Analysis of odorant-binding protein gene family members in the polyembryonic wasp, Copidosoma floridanum: Evidence for caste bias and host interaction

Donnell, David M.

doi:10.1016/j.jinsphys.2013.12.002

Cited by 10 publications

(6 citation statements)

References 54 publications

(64 reference statements)

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“…A few fragments were methylated in one caste but not in the other. This is compatible with the finding that six odorant-binding protein genes are differentially expressed in soldiers vs. reproductives of Copidosoma floridanum (Donnell 2014). A similar picture was found in honeybees, where about 10% of the methylated genes showed significant differences in the extent of methylation between queens and workers (Lyko et al 2010).…”

Section: Discussionsupporting

confidence: 89%

Genome methylation patterns across castes and generations in a parasitoid wasp

Shaham

Ben‐Shlomo

Motro

et al. 2016

Ecology and Evolution

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Environmental influences shape phenotypes within and across generations, often through DNA methylations that modify gene expression. Methylations were proposed to mediate caste and task allocation in some eusocial insects, but how an insect's environment affects DNA methylation in its offspring is yet unknown. We characterized parental effects on methylation profiles in the polyembryonic parasitoid wasp Copidosoma koehleri, as well as methylation patterns associated with its simple caste system. We used methylation‐sensitive amplified fragment length polymorphism (MS‐AFLP) to compare methylation patterns, among (1) reproductive and soldier larvae; and (2) offspring (larvae, pupae, and adults) of wasps that were reared at either high or low larval density and mated in the four possible combinations. Methylation frequencies were similar across castes, but the profiles of methylated fragments differed significantly. Parental rearing density did not affect methylation frequencies in the offspring at any developmental stage. Principal coordinate analysis indicated no significant differences in methylation profiles among the four crossbreeding groups and the three developmental stages. Nevertheless, a clustering analysis, performed on a subset of the fragments, revealed similar methylation patterns in larvae, pupae, and adults in two of the four parental crosses. Nine fragments were methylated at two cytosine sites in all larvae, and five others were methylated at two sites in all adults. Thus, DNA methylations correlate with within‐generation phenotypic plasticity due to caste. However, their association with developmental stage and with transgenerational epigenetic effects is not clearly supported.

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Section: Discussionsupporting

confidence: 89%

Genome methylation patterns across castes and generations in a parasitoid wasp

Shaham

Ben‐Shlomo

Motro

et al. 2016

Ecology and Evolution

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“…It has been reported that OBP genes in another polyembryonic wasp C. floridanum showed caste-specific functions. The majority of OBP genes might have the functions in contacting with host hemolymph [ 29 ]. The decrease of P450 and GSTs gene families suggests the detoxification ability of the parasitoid wasp has degenerated, again perhaps because the host protects the parasitoid larvae from the external environment.…”

Section: Resultsmentioning

confidence: 99%

The genomic features of parasitism, Polyembryony and immune evasion in the endoparasitic wasp Macrocentrus cingulum

Yin

et al. 2018

BMC Genomics

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BackgroundParasitoid wasps are well-known natural enemies of major agricultural pests and arthropod borne diseases. The parasitoid wasp Macrocentrus cingulum (Hymenoptera: Braconidae) has been widely used to control the notorious insect pests Ostrinia furnacalis (Asian Corn Borer) and O. nubilalis (European corn borer). One striking phenomenon exhibited by M. cingulum is polyembryony, the formation of multiple genetically identical offspring from a single zygote. Moreover, M. cingulum employs a passive parasitic strategy by preventing the host’s immune system from recognizing the embryo as a foreign body. Thus, the embryos evade the host’s immune system and are not encapsulated by host hemocytes. Unfortunately, the mechanism of both polyembryony and immune evasion remains largely unknown.ResultsWe report the genome of the parasitoid wasp M. cingulum. Comparative genomics analysis of M. cingulum and other 11 insects were conducted, finding some gene families with apparent expansion or contraction which might be linked to the parasitic behaviors or polyembryony of M. cingulum. Moreover, we present the evidence that the microRNA miR-14b regulates the polyembryonic development of M. cingulum by targeting the c-Myc Promoter-binding Protein 1 (MBP-1), histone-lysine N-methyltransferase 2E (KMT2E) and segmentation protein Runt. In addition, Hemomucin, an O-glycosylated transmembrane protein, protects the endoparasitoid wasp larvae from being encapsulated by host hemocytes. Motif and domain analysis showed that only the hemomucin in two endoparasitoids, M. cingulum and Venturia canescens, possessing the ability of passive immune evasion has intact mucin domain and similar O-glycosylation patterns, indicating that the hemomucin is a key factor modulating the immune evasion.ConclusionsThe microRNA miR-14b participates in the regulation of polyembryonic development, and the O-glycosylation of the mucin domain in the hemomucin confers the passive immune evasion in this wasp. These key findings provide new insights into the polyembryony and immune evasion.Electronic supplementary materialThe online version of this article (10.1186/s12864-018-4783-x) contains supplementary material, which is available to authorized users.

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“…The abundance of OBP genes identified in A. bambawalei is relatively higher than that in other hymenopteran insects. Only eight and 12 putative OBP genes have been identified in A. hispinarum and C. floridanum , respectively (Donnell, ). In addition, there are only 10 predicted OBP genes in Sclerodermus sp.…”

Section: Discussionmentioning

confidence: 99%

Antennal transcriptome and odorant binding protein expression profiles of an invasive mealybug and its parasitoid

Nie

et al. 2017

J Applied Entomology

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Olfaction is fundamental to insect survival due to its roles in host location and interspecific communications. Here, we analysed the transcriptomes of odorant binding proteins (OBPs) from the invasive mealybug Phenacoccus solenopsis and its endopara- AbamOBP30 were expressed at high levels in male antenna. This study for the first time identified the chemosensory genes and determined their expression patterns in different tissues of the two species. Our results provide important molecular information for the exploration of chemosensory mechanisms in these two species.

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Analysis of odorant-binding protein gene family members in the polyembryonic wasp, Copidosoma floridanum: Evidence for caste bias and host interaction

Cited by 10 publications

References 54 publications

Genome methylation patterns across castes and generations in a parasitoid wasp

Genome methylation patterns across castes and generations in a parasitoid wasp

The genomic features of parasitism, Polyembryony and immune evasion in the endoparasitic wasp Macrocentrus cingulum

Antennal transcriptome and odorant binding protein expression profiles of an invasive mealybug and its parasitoid

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