Analysis of Oocyte-Like Cells Differentiated from Porcine Fetal Skin-Derived Stem Cells

Dyce, Paul W.; Shen, Wei; Huynh, Evanna; Shao, Hua; Villagómez, D.A.F.; Kidder, Gerald M.; King, W. A.; Li, Julang

doi:10.1089/scd.2010.0395

Cited by 48 publications

(52 citation statements)

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“…Our study, in combination with data obtained in mouse, clearly demonstrates that DAZL plays a central role in germ cell development and the progression from mitosis to meiosis. Substantial studies have reported the generation of germ cell-like cells from somatic tissue-derived stem cells such as skin [3][4][5]16,[36][37][38], bone marrow [39][40][41][42], and pancreas [43]. It has been demonstrated that epigenetic imprints are established during gametogenesis [44][45][46], and that the DNA methylation status of imprinted genes is erased and reset during PGC development [47,48].…”

Section: Discussionmentioning

confidence: 99%

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Deleted in Azoospermia-Like Enhances In Vitro Derived Porcine Germ Cell Formation and Meiosis

Park

Shen

Linher‐Melville

et al. 2013

Stem Cells and Development

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Section: Discussionmentioning

confidence: 99%

“…The PGC-like cells also undergo imprint erasure, and are capable of further differentiating into oocyte-like cells (OLCs) [3][4][5]. Using this model, we previously revealed that the heparin-binding growth factor midkine is a mitogen of PLCs [6], and its effect is partially elicited due to suppression of the Dazl gene.…”

Section: Introductionmentioning

confidence: 99%

Deleted in Azoospermia-Like Enhances In Vitro Derived Porcine Germ Cell Formation and Meiosis

Park

Shen

Linher‐Melville

et al. 2013

Stem Cells and Development

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“…Postnatal female mouse SSCs were isolated and cultured as previously described [17,18] at 37°C and 5% CO 2 air atmosphere. These stem cells have been characterized previously [18,19].…”

Section: Isolation and Culture Of Sscsmentioning

confidence: 99%

“…SSCs at the fourth day after passage 2 were isolated with the same method as previously described [17,18]. The SSCs (3 · 10 5 cells/well) were differentiated into mouse PGC-like cells (PLCs) using specific culture media consisting of highglucose DMEM (Life Technologies, Carlsbad, CA) supplemented with 5% heat-inactivated fetal bovine serum (FBS; Life Technologies; Lot No.…”

Section: In Vitro Differentiation Into Pgc-like Cells From Sscsmentioning

confidence: 99%

Ovarian-Cell-Like Cells from Skin Stem Cells Restored Estradiol Production and Estrus Cycling in Ovariectomized Mice

Park

Pan²,

Toms³

et al. 2014

Stem Cells and Development

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Reduction of estradiol production and high serum concentrations of follicular stimulating hormone (FSH) are endocrine disorders associated with premature ovarian failure. Here, we report that transplantation of ovarianlike cells differentiated from stem cells restored endogenous serum estradiol levels. Stem cells were isolated from postnatal mouse skin and differentiated into ovarian-cell-like cells that are consistent with female germ, and ovarian follicle somatic cells. The ovarian-cell-like cells were transplanted into ovariectomized mice (Cell Trans), whereas control mice were subjected to bilateral ovariectomies without cell transplantation (OVX). Using vaginal cytology analysis, it was revealed that in 13 out of 19 Cell Trans mice, estrus cycles were restored around 8 weeks after cell transplantation and were maintained until 16 weeks post-transplantation, whereas in the OVX group, all mice were arrested at metestrus/diestrus of the estrus cycle. The uterine weight in the Cell Trans group was similar to sham operation mice (Sham OP), while severe uterine atrophy and a decreased uterine weight were observed in the OVX group. Histologically, ectopic follicle-like structures and blood vessels were found within and around the transplants. At 12-14 weeks after cell transplantation, mean serum estradiol level in Cell Trans mice (178.0 -35 pg/mL) was comparable to that of the Sham OP group (188.9 -29 pg/mL), whereas it was lower in the OVX group (59.0 -4 pg/mL). Serum FSH concentration increased in the OVX group (1.62 -0.32 ng/mL) compared with the Sham OP group (0.39 -0.34 ng/mL). Cell Trans mice had a similar FSH level (0.94 -0.23 ng/mL; P < 0.05) to Sham OP mice. Our results suggest that ovarian somatic cells differentiated from stem cells are functional in vivo. In addition to providing insights into the function of ovarian somatic cells derived from stem cells, our study may offer potential therapeutic means for patients with hypo-estradiol levels like those encountered in premature ovarian failure.

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“…Estudos posteriores do mesmo grupo indicaram que esta subpopulação celular além da morfologia distinta, eram positivas para a atividade da fosfatase alcalina e expressão de mais marcadores de células germinativas como o Oct4, Fragilis, Stella, Dazl e Vasa, assim como demostraram que mesmo células derivadas de machos, formaram as células PGCs-like e estruturas semelhantes a oócitos (Dyce et al, 2011).…”

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Stem cells and animal breeding: Potential Applications

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Analysis of Oocyte-Like Cells Differentiated from Porcine Fetal Skin-Derived Stem Cells

Cited by 48 publications

References 56 publications

Deleted in Azoospermia-Like Enhances In Vitro Derived Porcine Germ Cell Formation and Meiosis

Deleted in Azoospermia-Like Enhances In Vitro Derived Porcine Germ Cell Formation and Meiosis

Ovarian-Cell-Like Cells from Skin Stem Cells Restored Estradiol Production and Estrus Cycling in Ovariectomized Mice

Stem cells and animal breeding: Potential Applications

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