Analysis of nitric oxide synthase gene polymorphisms in neonatal respiratory distress syndrome among the Chinese Han population

Abstract

AimTo evaluate the association of NOS1 and NOS3 gene polymorphisms with the risk/severity of neonatal respiratory distress syndrome (RDS) among preterm infants.MethodsThe patient group was 189 preterm infants diagnosed with RDS. The control group was 227 preterm neonates who did not develop RDS. NOS genotyping was performed using an improved multiplex ligation detection reaction (iMLDR) technique based on LDR.ResultsIt was found that genotype and allele frequencies of rs2682826 of the NOS1 gene and rs1799983 of the NOS3 gene were not significantly different between the RDS group and the control group. However, when the preterm infants were divided into two and three groups based on gestational age and birth weight, a study of the SNP rs1799983 of the NOS3 gene showed that the GG genotype and G allele frequencies were significantly increased in the RDS groups, the GT genotype and A allele were less frequent among the RDS groups in 26–32.9 weeks of gestational age and in a birth weight subgroup of <1.5 Kg.ConclusionOur study raises the possibility that a genetic variation of NOS3 could be implicated in the pathophysiology of RDS in the Chinese Han population, especially in very preterm and very low birth weight infants.

Association of Genetic Variation of CIITA and NTCP with Chronic Hepatitis B Virus Infection in Han Chinese Populations

“…The SNPs genotyping work was performed using an improved multiplex ligation detection reaction (iMLDR) technique (12), which was recently developed by Genesky Biotechnologies Inc. (Shanghai, China). The iMLDR is an improved multiplex SNP discrimination technology based on the traditional ligation detection reaction (LDR).…”

Association between functional polymorphisms in the nitric oxide synthase 3 gene and pediatric acute respiratory distress syndrome

“…The results of this study also implicated the Nox2-mediated eNOS-S-glutathionylation in LPS-induced eNOS uncoupling in the lung microvasculature (Wu et al, 2014). A study of the SNP rs1799983 in NOS3 showed a significant increase in the GG genotype and G allele frequencies in groups with respiratory distress syndrome (Shen et al, 2014). Additional studies must be conducted to determine if functional SNPs in NOS3 modulate the risk of disease by themselves or by correlating with other causative SNPs, and their effect on other populations.…”

Endothelial nitric oxide synthase gene glu298asp polymorphism in preterm neonates with respiratory distress syndrome

“…12 Moreover, Shen et al observed nonsignificant increased GG genotype and G allele among RDS neonates in Chinese. 13 However, Sivasli et al found no association with neonatal RDS among Turkish. 14 Present data demonstrate that T allele is independent risk factor for neonatal RDS.…”