue type (metallomics), (ii) the study of the part of the metallome involving the protein ligands (metalloproteomics), and (iii) the use of a heteroelement, naturally present in a protein or introduced in a tag added by means of derivatisation, for the spotting and quantification of proteins (heteroatom-tagged proteomics). Inductively coupled plasma mass spectrometry (ICP MS), used as detector in chromatography and electrophoresis, and supported by electrospray and MALDI MS, appears as the linchpin analytical technique for these emerging areas. This review focuses on the recent advances in ICP MS in biological speciation analysis including sensitive detection of non-metals, especially of sulfur and phosphorus, couplings to capillary and nanoflow HPLC and capillary electrophoresis, laser ablation ICP MS detection of proteins in gel electrophoresis, and isotope dilution quantification of biomolecules. The paper can be considered as a followup of a previous review by the author on a similar topic (J. Szpunar, Analyst, 2000, 125, 963).

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“…SEC‐AE HPLC‐ICP MS and stable‐isotope labeling were used to study the Cu turnover in marine animals (Mason & Borja, 2002). Bidimensional AE‐SEC‐HR ICP MS allowed the resolution of seven Ni species in tissues from cancer patients, but no difference in binding patterns between cytosols of normal and malignant colon tissues could be revealed (Bouyssiere et al, 2004). Reversed‐phase HPLC‐ICP MS was used for the analysis for metal‐MT complexes in rat liver and kidney (Polec et al, 2002).…”

Section: Applications: Heteoroatom‐containing Proteinsmentioning

confidence: 99%

Mass spectrometry in bioinorganic analytical chemistry

Łobiński

Schaumlöffel

Szpunar

2005

Mass Spectrometry Reviews

190

158

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A considerable momentum has recently been gained by in vitro and in vivo studies of interactions of trace elements in biomolecules due to advances in inductively coupled plasma mass spectrometry (ICP MS) used as a detector in chromatography and capillary and planar electrophoresis. The multiisotopic (including non-metals such as S, P, or Se) detection capability, high sensitivity, tolerance to matrix, and large linearity range regardless of the chemical environment of an analyte make ICP MS a valuable complementary technique to electrospray MS and MALDI MS. This review covers different facets of the recent progress in metal speciation in biochemistry, including probing in vitro interactions between metals and biomolecules, detection, determination, and structural characterization of heteroatom-containing molecules in biological tissues, and protein monitoring and quantification via a heteroelement (S, Se, or P) signal. The application areas include environmental chemistry, plant and animal biochemistry, nutrition, and medicine. #

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Analysis of nickel species in cytosols of normal and malignant human colonic tissues using two dimensional liquid chromatography with ICP-sector field MS detection

Cited by 12 publications

References 32 publications

Genome-Wide Transcriptional Response of the Archaeon Thermococcus gammatolerans to Cadmium

Genome-Wide Transcriptional Response of the Archaeon Thermococcus gammatolerans to Cadmium

Advances in analytical methodology for bioinorganic speciation analysis: metallomics, metalloproteomics and heteroatom-tagged proteomics and metabolomics

Mass spectrometry in bioinorganic analytical chemistry

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