Analysis of nitrite and nitrate in biological samples using high-performance liquid chromatography

Jobgen, Wenjuan S.; Jobgen, Scott C.; Li, Hui; Meininger, Cynthia J.; Wu, Guoyao

doi:10.1016/j.jchromb.2006.07.018

Cited by 145 publications

(83 citation statements)

References 71 publications

(146 reference statements)

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“…Chemiluminescence analyses and Griess colorimetric assays are the most commonly used methods, but the chemiluminescence analysis shows certain disadvantages, such as bulky apparatus, low specificity and sensitivity, and inability to directly detect NO 3 − . Although the Griess reaction is simple, this sample amounts low sensitivity and specificity (Jobgen et al 2007;Hetrick and Schoenfisch 2009). …”

Section: Discussionmentioning

confidence: 99%

“…The limit of detection of the present HPLC method for NO 2 − and NO 3 − analyses is much higher than that of the Griess colorimetric assay (limit of detection of 2 μmol.L −1 ) (Jobgen et al 2007). The limits of detection of the chemiluminescence method for NO 2 − and NO 3 − have been reported to be approximately 1.0 × 10 6 μmol.L −1 and 8.0×10 5 μmol.L −1 , respectively (Tsikas 2007).…”

Section: Limit Of Detectionmentioning

confidence: 99%

“…These techniques include the Griess colorimetric assay, electrochemical detection, chemiluminescence, and capillary electrophoresis (Jimidar et al 1995;Cheng and Tsang 1998;Siu andHenshall 1998, Tsikas 2007). However, these methods present disadvantages (Jobgen et al 2007). …”

Section: Introductionmentioning

confidence: 99%

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Quantitative and Comparative Contents of Nitrate and Nitrite in Beta vulgaris L. by Reversed-Phase High-Performance Liquid Chromatography-Fluorescence

et al. 2015

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The consumption of beetroot, a food rich in nitrate, has been recommended to enhance blood perfusion, restore endothelial function, and improve exercise performance. These properties may be explained by the possible effect of nitrate present in beetroot in stimulating the endogenous synthesis of nitric oxide, a potent vasodilator. However, there is limited evidence giving detailed information about how nitrate contents from beetroot used in studies have been analyzed. The purpose of this study was to evaluate nitrate and nitrite contents of beetroot from different regions of Brazil and from the USA. Nitrate and nitrite contents were quantified by using a reversed-phase high-performance liquid chromatography system with fluorescence detection. Beetroots from the USA showed the highest nitrate (31.2±0.010 mmol.L −1 ) and nitrite (0.45±0.005 mmol.L −1 ) contents when compared to beetroots from Brazil. Rio de Janeiro was the region that showed the highest nitrate content (17.1±0.020 mmol.L −1 ), while Rio Grande do Norte presented the highest nitrite content (0.13±0.010 mmol.L −1 ). The reversed-phase high-performance liquid chromatography-fluorescence method may be applied for the quantification of nitrate and nitrite contents in beetroot samples. The results of the present study demonstrate that beetroots may vary in their nitrate and nitrite contents.This data represents a useful tool to encourage researchers to analyze the nitrate and nitrite contents in beetroots utilized in interventional clinical studies.

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Section: Discussionmentioning

confidence: 99%

Section: Limit Of Detectionmentioning

confidence: 99%

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Quantitative and Comparative Contents of Nitrate and Nitrite in Beta vulgaris L. by Reversed-Phase High-Performance Liquid Chromatography-Fluorescence

et al. 2015

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“…In addition, these separation techniques become increasingly accessible in regular biological and medical laboratories. HPLC analysis with focus on nitrite and nitrate in biological samples is subject of another review [227]. The present review focuses on the current status and trends in the development of HPCE applications for profiling the NO pathway with some emphasis on applications of various CE approaches in biomedical and life sciences.…”

Section: Analytical Approaches To Study No Metabolitesmentioning

confidence: 99%

Bioanalytical profile of the l-arginine/nitric oxide pathway and its evaluation by capillary electrophoresis

Boudko

2007

Journal of Chromatography B

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This review briefly summarizes recent progress in fundamental understanding and analytical profiling of the L-arginine/nitric oxide (NO) pathway. It focuses on key analytical references of NO actions and on the experimental acquisition of these references in vivo, with capillary electrophoresis (CE) and high-performance capillary electrophoresis (HPCE) comprising one of the most flexible and technologically promising analytical platform for comprehensive high-resolution profiling of NO-related metabolites. Second aim of this review is to express demands and bridge efforts of experimental biologists, medical professionals and chemical analysis-oriented scientists who strive to understand evolution and physiological roles of NO and to develop analytical methods for use in biology and medicine.

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“…This approach, however, does not work for nitrite and requires the use of strong acid conditions which may be critical if any nitrite is present because of its possible conversion to nitrate (5)(6) . Another technique used with GC-MS entails alkylation with pentafluorobenzyl bromide (F 5 BzBr) (7) to convert nitrite and nitrate to F 5 Bz-NO 2 and F 5 Bz-ONO 2 , respectively. The pentafluorobenzyl derivates are suitable for negative chemical ionization GC-MS, but they are not been employed to perform headspace analysis likely for their low volatility.…”

Section: Introductionmentioning

confidence: 99%

Negative Chemical Ionization GC/MS Determination of Nitrite and Nitrate in Seawater Using Exact Matching Double Spike Isotope Dilution and Derivatization with Triethyloxonium Tetrafluoroborate

et al. 2012

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Access and use of this website and the material on it are subject to the Terms and Conditions set forth at Vous avez des questions? Nous pouvons vous aider. Pour communiquer directement avec un auteur, consultez la première page de la revue dans laquelle son article a été publié afin de trouver ses coordonnées. Si vous n'arrivez pas à les repérer, communiquez avec nous à PublicationsArchive-ArchivesPublications@nrc-cnrc.gc.ca. Questions? Contact the NRC Publications Archive team atPublicationsArchive-ArchivesPublications@nrc-cnrc.gc.ca. If you wish to email the authors directly, please see the first page of the publication for their contact information. NRC Publications Archive Archives des publications du CNRCThis publication could be one of several versions: author's original, accepted manuscript or the publisher's version. / La version de cette publication peut être l'une des suivantes : la version prépublication de l'auteur, la version acceptée du manuscrit ou la version de l'éditeur. For the publisher's version, please access the DOI link below./ Pour consulter la version de l'éditeur, utilisez le lien DOI ci-dessous.http://doi.org/10.1021/ac2030128Analytical Chemistry, 84, 5, pp. 2592-2596, 2012-03-06 A BACBD EF D A ABSTRACTThe alkylation of nitrite and nitrate by triethyloxonium tetrafluoroborate allows determination of their ethyl esters by headspace GC-MS. In the present study, significant improvement in analytical performance are achieved by using negative chemical ionization (CI-). Detection limits are improved for at least an order of magnitude than those achieved using electron impact ionization (EI). The derivatization procedure has been optimized and alkaline conditions are adopted to minimize the conversion of nitrite to nitrate (determined to be 0.07% at 100 mg/L NO 2 -) and to avoid the exchange of oxygen between the analytes and the solvent (water).Quantitation entails the use of isotopically enriched standards (N 18 O 2 -and 15 NO 3 -), which also permits monitoring of potential conversion from nitrite to nitrate during the analysis (double spike isotope dilution).

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Analysis of nitrite and nitrate in biological samples using high-performance liquid chromatography

Cited by 145 publications

References 71 publications

Quantitative and Comparative Contents of Nitrate and Nitrite in Beta vulgaris L. by Reversed-Phase High-Performance Liquid Chromatography-Fluorescence

Quantitative and Comparative Contents of Nitrate and Nitrite in Beta vulgaris L. by Reversed-Phase High-Performance Liquid Chromatography-Fluorescence

Bioanalytical profile of the l-arginine/nitric oxide pathway and its evaluation by capillary electrophoresis

Negative Chemical Ionization GC/MS Determination of Nitrite and Nitrate in Seawater Using Exact Matching Double Spike Isotope Dilution and Derivatization with Triethyloxonium Tetrafluoroborate

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