Analysis of Non-volatile Constituents in Dracocephalum Species by HPLC and GC-MS

Kakasy, A.; Füzfai, Zs.; Kursinszki, L.; Molnár-Perl, I.; Lemberkovics, Éva

doi:10.1365/s10337-006-0741-x

Cited by 25 publications

(13 citation statements)

References 28 publications

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“…In the literature, there are also reports on the presence of additional phenolic compounds in D. moldavica plants, which, besides RA, can be responsible for the observed antioxidant activity. These include caffeic acid, chlorogenic acid (Kakasy et al 2006), phenylpropanoids and flavonoids (Sultan et al 2008). To identify the compounds in hairy roots of D. moldavica, further phytochemical analyses are needed.…”

Section: Resultsmentioning

confidence: 99%

“…There are also reports on anti-Helicobacter pylori activity (Ghanndi et al 2004) as well as sedative, analgesic and wound-healing properties of dragonhead extracts (Sultan et al 2008). Crude extracts of the aerial parts of D. moldavica contain a number of constituents with well-documented biological activity, including flavonoids (luteolin, 7-O-glucoside luteolin, apigenin), iridoids, essential oil components, ursolic and oleanolic acids as well as hydroxycinnamic acids with caffeic, ferulic and rosmarinic acids (Kakasy et al 2006;Popova et al 2008;Sultan et al 2008).…”

Section: Introductionmentioning

confidence: 99%

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Hairy roots of Dracocephalum moldavica: rosmarinic acid content and antioxidant potential

et al. 2013

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Hairy roots of Dracocephalum moldavica L. were induced using Agrobacterium rhizogenes strain A4. Transformed roots were obtained from shoot explants with low transformation frequency of up to 3 %. The effects of different liquid media: Murashige and Skoog (MS), Gamborg et al. (B5) and Woody Plant (WP) with full-and half-strength ( MS, B5, WP), on biomass accumulation and rosmarinic acid (RA) content were investigated. The hairy roots were cultured in photoperiod (16 h light/8 h dark) and darkness. Biomass of D. moldavica hairy roots was the highest (7.23 g flask -1 of fresh weight and 0.89 g flask -1 of dry weight) in the cultures grown in WP medium under periodic light. Ultra performance liquid chromatography analysis revealed the highest RA content (78 mg g -1 dry wt) in roots cultured in B5 medium under photoperiod conditions. It was about tenfold higher compared to roots of field-grown mother plants. Antioxidant activities and total phenolic contents of methanolic extracts of D. moldavica hairy roots cultured in B5 and WP media under photoperiod and darkness and roots of field grown plants were compared. All extracts were investigated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging and phosphomolybdenum reduction assays. Total phenolic contents were estimated by the FolinCiocalteu method. The methanolic extract of D. moldavica hairy roots grown in B5 medium under photoperiod possessed the strongest effects on reducing Mo and DPPH radical scavenging. The activities were significantly higher (p B 0.05) than those of methanolic extract of roots of intact plants grown in the field. The most active methanolic extract of hairy roots was characterized by the highest level of rosmarinic acid and total content of phenolic compounds.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Hairy roots of Dracocephalum moldavica: rosmarinic acid content and antioxidant potential

et al. 2013

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“…Li et al (2009a, b) identified more than sixty compounds belonging to different chemical groups in ethanolic extracts from whole plants of D. forrestii. Most compounds were monoterpenes, but the authors also described caffeic acid derivatives such as caffeic acid and methyl rosmarinate: the former being earlier described in D. moldavica (Kakasy et al 2006), D. peregrinum (Dai et al 2008) and D. ruyschiana (Kakasy et al 2006), and the latter in aerial parts of D. moldavica (Yang et al 2013). However, our work is the first report to describe the presence of salvianolic acids such as B, I/H, E and lithospermic acid A in the shoots of D. forrestii.…”

Section: Identification Of Phenolic Compounds Of the Hydromethanolic mentioning

confidence: 99%

Effect of cytokinins on shoots proliferation and rosmarinic and salvianolic acid B production in shoot culture of Dracocephalum forrestii W. W. Smith

et al. 2018

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The current study estimates the effect of different cytokinins on shoot proliferation and biosynthesis of caffeic acid derivatives in Dracocephalum forrestii in vitro culture. The shoots were grown on Murashige and Skoog (MS) agar medium with 1 µM indole-3-acetic acid (IAA) and different content of 6-benzyloaminopurine (BAP), zeatin, kinetin (1, 2, 4, 8, 18 µM) or thidiazuron (TDZ) (0.1, 0.2, 0.5, 1, 2 µM). The highest multiplication rate (about seven shoots and/or buds per explant) was obtained after 4 weeks of culture on MS medium with 1 µM IAA and 8 or 16 µM BAP. Optimal biomass of plant material was also received on the same media. The identity of the compounds present in the hydromethanolic extracts from D. forrestii shoots grown on cytokinin-supplemented media was confirmed using UPLC-PDA-ESI-MS method. The analysis revealed the presence of nine metabolites recognized as caffeic acid derivatives. The content of the predominant phenolic acids in the extracts, i.e. rosmarinic acid (RA) and salvianolic acid B (SAB), was determined with UHPLC. The highest yield of RA was found in shoots cultivated in the medium containing 1 µM IAA and 2 µM BAP (18.7 mg/g DW). The highest level of SAB (5.3-5.9 mg/g DW) was identified in multiple shoots grown in the presence of 1 µM IAA and 0.5-1 µM TDZ or 2 µM BAP.

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“…This plant is native to Siberian and Central Asia, it also grows in Egypt, China and Mongolia at altitudes of up to 2700-3100 m above sea level . D. moldavica is naturalized in Eastern and Central Europe (Kakasy et al, 2006). Its aerial parts have been widely utilized in traditional medicine for the treatment of stomach and liver disorders as well as headache and toothache (Dastmalchi et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

“…Studies indicate that D. moldavica extracts have anti-Helicobacter pylori activity (Ghannadi et al, 2004) as well as sedative, analgesic and wound-healing properties (Sultan et al, 2008). The main substances responsible for the therapeutic effects of the D. moldavica aerial parts are phenolic acids (mainly rosmarinic, caffeic and ferulic acids), flavonoids and essential oil components (Kakasy et al, 2006;Popova et al, 2008;Sultan et al, 2008). Flavonoids and phenolic acids are known to be beneficial to human health and disease prevention thanks to their strong antioxidant activity, capacity to scavenge free radicals and ability to neutralize reactive oxygen species (Saxena et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Rosmarinic Acid Accumulation and Antioxidant Potential of Dracocephalum moldavica L. Cell Suspension Culture

Weremczuk-Jeżyna

Grzegorczyk-Karolak

Frydrych

et al. 2017

Not Bot Horti Agrobo

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Dracocephalum moldavica L. (Lamiaceae) is known for its medicinal properties; however greater yields can potentially be achieved by in vitro cultivation. A cell suspension culture of D. moldavica L. (Lamiaceae) derived from root-derived callus was established in liquid MS medium supplemented with 2,4-D 0.5 mg/l and BAP 0.2 mg/l. The biomass and rosmarinic acid (RA) content were analyzed during the 15-day growth cycle of the culture. The highest fresh and dry weight (14.29 g/flask and 1.14 g/flask, respectively) and RA level (27.2 mg/g DW) were reached at day 12 of culture. Methanolic extracts of the culture were assayed for total phenolic content using the Folin-Ciocalteau method, and antioxidant activities using three in vitro tests: ABTS radical scavenging, ferric ion reduction (FRAP) and lipid peroxidation (LPO). RA content and antioxidant potential were found to be higher in cell suspension culture than in root-derived callus. The cell suspension culture also exhibited higher concentrations of RA and ABTS radical scavenging activity than those of the aerial parts of six-month-old field-grown plants of D. moldavica. The overall results show a significant correlation between antioxidant activity, total phenolic content and RA content of the examined extracts. The study presents for the first time the use of cell cultures of D. moldavica for production of therapeutically-valuable metabolites. Our results suggest that the obtained culture could be considered as a potential source of rosmarinic acid, a compound known for its strong antioxidant activity.

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Analysis of Non-volatile Constituents in Dracocephalum Species by HPLC and GC-MS

Cited by 25 publications

References 28 publications

Hairy roots of Dracocephalum moldavica: rosmarinic acid content and antioxidant potential

Hairy roots of Dracocephalum moldavica: rosmarinic acid content and antioxidant potential

Effect of cytokinins on shoots proliferation and rosmarinic and salvianolic acid B production in shoot culture of Dracocephalum forrestii W. W. Smith

Rosmarinic Acid Accumulation and Antioxidant Potential of Dracocephalum moldavica L. Cell Suspension Culture

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